Experiment: D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1863 and HP15_1864 are separated by 123 nucleotides HP15_1864 and HP15_1865 are separated by 194 nucleotides HP15_1865 and HP15_1866 are separated by 47 nucleotides
HP15_1863: HP15_1863 - RNA helicase, ATP-dependent DEAH box, HrpA type, at 1,965,940 to 1,969,875
_1863
HP15_1864: HP15_1864 - acyl-CoA synthase, at 1,969,999 to 1,971,681
_1864
HP15_1865: HP15_1865 - MaoC domain protein dehydratase, at 1,971,876 to 1,972,361
_1865
HP15_1866: HP15_1866 - conserved hypothetical protein, at 1,972,409 to 1,973,308
_1866
Position (kb)
1969
1970
1971
1972 Strain fitness (log2 ratio)
-1
0
1 at 1969.110 kb on - strand, within HP15_1863 at 1969.177 kb on + strand, within HP15_1863 at 1969.317 kb on + strand, within HP15_1863 at 1969.358 kb on - strand, within HP15_1863 at 1969.464 kb on + strand, within HP15_1863 at 1969.464 kb on + strand, within HP15_1863 at 1969.472 kb on - strand, within HP15_1863 at 1969.472 kb on - strand, within HP15_1863 at 1969.599 kb on - strand at 1969.645 kb on + strand at 1969.669 kb on + strand at 1969.778 kb on + strand at 1970.062 kb on - strand at 1970.149 kb on + strand at 1970.447 kb on + strand, within HP15_1864 at 1970.501 kb on + strand, within HP15_1864 at 1970.509 kb on - strand, within HP15_1864 at 1970.570 kb on - strand, within HP15_1864 at 1970.570 kb on - strand, within HP15_1864 at 1970.866 kb on + strand, within HP15_1864 at 1970.874 kb on - strand, within HP15_1864 at 1970.925 kb on - strand, within HP15_1864 at 1970.997 kb on - strand, within HP15_1864 at 1971.352 kb on - strand, within HP15_1864 at 1971.370 kb on + strand, within HP15_1864 at 1971.378 kb on - strand, within HP15_1864 at 1971.437 kb on + strand, within HP15_1864 at 1971.460 kb on - strand, within HP15_1864 at 1971.610 kb on + strand at 1971.824 kb on - strand at 1971.852 kb on + strand at 1971.854 kb on + strand at 1971.880 kb on + strand at 1971.890 kb on + strand at 1971.955 kb on + strand, within HP15_1865 at 1971.955 kb on + strand, within HP15_1865 at 1971.963 kb on - strand, within HP15_1865 at 1971.963 kb on - strand, within HP15_1865 at 1971.963 kb on - strand, within HP15_1865 at 1971.963 kb on - strand, within HP15_1865 at 1971.980 kb on + strand, within HP15_1865 at 1972.076 kb on - strand, within HP15_1865 at 1972.140 kb on + strand, within HP15_1865 at 1972.143 kb on - strand, within HP15_1865 at 1972.520 kb on + strand, within HP15_1866 at 1972.590 kb on + strand, within HP15_1866
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine remove 1,969,110 - HP15_1863 0.81 -1.5 1,969,177 + HP15_1863 0.82 -0.6 1,969,317 + HP15_1863 0.86 -0.5 1,969,358 - HP15_1863 0.87 -0.1 1,969,464 + HP15_1863 0.90 +0.0 1,969,464 + HP15_1863 0.90 -0.3 1,969,472 - HP15_1863 0.90 -0.6 1,969,472 - HP15_1863 0.90 -0.4 1,969,599 - +0.6 1,969,645 + +0.7 1,969,669 + +0.8 1,969,778 + +0.8 1,970,062 - -1.1 1,970,149 + -0.4 1,970,447 + HP15_1864 0.27 -0.1 1,970,501 + HP15_1864 0.30 -1.4 1,970,509 - HP15_1864 0.30 -0.9 1,970,570 - HP15_1864 0.34 +0.3 1,970,570 - HP15_1864 0.34 -0.4 1,970,866 + HP15_1864 0.52 -0.7 1,970,874 - HP15_1864 0.52 +0.1 1,970,925 - HP15_1864 0.55 -1.7 1,970,997 - HP15_1864 0.59 -1.0 1,971,352 - HP15_1864 0.80 -1.3 1,971,370 + HP15_1864 0.81 +0.3 1,971,378 - HP15_1864 0.82 +0.3 1,971,437 + HP15_1864 0.85 -1.3 1,971,460 - HP15_1864 0.87 -1.5 1,971,610 + -1.1 1,971,824 - +0.7 1,971,852 + -1.2 1,971,854 + -1.0 1,971,880 + -0.3 1,971,890 + -0.0 1,971,955 + HP15_1865 0.16 +0.7 1,971,955 + HP15_1865 0.16 +0.7 1,971,963 - HP15_1865 0.18 -0.7 1,971,963 - HP15_1865 0.18 +0.3 1,971,963 - HP15_1865 0.18 +1.2 1,971,963 - HP15_1865 0.18 -0.2 1,971,980 + HP15_1865 0.21 +0.5 1,972,076 - HP15_1865 0.41 -0.2 1,972,140 + HP15_1865 0.54 +0.0 1,972,143 - HP15_1865 0.55 -1.3 1,972,520 + HP15_1866 0.12 -0.7 1,972,590 + HP15_1866 0.20 +1.5
Or see this region's nucleotide sequence