Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS24245

Experiment: L-Valine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS24235 and BWI76_RS24240 are separated by 117 nucleotidesBWI76_RS24240 and BWI76_RS24245 overlap by 59 nucleotidesBWI76_RS24245 and BWI76_RS24250 are separated by 204 nucleotidesBWI76_RS24250 and BWI76_RS24255 are separated by 60 nucleotides BWI76_RS24235: BWI76_RS24235 - Branched-chain-amino-acid transaminase (EC 2.6.1.42) (from data), at 5,050,849 to 5,052,030 _RS24235 BWI76_RS24240: BWI76_RS24240 - hypothetical protein, at 5,052,148 to 5,052,354 _RS24240 BWI76_RS24245: BWI76_RS24245 - bifunctional glutathionylspermidine amidase/glutathionylspermidine synthase, at 5,052,296 to 5,054,167 _RS24245 BWI76_RS24250: BWI76_RS24250 - hypothetical protein, at 5,054,372 to 5,054,932 _RS24250 BWI76_RS24255: BWI76_RS24255 - thiol:disulfide oxidoreductase, at 5,054,993 to 5,055,859 _RS24255 Position (kb) 5052 5053 5054 5055Strain fitness (log2 ratio) -2 -1 0 1 2at 5051.542 kb on - strand, within BWI76_RS24235at 5051.806 kb on - strand, within BWI76_RS24235at 5051.860 kb on + strand, within BWI76_RS24235at 5051.869 kb on + strand, within BWI76_RS24235at 5051.869 kb on - strand, within BWI76_RS24235at 5051.892 kb on - strand, within BWI76_RS24235at 5051.947 kb on + strandat 5051.959 kb on - strandat 5051.970 kb on - strandat 5051.987 kb on + strandat 5052.194 kb on + strand, within BWI76_RS24240at 5052.414 kb on + strandat 5052.453 kb on + strandat 5052.818 kb on - strand, within BWI76_RS24245at 5052.977 kb on - strand, within BWI76_RS24245at 5053.002 kb on + strand, within BWI76_RS24245at 5053.021 kb on + strand, within BWI76_RS24245at 5053.088 kb on + strand, within BWI76_RS24245at 5053.497 kb on + strand, within BWI76_RS24245at 5053.673 kb on - strand, within BWI76_RS24245at 5053.819 kb on - strand, within BWI76_RS24245at 5053.824 kb on - strand, within BWI76_RS24245at 5054.009 kb on + strandat 5054.117 kb on + strandat 5054.117 kb on + strandat 5054.125 kb on - strandat 5054.125 kb on - strandat 5054.274 kb on - strandat 5054.407 kb on + strandat 5054.415 kb on - strandat 5054.438 kb on - strand, within BWI76_RS24250at 5054.452 kb on + strand, within BWI76_RS24250at 5054.485 kb on + strand, within BWI76_RS24250at 5054.636 kb on - strand, within BWI76_RS24250at 5054.676 kb on + strand, within BWI76_RS24250at 5055.025 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Valine (N)
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5,051,542 - BWI76_RS24235 0.59 -2.4
5,051,806 - BWI76_RS24235 0.81 -1.2
5,051,860 + BWI76_RS24235 0.86 -0.4
5,051,869 + BWI76_RS24235 0.86 -0.9
5,051,869 - BWI76_RS24235 0.86 -0.3
5,051,892 - BWI76_RS24235 0.88 -1.6
5,051,947 + -1.7
5,051,959 - -1.5
5,051,970 - -1.5
5,051,987 + -1.2
5,052,194 + BWI76_RS24240 0.22 -1.2
5,052,414 + -0.6
5,052,453 + +0.9
5,052,818 - BWI76_RS24245 0.28 +1.9
5,052,977 - BWI76_RS24245 0.36 +0.2
5,053,002 + BWI76_RS24245 0.38 -0.9
5,053,021 + BWI76_RS24245 0.39 +1.9
5,053,088 + BWI76_RS24245 0.42 -1.0
5,053,497 + BWI76_RS24245 0.64 -0.3
5,053,673 - BWI76_RS24245 0.74 +2.1
5,053,819 - BWI76_RS24245 0.81 -1.3
5,053,824 - BWI76_RS24245 0.82 +0.4
5,054,009 + +0.1
5,054,117 + -1.7
5,054,117 + -1.0
5,054,125 - -0.3
5,054,125 - -1.0
5,054,274 - +1.7
5,054,407 + -0.5
5,054,415 - +1.2
5,054,438 - BWI76_RS24250 0.12 -0.1
5,054,452 + BWI76_RS24250 0.14 +0.2
5,054,485 + BWI76_RS24250 0.20 -0.3
5,054,636 - BWI76_RS24250 0.47 +0.9
5,054,676 + BWI76_RS24250 0.54 +0.3
5,055,025 - +0.2

Or see this region's nucleotide sequence