Experiment: D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_999 and HP15_1000 are separated by 94 nucleotides HP15_1000 and HP15_1001 are separated by 14 nucleotides HP15_1001 and HP15_1002 overlap by 1 nucleotides
HP15_999: HP15_999 - acyl-CoA synthase, at 1,008,186 to 1,009,832
_999
HP15_1000: HP15_1000 - uncharacterized peroxidase-related protein, at 1,009,927 to 1,010,502
_1000
HP15_1001: HP15_1001 - pyruvate carboxyltransferase, at 1,010,517 to 1,011,422
_1001
HP15_1002: HP15_1002 - methylcrotonyl-CoA carboxylase, alpha subunit, at 1,011,422 to 1,013,416
_1002
Position (kb)
1009
1010
1011 Strain fitness (log2 ratio)
-1
0
1
2 at 1008.931 kb on - strand, within HP15_999 at 1008.938 kb on - strand, within HP15_999 at 1008.981 kb on + strand, within HP15_999 at 1008.989 kb on - strand, within HP15_999 at 1009.038 kb on + strand, within HP15_999 at 1009.070 kb on + strand, within HP15_999 at 1009.097 kb on - strand, within HP15_999 at 1009.120 kb on - strand, within HP15_999 at 1009.211 kb on - strand, within HP15_999 at 1009.211 kb on - strand, within HP15_999 at 1009.216 kb on - strand, within HP15_999 at 1009.240 kb on + strand, within HP15_999 at 1009.411 kb on + strand, within HP15_999 at 1009.419 kb on - strand, within HP15_999 at 1009.419 kb on - strand, within HP15_999 at 1009.499 kb on - strand, within HP15_999 at 1009.563 kb on - strand, within HP15_999 at 1009.686 kb on + strand at 1009.755 kb on + strand at 1009.755 kb on + strand at 1009.848 kb on + strand at 1009.872 kb on - strand at 1009.926 kb on - strand at 1009.985 kb on + strand, within HP15_1000 at 1010.119 kb on + strand, within HP15_1000 at 1010.179 kb on - strand, within HP15_1000 at 1010.361 kb on + strand, within HP15_1000
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine remove 1,008,931 - HP15_999 0.45 +0.8 1,008,938 - HP15_999 0.46 -1.0 1,008,981 + HP15_999 0.48 -0.1 1,008,989 - HP15_999 0.49 +1.7 1,009,038 + HP15_999 0.52 +0.2 1,009,070 + HP15_999 0.54 -0.1 1,009,097 - HP15_999 0.55 +1.7 1,009,120 - HP15_999 0.57 +2.0 1,009,211 - HP15_999 0.62 -0.2 1,009,211 - HP15_999 0.62 -0.3 1,009,216 - HP15_999 0.63 +0.9 1,009,240 + HP15_999 0.64 -0.6 1,009,411 + HP15_999 0.74 -0.5 1,009,419 - HP15_999 0.75 +0.2 1,009,419 - HP15_999 0.75 +0.5 1,009,499 - HP15_999 0.80 +1.2 1,009,563 - HP15_999 0.84 -0.4 1,009,686 + +0.1 1,009,755 + +0.1 1,009,755 + +0.0 1,009,848 + +0.0 1,009,872 - -0.8 1,009,926 - +0.7 1,009,985 + HP15_1000 0.10 -0.7 1,010,119 + HP15_1000 0.33 -0.2 1,010,179 - HP15_1000 0.44 -0.4 1,010,361 + HP15_1000 0.75 -0.5
Or see this region's nucleotide sequence