Experiment: D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_155 and HP15_156 are separated by 49 nucleotides HP15_156 and HP15_157 are separated by 389 nucleotides HP15_157 and HP15_158 are separated by 186 nucleotides HP15_158 and HP15_159 are separated by 60 nucleotides
HP15_155: HP15_155 - conserved hypothetical protein, membrane, at 154,953 to 155,630
_155
HP15_156: HP15_156 - conserved hypothetical protein, at 155,680 to 155,997
_156
HP15_157: HP15_157 - conserved hypothetical protein, at 156,387 to 156,779
_157
HP15_158: HP15_158 - protein MerR-like protein, at 156,966 to 157,226
_158
HP15_159: HP15_159 - membrane protein containing cytochrome c assembly protein, transmembrane region domain, at 157,287 to 158,009
_159
Position (kb) 156
157 Strain fitness (log2 ratio) -2
-1
0
1
2 at 155.519 kb on + strand, within HP15_155 at 155.519 kb on + strand, within HP15_155 at 155.519 kb on + strand, within HP15_155 at 155.534 kb on - strand, within HP15_155 at 155.538 kb on + strand, within HP15_155 at 155.546 kb on - strand, within HP15_155 at 155.546 kb on - strand, within HP15_155 at 155.571 kb on + strand at 155.571 kb on + strand at 155.579 kb on - strand at 155.598 kb on - strand at 155.598 kb on - strand at 155.630 kb on + strand at 155.639 kb on + strand at 155.639 kb on + strand at 155.649 kb on - strand at 155.683 kb on - strand at 155.688 kb on + strand at 155.698 kb on + strand at 155.788 kb on + strand, within HP15_156 at 155.882 kb on + strand, within HP15_156 at 155.884 kb on - strand, within HP15_156 at 155.884 kb on - strand, within HP15_156 at 155.999 kb on - strand at 155.999 kb on - strand at 156.004 kb on - strand at 156.009 kb on - strand at 156.022 kb on - strand at 156.048 kb on + strand at 156.156 kb on + strand at 156.156 kb on + strand at 156.187 kb on + strand at 156.280 kb on + strand at 156.280 kb on + strand at 156.288 kb on - strand at 156.316 kb on - strand at 156.343 kb on - strand at 156.381 kb on + strand at 156.671 kb on + strand, within HP15_157 at 156.729 kb on - strand, within HP15_157 at 156.729 kb on - strand, within HP15_157 at 156.731 kb on + strand, within HP15_157 at 156.739 kb on - strand, within HP15_157 at 156.741 kb on + strand at 156.749 kb on - strand at 156.824 kb on + strand at 156.845 kb on - strand at 156.949 kb on - strand at 156.949 kb on - strand at 157.120 kb on - strand, within HP15_158 at 157.120 kb on - strand, within HP15_158 at 157.122 kb on + strand, within HP15_158 at 157.312 kb on + strand at 157.421 kb on + strand, within HP15_159 at 157.458 kb on + strand, within HP15_159 at 157.549 kb on - strand, within HP15_159 at 157.556 kb on - strand, within HP15_159 at 157.571 kb on + strand, within HP15_159 at 157.571 kb on + strand, within HP15_159 at 157.627 kb on - strand, within HP15_159 at 157.627 kb on - strand, within HP15_159 at 157.644 kb on + strand, within HP15_159 at 157.704 kb on - strand, within HP15_159 at 157.713 kb on - strand, within HP15_159 at 157.760 kb on + strand, within HP15_159
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine remove 155,519 + HP15_155 0.83 -1.2 155,519 + HP15_155 0.83 -0.2 155,519 + HP15_155 0.83 +1.0 155,534 - HP15_155 0.86 -0.2 155,538 + HP15_155 0.86 -0.4 155,546 - HP15_155 0.87 -0.8 155,546 - HP15_155 0.87 -1.2 155,571 + +0.4 155,571 + +1.0 155,579 - -0.3 155,598 - -1.1 155,598 - -0.7 155,630 + -0.2 155,639 + +0.9 155,639 + +0.4 155,649 - +0.6 155,683 - +0.0 155,688 + -0.6 155,698 + +0.2 155,788 + HP15_156 0.34 -0.5 155,882 + HP15_156 0.64 +0.8 155,884 - HP15_156 0.64 -0.5 155,884 - HP15_156 0.64 -2.2 155,999 - +0.3 155,999 - -0.2 156,004 - +1.6 156,009 - +0.6 156,022 - +0.8 156,048 + +1.1 156,156 + -1.2 156,156 + +0.7 156,187 + +0.6 156,280 + -0.6 156,280 + +0.1 156,288 - -0.2 156,316 - +0.5 156,343 - +0.8 156,381 + +1.6 156,671 + HP15_157 0.72 +0.1 156,729 - HP15_157 0.87 +0.3 156,729 - HP15_157 0.87 +0.3 156,731 + HP15_157 0.88 +1.5 156,739 - HP15_157 0.90 -0.2 156,741 + +0.7 156,749 - -0.4 156,824 + -0.2 156,845 - -0.2 156,949 - -0.9 156,949 - -0.4 157,120 - HP15_158 0.59 +0.1 157,120 - HP15_158 0.59 +1.1 157,122 + HP15_158 0.60 +0.0 157,312 + -0.0 157,421 + HP15_159 0.19 -0.7 157,458 + HP15_159 0.24 +0.6 157,549 - HP15_159 0.36 -0.1 157,556 - HP15_159 0.37 +2.1 157,571 + HP15_159 0.39 +0.6 157,571 + HP15_159 0.39 -0.2 157,627 - HP15_159 0.47 -1.2 157,627 - HP15_159 0.47 -0.1 157,644 + HP15_159 0.49 -0.1 157,704 - HP15_159 0.58 +0.0 157,713 - HP15_159 0.59 +0.7 157,760 + HP15_159 0.65 +2.1
Or see this region's nucleotide sequence