Experiment: D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_296 and HP15_297 are separated by 95 nucleotides HP15_297 and HP15_298 are separated by 3 nucleotides HP15_298 and HP15_299 are separated by 81 nucleotides
HP15_296: HP15_296 - conserved hypothetical protein, at 288,252 to 288,674
_296
HP15_297: HP15_297 - ATPase components of ABC transporters with duplicated ATPase domains, at 288,770 to 289,423
_297
HP15_298: HP15_298 - orotate phosphoribosyltransferase, at 289,427 to 290,065
_298
HP15_299: HP15_299 - exodeoxyribonuclease III Xth, at 290,147 to 290,953
_299
Position (kb)
289
290
291 Strain fitness (log2 ratio)
-1
0
1 at 288.519 kb on + strand, within HP15_296 at 288.615 kb on + strand, within HP15_296 at 288.615 kb on + strand, within HP15_296 at 288.648 kb on + strand at 288.653 kb on + strand at 288.780 kb on + strand at 288.780 kb on + strand at 288.788 kb on - strand at 288.788 kb on - strand at 288.944 kb on - strand, within HP15_297 at 289.003 kb on - strand, within HP15_297 at 289.059 kb on - strand, within HP15_297 at 289.316 kb on + strand, within HP15_297 at 289.324 kb on - strand, within HP15_297 at 289.376 kb on + strand at 289.396 kb on - strand at 289.530 kb on - strand, within HP15_298 at 289.710 kb on + strand, within HP15_298 at 289.932 kb on - strand, within HP15_298 at 289.992 kb on + strand, within HP15_298 at 290.794 kb on + strand, within HP15_299 at 290.980 kb on + strand at 290.988 kb on + strand at 291.018 kb on - strand at 291.033 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine remove 288,519 + HP15_296 0.63 +0.0 288,615 + HP15_296 0.86 -0.6 288,615 + HP15_296 0.86 -1.1 288,648 + +0.7 288,653 + -0.6 288,780 + -0.1 288,780 + -0.6 288,788 - -0.7 288,788 - -0.3 288,944 - HP15_297 0.27 -0.2 289,003 - HP15_297 0.36 +0.3 289,059 - HP15_297 0.44 -0.3 289,316 + HP15_297 0.83 +1.7 289,324 - HP15_297 0.85 +0.3 289,376 + +1.3 289,396 - +0.1 289,530 - HP15_298 0.16 -1.7 289,710 + HP15_298 0.44 -0.7 289,932 - HP15_298 0.79 -1.3 289,992 + HP15_298 0.88 -0.8 290,794 + HP15_299 0.80 -0.3 290,980 + -1.3 290,988 + +0.7 291,018 - +0.5 291,033 - +0.3
Or see this region's nucleotide sequence