Experiment: D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2029 and HP15_2030 are separated by 16 nucleotides HP15_2030 and HP15_2031 are separated by 203 nucleotides HP15_2031 and HP15_2032 are separated by 0 nucleotides
HP15_2029: HP15_2029 - permease perM, at 2,129,258 to 2,130,337
_2029
HP15_2030: HP15_2030 - protein containing SirA-like domain, at 2,130,354 to 2,130,581
_2030
HP15_2031: HP15_2031 - putative Zn-dependent protease, contains TPR repeats, at 2,130,785 to 2,132,217
_2031
HP15_2032: HP15_2032 - quinolinate synthetase complex, A subunit, at 2,132,218 to 2,133,279
_2032
Position (kb)
2130
2131
2132
2133 Strain fitness (log2 ratio)
-2
-1
0
1 at 2129.791 kb on + strand, within HP15_2029 at 2129.794 kb on - strand, within HP15_2029 at 2129.871 kb on + strand, within HP15_2029 at 2129.925 kb on - strand, within HP15_2029 at 2130.192 kb on - strand, within HP15_2029 at 2130.207 kb on + strand, within HP15_2029 at 2130.267 kb on + strand at 2130.663 kb on + strand at 2130.747 kb on - strand at 2130.803 kb on - strand at 2131.079 kb on - strand, within HP15_2031 at 2131.172 kb on - strand, within HP15_2031 at 2131.172 kb on - strand, within HP15_2031 at 2131.337 kb on - strand, within HP15_2031 at 2131.643 kb on + strand, within HP15_2031 at 2131.723 kb on - strand, within HP15_2031 at 2132.116 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine remove 2,129,791 + HP15_2029 0.49 -0.7 2,129,794 - HP15_2029 0.50 +1.3 2,129,871 + HP15_2029 0.57 -0.7 2,129,925 - HP15_2029 0.62 -0.1 2,130,192 - HP15_2029 0.86 -0.9 2,130,207 + HP15_2029 0.88 -0.0 2,130,267 + +0.8 2,130,663 + -0.2 2,130,747 - -0.7 2,130,803 - -0.7 2,131,079 - HP15_2031 0.21 -1.7 2,131,172 - HP15_2031 0.27 -1.1 2,131,172 - HP15_2031 0.27 -0.1 2,131,337 - HP15_2031 0.39 -1.5 2,131,643 + HP15_2031 0.60 -2.7 2,131,723 - HP15_2031 0.65 -0.7 2,132,116 - -1.2
Or see this region's nucleotide sequence