Experiment: D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3190 and HP15_3191 are separated by 125 nucleotides HP15_3191 and HP15_3192 overlap by 1 nucleotides HP15_3192 and HP15_3193 overlap by 4 nucleotides
HP15_3190: HP15_3190 - polyhydroxyalkanoic acid system protein, at 3,345,937 to 3,346,215
_3190
HP15_3191: HP15_3191 - ubiquinone/menaquinone biosynthesis methyltransferase UbiE, at 3,346,341 to 3,347,132
_3191
HP15_3192: HP15_3192 - protein containing sterol-binding domain, at 3,347,132 to 3,347,773
_3192
HP15_3193: HP15_3193 - ubiquinone biosynthesis protein UbiB, at 3,347,770 to 3,349,413
_3193
Position (kb) 3347
3348 Strain fitness (log2 ratio) -1
0
1
2 at 3346.143 kb on - strand, within HP15_3190 at 3346.176 kb on + strand, within HP15_3190 at 3346.269 kb on - strand at 3346.279 kb on + strand at 3347.152 kb on + strand at 3347.171 kb on + strand at 3347.173 kb on + strand at 3347.181 kb on - strand at 3347.279 kb on + strand, within HP15_3192 at 3347.284 kb on + strand, within HP15_3192 at 3347.284 kb on + strand, within HP15_3192 at 3347.767 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine remove 3,346,143 - HP15_3190 0.74 +1.8 3,346,176 + HP15_3190 0.86 -0.5 3,346,269 - -1.1 3,346,279 + -0.1 3,347,152 + +0.2 3,347,171 + +0.5 3,347,173 + -0.8 3,347,181 - +0.5 3,347,279 + HP15_3192 0.23 -0.5 3,347,284 + HP15_3192 0.24 -0.8 3,347,284 + HP15_3192 0.24 -0.3 3,347,767 + -0.6
Or see this region's nucleotide sequence