Experiment: D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_p42g17 and HP15_p42g18 are separated by 50 nucleotides HP15_p42g18 and HP15_p42g19 overlap by 1 nucleotides HP15_p42g19 and HP15_p42g20 overlap by 1 nucleotides
HP15_p42g17: HP15_p42g17 - traN-like protein, at 11,174 to 12,502
_p42g17
HP15_p42g18: HP15_p42g18 - traM-like protein, at 12,553 to 12,990
_p42g18
HP15_p42g19: HP15_p42g19 - transfer origin protein, TraL, at 12,990 to 13,715
_p42g19
HP15_p42g20: HP15_p42g20 - TraK-like protein of DNA transfer, at 13,715 to 14,146
_p42g20
Position (kb) 12
13 Strain fitness (log2 ratio) -1
0
1
2
3 at 11.571 kb on + strand, within HP15_p42g17 at 11.689 kb on - strand, within HP15_p42g17 at 11.722 kb on + strand, within HP15_p42g17 at 11.966 kb on + strand, within HP15_p42g17 at 11.982 kb on + strand, within HP15_p42g17 at 11.990 kb on - strand, within HP15_p42g17 at 12.022 kb on + strand, within HP15_p42g17 at 12.050 kb on - strand, within HP15_p42g17 at 12.146 kb on - strand, within HP15_p42g17 at 12.190 kb on + strand, within HP15_p42g17 at 12.190 kb on + strand, within HP15_p42g17 at 12.205 kb on - strand, within HP15_p42g17 at 12.212 kb on + strand, within HP15_p42g17 at 12.222 kb on + strand, within HP15_p42g17 at 12.265 kb on - strand, within HP15_p42g17 at 12.268 kb on + strand, within HP15_p42g17 at 12.268 kb on + strand, within HP15_p42g17 at 12.268 kb on + strand, within HP15_p42g17 at 12.268 kb on + strand, within HP15_p42g17 at 12.268 kb on + strand, within HP15_p42g17 at 12.276 kb on - strand, within HP15_p42g17 at 12.276 kb on - strand, within HP15_p42g17 at 12.305 kb on - strand, within HP15_p42g17 at 12.318 kb on + strand, within HP15_p42g17 at 12.365 kb on - strand, within HP15_p42g17 at 12.374 kb on + strand at 12.392 kb on - strand at 12.450 kb on + strand at 12.475 kb on - strand at 12.479 kb on + strand at 12.487 kb on - strand at 12.502 kb on - strand at 12.502 kb on - strand at 12.627 kb on + strand, within HP15_p42g18 at 12.627 kb on + strand, within HP15_p42g18 at 12.646 kb on - strand, within HP15_p42g18 at 12.685 kb on - strand, within HP15_p42g18 at 12.809 kb on - strand, within HP15_p42g18 at 12.879 kb on + strand, within HP15_p42g18 at 12.933 kb on + strand, within HP15_p42g18 at 12.933 kb on + strand, within HP15_p42g18 at 12.935 kb on + strand, within HP15_p42g18 at 12.935 kb on + strand, within HP15_p42g18 at 12.935 kb on + strand, within HP15_p42g18 at 12.935 kb on + strand, within HP15_p42g18 at 12.941 kb on - strand, within HP15_p42g18 at 12.943 kb on + strand, within HP15_p42g18 at 12.998 kb on - strand at 13.008 kb on + strand at 13.140 kb on + strand, within HP15_p42g19 at 13.244 kb on + strand, within HP15_p42g19 at 13.255 kb on + strand, within HP15_p42g19 at 13.255 kb on + strand, within HP15_p42g19 at 13.257 kb on - strand, within HP15_p42g19 at 13.295 kb on + strand, within HP15_p42g19 at 13.349 kb on + strand, within HP15_p42g19 at 13.441 kb on + strand, within HP15_p42g19 at 13.540 kb on + strand, within HP15_p42g19 at 13.565 kb on + strand, within HP15_p42g19 at 13.574 kb on + strand, within HP15_p42g19 at 13.623 kb on - strand, within HP15_p42g19 at 13.638 kb on + strand, within HP15_p42g19 at 13.764 kb on + strand, within HP15_p42g20
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine remove 11,571 + HP15_p42g17 0.30 +2.2 11,689 - HP15_p42g17 0.39 +1.3 11,722 + HP15_p42g17 0.41 +2.1 11,966 + HP15_p42g17 0.60 +2.2 11,982 + HP15_p42g17 0.61 +1.2 11,990 - HP15_p42g17 0.61 +1.1 12,022 + HP15_p42g17 0.64 +3.2 12,050 - HP15_p42g17 0.66 +1.6 12,146 - HP15_p42g17 0.73 +1.9 12,190 + HP15_p42g17 0.76 +2.8 12,190 + HP15_p42g17 0.76 +1.6 12,205 - HP15_p42g17 0.78 +1.6 12,212 + HP15_p42g17 0.78 +1.2 12,222 + HP15_p42g17 0.79 +2.7 12,265 - HP15_p42g17 0.82 +1.4 12,268 + HP15_p42g17 0.82 +1.6 12,268 + HP15_p42g17 0.82 +1.7 12,268 + HP15_p42g17 0.82 +3.4 12,268 + HP15_p42g17 0.82 +3.5 12,268 + HP15_p42g17 0.82 +1.0 12,276 - HP15_p42g17 0.83 +1.2 12,276 - HP15_p42g17 0.83 +2.4 12,305 - HP15_p42g17 0.85 +3.6 12,318 + HP15_p42g17 0.86 +1.1 12,365 - HP15_p42g17 0.90 +3.7 12,374 + +2.4 12,392 - +0.6 12,450 + +1.4 12,475 - +1.4 12,479 + +1.9 12,487 - +1.8 12,502 - +0.0 12,502 - +0.7 12,627 + HP15_p42g18 0.17 -0.2 12,627 + HP15_p42g18 0.17 -0.5 12,646 - HP15_p42g18 0.21 +0.7 12,685 - HP15_p42g18 0.30 +0.4 12,809 - HP15_p42g18 0.58 -0.3 12,879 + HP15_p42g18 0.74 +1.0 12,933 + HP15_p42g18 0.87 +0.5 12,933 + HP15_p42g18 0.87 -0.5 12,935 + HP15_p42g18 0.87 +0.2 12,935 + HP15_p42g18 0.87 -1.0 12,935 + HP15_p42g18 0.87 +0.9 12,935 + HP15_p42g18 0.87 -0.0 12,941 - HP15_p42g18 0.89 -0.7 12,943 + HP15_p42g18 0.89 -0.6 12,998 - -0.9 13,008 + +0.5 13,140 + HP15_p42g19 0.21 +0.2 13,244 + HP15_p42g19 0.35 +0.3 13,255 + HP15_p42g19 0.37 +0.8 13,255 + HP15_p42g19 0.37 -0.1 13,257 - HP15_p42g19 0.37 -0.8 13,295 + HP15_p42g19 0.42 +0.5 13,349 + HP15_p42g19 0.49 -0.5 13,441 + HP15_p42g19 0.62 -0.5 13,540 + HP15_p42g19 0.76 -0.3 13,565 + HP15_p42g19 0.79 +0.7 13,574 + HP15_p42g19 0.80 -0.7 13,623 - HP15_p42g19 0.87 -0.7 13,638 + HP15_p42g19 0.89 -0.0 13,764 + HP15_p42g20 0.11 -0.3
Or see this region's nucleotide sequence