Experiment: D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1967 and HP15_1968 are separated by 79 nucleotides HP15_1968 and HP15_1969 are separated by 116 nucleotides HP15_1969 and HP15_1970 are separated by 251 nucleotides
HP15_1967: HP15_1967 - phage terminase large subunit (GpA), at 2,084,180 to 2,086,012
_1967
HP15_1968: HP15_1968 - phage DNA packaging protein, Nu1 subunit of terminase, at 2,086,092 to 2,086,610
_1968
HP15_1969: HP15_1969 - hypothetical protein, at 2,086,727 to 2,086,831
_1969
HP15_1970: HP15_1970 - hypothetical protein, at 2,087,083 to 2,088,630
_1970
Position (kb)
2086
2087 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2085.101 kb on - strand, within HP15_1967 at 2085.170 kb on + strand, within HP15_1967 at 2085.170 kb on + strand, within HP15_1967 at 2085.178 kb on - strand, within HP15_1967 at 2085.216 kb on - strand, within HP15_1967 at 2085.225 kb on + strand, within HP15_1967 at 2085.230 kb on + strand, within HP15_1967 at 2085.299 kb on - strand, within HP15_1967 at 2085.523 kb on + strand, within HP15_1967 at 2085.526 kb on - strand, within HP15_1967 at 2085.531 kb on + strand, within HP15_1967 at 2085.635 kb on - strand, within HP15_1967 at 2085.659 kb on - strand, within HP15_1967 at 2085.659 kb on - strand, within HP15_1967 at 2085.751 kb on + strand, within HP15_1967 at 2085.774 kb on - strand, within HP15_1967 at 2085.953 kb on + strand at 2085.981 kb on + strand at 2086.034 kb on + strand at 2086.034 kb on + strand at 2086.042 kb on - strand at 2086.058 kb on + strand at 2086.061 kb on - strand at 2086.116 kb on + strand at 2086.133 kb on + strand at 2086.133 kb on + strand at 2086.141 kb on - strand at 2086.291 kb on - strand, within HP15_1968 at 2086.329 kb on - strand, within HP15_1968 at 2086.387 kb on + strand, within HP15_1968 at 2086.507 kb on + strand, within HP15_1968 at 2086.644 kb on + strand at 2086.652 kb on - strand at 2086.652 kb on - strand at 2086.693 kb on + strand at 2086.788 kb on + strand, within HP15_1969 at 2086.815 kb on + strand, within HP15_1969 at 2086.833 kb on + strand at 2086.846 kb on - strand at 2086.847 kb on - strand at 2086.847 kb on - strand at 2086.926 kb on - strand at 2086.928 kb on + strand at 2087.053 kb on + strand at 2087.053 kb on + strand at 2087.075 kb on - strand at 2087.087 kb on - strand at 2087.108 kb on + strand at 2087.130 kb on - strand at 2087.140 kb on - strand at 2087.175 kb on + strand at 2087.175 kb on + strand at 2087.183 kb on - strand at 2087.183 kb on - strand at 2087.183 kb on - strand at 2087.183 kb on - strand at 2087.299 kb on + strand, within HP15_1970 at 2087.304 kb on + strand, within HP15_1970 at 2087.304 kb on + strand, within HP15_1970 at 2087.314 kb on - strand, within HP15_1970 at 2087.323 kb on + strand, within HP15_1970 at 2087.351 kb on - strand, within HP15_1970 at 2087.357 kb on - strand, within HP15_1970 at 2087.390 kb on + strand, within HP15_1970 at 2087.392 kb on + strand, within HP15_1970 at 2087.478 kb on - strand, within HP15_1970 at 2087.558 kb on - strand, within HP15_1970
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine remove 2,085,101 - HP15_1967 0.50 +0.6 2,085,170 + HP15_1967 0.54 -0.1 2,085,170 + HP15_1967 0.54 -0.1 2,085,178 - HP15_1967 0.54 -1.7 2,085,216 - HP15_1967 0.57 -0.4 2,085,225 + HP15_1967 0.57 -1.3 2,085,230 + HP15_1967 0.57 -0.0 2,085,299 - HP15_1967 0.61 -0.2 2,085,523 + HP15_1967 0.73 +0.1 2,085,526 - HP15_1967 0.73 +0.0 2,085,531 + HP15_1967 0.74 +0.5 2,085,635 - HP15_1967 0.79 +1.6 2,085,659 - HP15_1967 0.81 +1.1 2,085,659 - HP15_1967 0.81 -1.3 2,085,751 + HP15_1967 0.86 +1.5 2,085,774 - HP15_1967 0.87 -0.2 2,085,953 + +1.1 2,085,981 + +0.6 2,086,034 + -0.0 2,086,034 + +1.9 2,086,042 - -1.8 2,086,058 + -1.2 2,086,061 - +0.4 2,086,116 + +0.1 2,086,133 + +0.2 2,086,133 + -1.3 2,086,141 - +1.0 2,086,291 - HP15_1968 0.38 -0.7 2,086,329 - HP15_1968 0.46 -0.8 2,086,387 + HP15_1968 0.57 +0.3 2,086,507 + HP15_1968 0.80 -0.4 2,086,644 + +0.7 2,086,652 - +0.6 2,086,652 - -1.3 2,086,693 + -0.7 2,086,788 + HP15_1969 0.58 +0.1 2,086,815 + HP15_1969 0.84 -0.6 2,086,833 + -0.1 2,086,846 - -0.1 2,086,847 - +0.4 2,086,847 - -0.2 2,086,926 - +0.1 2,086,928 + +0.3 2,087,053 + +0.6 2,087,053 + -0.5 2,087,075 - -0.2 2,087,087 - +0.6 2,087,108 + +1.4 2,087,130 - -0.2 2,087,140 - +0.7 2,087,175 + +0.8 2,087,175 + +0.6 2,087,183 - +0.3 2,087,183 - +1.3 2,087,183 - +0.7 2,087,183 - +0.6 2,087,299 + HP15_1970 0.14 +1.1 2,087,304 + HP15_1970 0.14 +0.3 2,087,304 + HP15_1970 0.14 -3.0 2,087,314 - HP15_1970 0.15 +0.1 2,087,323 + HP15_1970 0.16 +0.4 2,087,351 - HP15_1970 0.17 +1.0 2,087,357 - HP15_1970 0.18 +1.0 2,087,390 + HP15_1970 0.20 +0.4 2,087,392 + HP15_1970 0.20 +0.8 2,087,478 - HP15_1970 0.26 +0.3 2,087,558 - HP15_1970 0.31 +1.2
Or see this region's nucleotide sequence