Experiment: D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2196 and HP15_2197 are separated by 243 nucleotides HP15_2197 and HP15_2198 are separated by 33 nucleotides HP15_2198 and HP15_2199 are separated by 68 nucleotides
HP15_2196: HP15_2196 - glutamate/glutamine/aspartate/asparagine ABC transporter, periplasmic substrate-binding protein, at 2,313,336 to 2,314,379
_2196
HP15_2197: HP15_2197 - conserved hypothetical protein, secreted, at 2,314,623 to 2,315,510
_2197
HP15_2198: HP15_2198 - thioesterase superfamily protein, at 2,315,544 to 2,315,936
_2198
HP15_2199: HP15_2199 - glucose/sorbosone dehydrogenase, at 2,316,005 to 2,317,540
_2199
Position (kb)
2314
2315
2316 Strain fitness (log2 ratio)
-1
0
1
2 at 2313.702 kb on + strand, within HP15_2196 at 2313.779 kb on - strand, within HP15_2196 at 2313.801 kb on + strand, within HP15_2196 at 2313.803 kb on + strand, within HP15_2196 at 2313.803 kb on + strand, within HP15_2196 at 2313.823 kb on - strand, within HP15_2196 at 2313.932 kb on + strand, within HP15_2196 at 2313.980 kb on - strand, within HP15_2196 at 2314.029 kb on + strand, within HP15_2196 at 2314.105 kb on - strand, within HP15_2196 at 2314.105 kb on - strand, within HP15_2196 at 2314.316 kb on - strand at 2314.524 kb on - strand at 2314.797 kb on - strand, within HP15_2197 at 2314.827 kb on + strand, within HP15_2197 at 2314.830 kb on - strand, within HP15_2197 at 2314.830 kb on - strand, within HP15_2197 at 2314.981 kb on - strand, within HP15_2197 at 2315.366 kb on - strand, within HP15_2197 at 2315.508 kb on + strand at 2315.748 kb on + strand, within HP15_2198 at 2315.796 kb on + strand, within HP15_2198 at 2315.839 kb on + strand, within HP15_2198 at 2315.839 kb on + strand, within HP15_2198 at 2315.847 kb on - strand, within HP15_2198 at 2315.847 kb on - strand, within HP15_2198 at 2315.966 kb on + strand at 2315.989 kb on - strand at 2316.280 kb on + strand, within HP15_2199 at 2316.328 kb on + strand, within HP15_2199
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine remove 2,313,702 + HP15_2196 0.35 -0.7 2,313,779 - HP15_2196 0.42 -0.0 2,313,801 + HP15_2196 0.45 +0.3 2,313,803 + HP15_2196 0.45 +0.4 2,313,803 + HP15_2196 0.45 +0.6 2,313,823 - HP15_2196 0.47 +0.6 2,313,932 + HP15_2196 0.57 +0.2 2,313,980 - HP15_2196 0.62 -0.1 2,314,029 + HP15_2196 0.66 +0.1 2,314,105 - HP15_2196 0.74 -0.1 2,314,105 - HP15_2196 0.74 -0.8 2,314,316 - +0.7 2,314,524 - -0.6 2,314,797 - HP15_2197 0.20 -0.1 2,314,827 + HP15_2197 0.23 -0.9 2,314,830 - HP15_2197 0.23 -1.0 2,314,830 - HP15_2197 0.23 -1.5 2,314,981 - HP15_2197 0.40 -0.6 2,315,366 - HP15_2197 0.84 -0.5 2,315,508 + +2.2 2,315,748 + HP15_2198 0.52 -1.1 2,315,796 + HP15_2198 0.64 -0.4 2,315,839 + HP15_2198 0.75 -0.5 2,315,839 + HP15_2198 0.75 -0.7 2,315,847 - HP15_2198 0.77 +0.6 2,315,847 - HP15_2198 0.77 +1.1 2,315,966 + +0.7 2,315,989 - +0.3 2,316,280 + HP15_2199 0.18 +1.3 2,316,328 + HP15_2199 0.21 -0.1
Or see this region's nucleotide sequence