Experiment: D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3001 and HP15_3002 are separated by 22 nucleotides HP15_3002 and HP15_3003 are separated by 143 nucleotides HP15_3003 and HP15_3004 are separated by 144 nucleotides
HP15_3001: HP15_3001 - conserved hypothetical protein, at 3,144,758 to 3,145,897
_3001
HP15_3002: HP15_3002 - TonB-dependent receptor, at 3,145,920 to 3,147,929
_3002
HP15_3003: HP15_3003 - protein-export protein SecB, at 3,148,073 to 3,148,576
_3003
HP15_3004: HP15_3004 - rhodanese domain protein, at 3,148,721 to 3,149,137
_3004
Position (kb)
3145
3146
3147
3148 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3144.995 kb on + strand, within HP15_3001 at 3144.995 kb on - strand, within HP15_3001 at 3145.027 kb on - strand, within HP15_3001 at 3145.078 kb on - strand, within HP15_3001 at 3145.137 kb on + strand, within HP15_3001 at 3145.205 kb on + strand, within HP15_3001 at 3145.205 kb on + strand, within HP15_3001 at 3145.237 kb on + strand, within HP15_3001 at 3145.260 kb on + strand, within HP15_3001 at 3145.277 kb on + strand, within HP15_3001 at 3145.301 kb on + strand, within HP15_3001 at 3145.301 kb on + strand, within HP15_3001 at 3145.301 kb on + strand, within HP15_3001 at 3145.307 kb on + strand, within HP15_3001 at 3145.374 kb on - strand, within HP15_3001 at 3145.390 kb on + strand, within HP15_3001 at 3145.417 kb on - strand, within HP15_3001 at 3145.634 kb on - strand, within HP15_3001 at 3145.646 kb on - strand, within HP15_3001 at 3145.746 kb on + strand, within HP15_3001 at 3145.774 kb on + strand, within HP15_3001 at 3145.794 kb on + strand at 3145.802 kb on - strand at 3145.948 kb on + strand at 3146.041 kb on + strand at 3146.053 kb on + strand at 3146.059 kb on - strand at 3146.109 kb on - strand at 3146.110 kb on - strand at 3146.158 kb on + strand, within HP15_3002 at 3146.158 kb on + strand, within HP15_3002 at 3146.158 kb on + strand, within HP15_3002 at 3146.158 kb on + strand, within HP15_3002 at 3146.158 kb on + strand, within HP15_3002 at 3146.166 kb on - strand, within HP15_3002 at 3146.166 kb on - strand, within HP15_3002 at 3146.166 kb on - strand, within HP15_3002 at 3146.166 kb on - strand, within HP15_3002 at 3146.166 kb on - strand, within HP15_3002 at 3146.262 kb on + strand, within HP15_3002 at 3146.275 kb on - strand, within HP15_3002 at 3146.275 kb on - strand, within HP15_3002 at 3146.343 kb on - strand, within HP15_3002 at 3146.416 kb on + strand, within HP15_3002 at 3146.424 kb on - strand, within HP15_3002 at 3146.459 kb on - strand, within HP15_3002 at 3146.481 kb on + strand, within HP15_3002 at 3146.546 kb on - strand, within HP15_3002 at 3146.575 kb on + strand, within HP15_3002 at 3146.687 kb on - strand, within HP15_3002 at 3146.695 kb on - strand, within HP15_3002 at 3146.698 kb on + strand, within HP15_3002 at 3146.769 kb on + strand, within HP15_3002 at 3146.801 kb on + strand, within HP15_3002 at 3146.899 kb on - strand, within HP15_3002 at 3147.074 kb on + strand, within HP15_3002 at 3147.219 kb on + strand, within HP15_3002 at 3147.264 kb on - strand, within HP15_3002 at 3147.493 kb on + strand, within HP15_3002 at 3147.517 kb on + strand, within HP15_3002 at 3147.609 kb on + strand, within HP15_3002 at 3148.196 kb on + strand, within HP15_3003 at 3148.289 kb on + strand, within HP15_3003 at 3148.331 kb on + strand, within HP15_3003 at 3148.388 kb on - strand, within HP15_3003 at 3148.388 kb on - strand, within HP15_3003 at 3148.395 kb on + strand, within HP15_3003 at 3148.470 kb on + strand, within HP15_3003 at 3148.487 kb on + strand, within HP15_3003 at 3148.609 kb on + strand at 3148.628 kb on + strand at 3148.634 kb on - strand at 3148.641 kb on - strand at 3148.877 kb on - strand, within HP15_3004 at 3148.910 kb on + strand, within HP15_3004 at 3148.910 kb on + strand, within HP15_3004
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine remove 3,144,995 + HP15_3001 0.21 -1.0 3,144,995 - HP15_3001 0.21 -0.0 3,145,027 - HP15_3001 0.24 +0.4 3,145,078 - HP15_3001 0.28 +0.4 3,145,137 + HP15_3001 0.33 +0.5 3,145,205 + HP15_3001 0.39 -1.2 3,145,205 + HP15_3001 0.39 -0.8 3,145,237 + HP15_3001 0.42 -0.6 3,145,260 + HP15_3001 0.44 +0.2 3,145,277 + HP15_3001 0.46 -0.9 3,145,301 + HP15_3001 0.48 +0.6 3,145,301 + HP15_3001 0.48 +2.5 3,145,301 + HP15_3001 0.48 +0.5 3,145,307 + HP15_3001 0.48 -1.0 3,145,374 - HP15_3001 0.54 -0.7 3,145,390 + HP15_3001 0.55 +0.1 3,145,417 - HP15_3001 0.58 -0.3 3,145,634 - HP15_3001 0.77 +0.8 3,145,646 - HP15_3001 0.78 +0.6 3,145,746 + HP15_3001 0.87 -0.7 3,145,774 + HP15_3001 0.89 -0.5 3,145,794 + -1.6 3,145,802 - -0.3 3,145,948 + -1.3 3,146,041 + -2.0 3,146,053 + -0.2 3,146,059 - -0.4 3,146,109 - -0.3 3,146,110 - -1.3 3,146,158 + HP15_3002 0.12 +0.2 3,146,158 + HP15_3002 0.12 -0.2 3,146,158 + HP15_3002 0.12 -0.2 3,146,158 + HP15_3002 0.12 +0.8 3,146,158 + HP15_3002 0.12 -0.2 3,146,166 - HP15_3002 0.12 -0.4 3,146,166 - HP15_3002 0.12 +0.0 3,146,166 - HP15_3002 0.12 -0.6 3,146,166 - HP15_3002 0.12 -0.2 3,146,166 - HP15_3002 0.12 -1.9 3,146,262 + HP15_3002 0.17 -0.3 3,146,275 - HP15_3002 0.18 +0.2 3,146,275 - HP15_3002 0.18 +0.2 3,146,343 - HP15_3002 0.21 +0.1 3,146,416 + HP15_3002 0.25 -0.1 3,146,424 - HP15_3002 0.25 -0.9 3,146,459 - HP15_3002 0.27 -0.5 3,146,481 + HP15_3002 0.28 -0.5 3,146,546 - HP15_3002 0.31 -1.8 3,146,575 + HP15_3002 0.33 -0.7 3,146,687 - HP15_3002 0.38 +0.1 3,146,695 - HP15_3002 0.39 -1.3 3,146,698 + HP15_3002 0.39 -0.7 3,146,769 + HP15_3002 0.42 +0.3 3,146,801 + HP15_3002 0.44 -0.8 3,146,899 - HP15_3002 0.49 -0.4 3,147,074 + HP15_3002 0.57 -1.3 3,147,219 + HP15_3002 0.65 -0.0 3,147,264 - HP15_3002 0.67 -0.8 3,147,493 + HP15_3002 0.78 +0.2 3,147,517 + HP15_3002 0.79 +0.8 3,147,609 + HP15_3002 0.84 -1.0 3,148,196 + HP15_3003 0.24 +0.5 3,148,289 + HP15_3003 0.43 +0.4 3,148,331 + HP15_3003 0.51 +1.4 3,148,388 - HP15_3003 0.62 -0.8 3,148,388 - HP15_3003 0.62 +0.7 3,148,395 + HP15_3003 0.64 +0.1 3,148,470 + HP15_3003 0.79 +0.0 3,148,487 + HP15_3003 0.82 +0.4 3,148,609 + -0.2 3,148,628 + -0.2 3,148,634 - +0.5 3,148,641 - +0.0 3,148,877 - HP15_3004 0.37 -0.2 3,148,910 + HP15_3004 0.45 -2.5 3,148,910 + HP15_3004 0.45 -0.6
Or see this region's nucleotide sequence