Experiment: L-Valine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06385 and BWI76_RS06390 are separated by 0 nucleotides BWI76_RS06390 and BWI76_RS06395 are separated by 198 nucleotides BWI76_RS06395 and BWI76_RS06400 are separated by 58 nucleotides BWI76_RS06400 and BWI76_RS06405 overlap by 38 nucleotides
BWI76_RS06385: BWI76_RS06385 - (2E,6E)-farnesyl diphosphate synthase, at 1,342,490 to 1,343,389
_RS06385
BWI76_RS06390: BWI76_RS06390 - exodeoxyribonuclease VII small subunit, at 1,343,390 to 1,343,632
_RS06390
BWI76_RS06395: BWI76_RS06395 - tRNA 4-thiouridine(8) synthase ThiI, at 1,343,831 to 1,345,279
_RS06395
BWI76_RS06400: BWI76_RS06400 - oxidative-stress-resistance chaperone, at 1,345,338 to 1,345,928
_RS06400
BWI76_RS06405: BWI76_RS06405 - 2-dehydropantoate 2-reductase, at 1,345,891 to 1,346,802
_RS06405
Position (kb)
1343
1344
1345
1346 Strain fitness (log2 ratio)
-1
0
1 at 1343.737 kb on + strand at 1343.754 kb on + strand at 1344.077 kb on + strand, within BWI76_RS06395 at 1344.140 kb on + strand, within BWI76_RS06395 at 1344.507 kb on + strand, within BWI76_RS06395 at 1345.113 kb on - strand, within BWI76_RS06395 at 1345.420 kb on - strand, within BWI76_RS06400 at 1345.780 kb on - strand at 1345.835 kb on + strand, within BWI76_RS06400
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (N) remove 1,343,737 + +0.8 1,343,754 + +0.4 1,344,077 + BWI76_RS06395 0.17 -1.1 1,344,140 + BWI76_RS06395 0.21 -1.0 1,344,507 + BWI76_RS06395 0.47 -0.9 1,345,113 - BWI76_RS06395 0.88 +0.4 1,345,420 - BWI76_RS06400 0.14 -1.2 1,345,780 - +1.4 1,345,835 + BWI76_RS06400 0.84 -0.6
Or see this region's nucleotide sequence