Experiment: D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1718 and HP15_1719 are separated by 5 nucleotides HP15_1719 and HP15_1720 are separated by 25 nucleotides HP15_1720 and HP15_1721 are separated by 47 nucleotides
HP15_1718: HP15_1718 - ATP-dependent DNA helicase RecQ, at 1,816,442 to 1,818,310
_1718
HP15_1719: HP15_1719 - conserved hypothetical protein, at 1,818,316 to 1,818,828
_1719
HP15_1720: HP15_1720 - hypothetical protein, at 1,818,854 to 1,819,009
_1720
HP15_1721: HP15_1721 - conserved hypothetical protein, secreted, at 1,819,057 to 1,820,013
_1721
Position (kb)
1818
1819
1820 Strain fitness (log2 ratio)
-1
0
1 at 1817.892 kb on + strand, within HP15_1718 at 1817.975 kb on - strand, within HP15_1718 at 1818.304 kb on + strand at 1818.334 kb on - strand at 1818.691 kb on - strand, within HP15_1719 at 1818.693 kb on + strand, within HP15_1719 at 1818.693 kb on + strand, within HP15_1719 at 1818.693 kb on + strand, within HP15_1719 at 1818.707 kb on - strand, within HP15_1719 at 1818.779 kb on + strand at 1818.792 kb on - strand at 1818.801 kb on + strand at 1818.801 kb on + strand at 1818.804 kb on - strand at 1818.814 kb on - strand at 1818.818 kb on + strand at 1818.828 kb on + strand at 1818.836 kb on - strand at 1818.926 kb on + strand, within HP15_1720 at 1819.004 kb on + strand at 1819.150 kb on - strand at 1819.175 kb on - strand, within HP15_1721 at 1819.292 kb on - strand, within HP15_1721 at 1819.329 kb on - strand, within HP15_1721 at 1819.454 kb on + strand, within HP15_1721 at 1819.637 kb on - strand, within HP15_1721 at 1819.769 kb on - strand, within HP15_1721 at 1819.880 kb on + strand, within HP15_1721 at 1819.897 kb on - strand, within HP15_1721 at 1819.999 kb on + strand at 1819.999 kb on + strand at 1819.999 kb on + strand at 1820.007 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine remove 1,817,892 + HP15_1718 0.78 -0.6 1,817,975 - HP15_1718 0.82 -0.8 1,818,304 + +0.0 1,818,334 - +0.9 1,818,691 - HP15_1719 0.73 +1.1 1,818,693 + HP15_1719 0.73 +0.3 1,818,693 + HP15_1719 0.73 -0.3 1,818,693 + HP15_1719 0.73 -0.7 1,818,707 - HP15_1719 0.76 +0.3 1,818,779 + -0.7 1,818,792 - -0.3 1,818,801 + -0.7 1,818,801 + +0.6 1,818,804 - +0.1 1,818,814 - -0.7 1,818,818 + -0.2 1,818,828 + +0.6 1,818,836 - -0.1 1,818,926 + HP15_1720 0.46 -0.6 1,819,004 + +0.3 1,819,150 - +1.6 1,819,175 - HP15_1721 0.12 +0.3 1,819,292 - HP15_1721 0.25 -0.2 1,819,329 - HP15_1721 0.28 +0.3 1,819,454 + HP15_1721 0.41 -1.1 1,819,637 - HP15_1721 0.61 -0.3 1,819,769 - HP15_1721 0.74 -0.4 1,819,880 + HP15_1721 0.86 -0.4 1,819,897 - HP15_1721 0.88 -0.4 1,819,999 + +0.9 1,819,999 + +0.3 1,819,999 + -0.6 1,820,007 - -0.2
Or see this region's nucleotide sequence