Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS06395

Experiment: L-Valine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS06385 and BWI76_RS06390 are separated by 0 nucleotidesBWI76_RS06390 and BWI76_RS06395 are separated by 198 nucleotidesBWI76_RS06395 and BWI76_RS06400 are separated by 58 nucleotidesBWI76_RS06400 and BWI76_RS06405 overlap by 38 nucleotides BWI76_RS06385: BWI76_RS06385 - (2E,6E)-farnesyl diphosphate synthase, at 1,342,490 to 1,343,389 _RS06385 BWI76_RS06390: BWI76_RS06390 - exodeoxyribonuclease VII small subunit, at 1,343,390 to 1,343,632 _RS06390 BWI76_RS06395: BWI76_RS06395 - tRNA 4-thiouridine(8) synthase ThiI, at 1,343,831 to 1,345,279 _RS06395 BWI76_RS06400: BWI76_RS06400 - oxidative-stress-resistance chaperone, at 1,345,338 to 1,345,928 _RS06400 BWI76_RS06405: BWI76_RS06405 - 2-dehydropantoate 2-reductase, at 1,345,891 to 1,346,802 _RS06405 Position (kb) 1343 1344 1345 1346Strain fitness (log2 ratio) -1 0 1at 1343.737 kb on + strandat 1343.754 kb on + strandat 1344.077 kb on + strand, within BWI76_RS06395at 1344.140 kb on + strand, within BWI76_RS06395at 1344.507 kb on + strand, within BWI76_RS06395at 1345.113 kb on - strand, within BWI76_RS06395at 1345.420 kb on - strand, within BWI76_RS06400at 1345.780 kb on - strandat 1345.835 kb on + strand, within BWI76_RS06400

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Valine (N)
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1,343,737 + +0.8
1,343,754 + +0.4
1,344,077 + BWI76_RS06395 0.17 -1.1
1,344,140 + BWI76_RS06395 0.21 -1.0
1,344,507 + BWI76_RS06395 0.47 -0.9
1,345,113 - BWI76_RS06395 0.88 +0.4
1,345,420 - BWI76_RS06400 0.14 -1.2
1,345,780 - +1.4
1,345,835 + BWI76_RS06400 0.84 -0.6

Or see this region's nucleotide sequence