Experiment: D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3084 and HP15_3085 are separated by 104 nucleotides HP15_3085 and HP15_3086 are separated by 122 nucleotides HP15_3086 and HP15_3087 overlap by 7 nucleotides HP15_3087 and HP15_3088 overlap by 4 nucleotides HP15_3088 and HP15_3089 are separated by 72 nucleotides
HP15_3084: HP15_3084 - DNA repair protein RecN, at 3,239,819 to 3,241,498
_3084
HP15_3085: HP15_3085 - ferric-uptake regulator, at 3,241,603 to 3,242,013
_3085
HP15_3086: HP15_3086 - outer membrane lipoprotein OmlA, at 3,242,136 to 3,242,462
_3086
HP15_3087: HP15_3087 - protein belonging to uncharacterized protein family UPF0125, at 3,242,456 to 3,242,746
_3087
HP15_3088: HP15_3088 - oligoketide cyclase/lipid transport protein, at 3,242,743 to 3,243,189
_3088
HP15_3089: HP15_3089 - sodium-dependent transporter family protein, at 3,243,262 to 3,244,821
_3089
Position (kb)
3242
3243 Strain fitness (log2 ratio)
-2
-1
0
1 at 3241.174 kb on - strand, within HP15_3084 at 3241.180 kb on - strand, within HP15_3084 at 3241.184 kb on - strand, within HP15_3084 at 3241.189 kb on - strand, within HP15_3084 at 3241.324 kb on - strand, within HP15_3084 at 3241.338 kb on + strand at 3241.429 kb on + strand at 3241.459 kb on - strand at 3241.487 kb on + strand at 3241.495 kb on - strand at 3241.495 kb on - strand at 3241.514 kb on + strand at 3241.547 kb on - strand at 3241.598 kb on - strand at 3242.409 kb on + strand, within HP15_3086 at 3242.456 kb on + strand at 3242.538 kb on - strand, within HP15_3087 at 3242.562 kb on - strand, within HP15_3087 at 3242.659 kb on + strand, within HP15_3087 at 3242.678 kb on - strand, within HP15_3087 at 3242.767 kb on - strand at 3242.914 kb on - strand, within HP15_3088 at 3242.978 kb on + strand, within HP15_3088 at 3243.041 kb on + strand, within HP15_3088 at 3243.159 kb on - strand at 3243.195 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine remove 3,241,174 - HP15_3084 0.81 +0.3 3,241,180 - HP15_3084 0.81 +1.2 3,241,184 - HP15_3084 0.81 +0.9 3,241,189 - HP15_3084 0.82 -0.3 3,241,324 - HP15_3084 0.90 -0.1 3,241,338 + +0.1 3,241,429 + +0.7 3,241,459 - -0.1 3,241,487 + +0.2 3,241,495 - +0.7 3,241,495 - +0.3 3,241,514 + -0.4 3,241,547 - -2.7 3,241,598 - -0.5 3,242,409 + HP15_3086 0.83 -0.5 3,242,456 + +0.8 3,242,538 - HP15_3087 0.28 -0.7 3,242,562 - HP15_3087 0.36 +0.1 3,242,659 + HP15_3087 0.70 -0.7 3,242,678 - HP15_3087 0.76 +0.9 3,242,767 - +0.4 3,242,914 - HP15_3088 0.38 +0.3 3,242,978 + HP15_3088 0.53 -0.5 3,243,041 + HP15_3088 0.67 +0.3 3,243,159 - +0.6 3,243,195 + +0.5
Or see this region's nucleotide sequence