Experiment: D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2184 and HP15_2185 are separated by 76 nucleotides HP15_2185 and HP15_2186 are separated by 204 nucleotides HP15_2186 and HP15_tRNA38 are separated by 222 nucleotides HP15_tRNA38 and HP15_tRNA39 are separated by 79 nucleotides HP15_tRNA39 and HP15_tRNA40 are separated by 75 nucleotides HP15_tRNA40 and HP15_tRNA41 are separated by 33 nucleotides HP15_tRNA41 and HP15_2187 are separated by 189 nucleotides
HP15_2184: HP15_2184 - ATP-dependent Clp protease ATP-binding subunit ClpX, at 2,297,994 to 2,299,277
_2184
HP15_2185: HP15_2185 - ATP-dependent Clp protease, proteolytic subunit ClpP, at 2,299,354 to 2,299,959
_2185
HP15_2186: HP15_2186 - trigger factor, at 2,300,164 to 2,301,468
_2186
HP15_tRNA38: HP15_tRNA38 - tRNA-Leu, at 2,301,691 to 2,301,775
_tRNA38
HP15_tRNA39: HP15_tRNA39 - tRNA-His, at 2,301,855 to 2,301,930
_tRNA39
HP15_tRNA40: HP15_tRNA40 - tRNA-Arg, at 2,302,006 to 2,302,082
_tRNA40
HP15_tRNA41: HP15_tRNA41 - tRNA-Pro, at 2,302,116 to 2,302,192
_tRNA41
HP15_2187: HP15_2187 - methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, at 2,302,382 to 2,303,284
_2187
Position (kb)
2300
2301
2302 Strain fitness (log2 ratio)
-2
-1
0
1 at 2300.018 kb on - strand at 2300.240 kb on - strand at 2300.311 kb on - strand, within HP15_2186 at 2300.412 kb on + strand, within HP15_2186 at 2300.422 kb on - strand, within HP15_2186 at 2300.587 kb on - strand, within HP15_2186 at 2300.696 kb on + strand, within HP15_2186 at 2300.803 kb on + strand, within HP15_2186 at 2300.811 kb on - strand, within HP15_2186 at 2300.839 kb on + strand, within HP15_2186 at 2301.236 kb on + strand, within HP15_2186 at 2301.237 kb on + strand, within HP15_2186 at 2301.239 kb on + strand, within HP15_2186 at 2301.293 kb on + strand, within HP15_2186 at 2301.605 kb on - strand at 2301.630 kb on + strand at 2301.695 kb on - strand at 2302.322 kb on + strand at 2302.330 kb on - strand at 2302.337 kb on + strand at 2302.345 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine remove 2,300,018 - +0.2 2,300,240 - +0.3 2,300,311 - HP15_2186 0.11 -0.8 2,300,412 + HP15_2186 0.19 -0.9 2,300,422 - HP15_2186 0.20 -0.4 2,300,587 - HP15_2186 0.32 -0.6 2,300,696 + HP15_2186 0.41 -0.3 2,300,803 + HP15_2186 0.49 -0.9 2,300,811 - HP15_2186 0.50 -2.2 2,300,839 + HP15_2186 0.52 +0.5 2,301,236 + HP15_2186 0.82 -1.0 2,301,237 + HP15_2186 0.82 -0.1 2,301,239 + HP15_2186 0.82 +0.6 2,301,293 + HP15_2186 0.87 -0.6 2,301,605 - -0.0 2,301,630 + -0.7 2,301,695 - +0.1 2,302,322 + -0.3 2,302,330 - -0.1 2,302,337 + -0.3 2,302,345 - +1.4
Or see this region's nucleotide sequence