Experiment: L-Valine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00835 and BWI76_RS00840 are separated by 254 nucleotides BWI76_RS00840 and BWI76_RS00845 are separated by 184 nucleotides
BWI76_RS00835: BWI76_RS00835 - phosphoenolpyruvate carboxylase, at 170,679 to 173,330
_RS00835
BWI76_RS00840: BWI76_RS00840 - acetylornithine deacetylase, at 173,585 to 174,736
_RS00840
BWI76_RS00845: BWI76_RS00845 - N-acetyl-gamma-glutamyl-phosphate reductase, at 174,921 to 175,925
_RS00845
Position (kb) 173
174
175 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 172.641 kb on + strand, within BWI76_RS00835 at 172.692 kb on - strand, within BWI76_RS00835 at 172.853 kb on + strand, within BWI76_RS00835 at 172.853 kb on + strand, within BWI76_RS00835 at 172.861 kb on - strand, within BWI76_RS00835 at 172.861 kb on - strand, within BWI76_RS00835 at 172.928 kb on + strand, within BWI76_RS00835 at 173.110 kb on - strand at 173.127 kb on - strand at 173.311 kb on + strand at 173.334 kb on + strand at 173.342 kb on - strand at 173.539 kb on + strand at 173.619 kb on - strand at 173.698 kb on - strand at 173.737 kb on + strand, within BWI76_RS00840 at 173.863 kb on + strand, within BWI76_RS00840 at 173.960 kb on - strand, within BWI76_RS00840 at 173.960 kb on - strand, within BWI76_RS00840 at 174.257 kb on + strand, within BWI76_RS00840 at 174.352 kb on + strand, within BWI76_RS00840 at 174.432 kb on - strand, within BWI76_RS00840 at 174.432 kb on - strand, within BWI76_RS00840 at 174.445 kb on + strand, within BWI76_RS00840 at 174.445 kb on + strand, within BWI76_RS00840 at 174.453 kb on - strand, within BWI76_RS00840 at 174.497 kb on - strand, within BWI76_RS00840 at 174.674 kb on + strand at 174.707 kb on - strand at 174.966 kb on + strand at 174.996 kb on + strand at 175.207 kb on + strand, within BWI76_RS00845 at 175.291 kb on + strand, within BWI76_RS00845 at 175.291 kb on + strand, within BWI76_RS00845 at 175.329 kb on - strand, within BWI76_RS00845 at 175.483 kb on - strand, within BWI76_RS00845 at 175.498 kb on - strand, within BWI76_RS00845 at 175.593 kb on + strand, within BWI76_RS00845 at 175.709 kb on - strand, within BWI76_RS00845 at 175.714 kb on - strand, within BWI76_RS00845
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (N) remove 172,641 + BWI76_RS00835 0.74 -2.3 172,692 - BWI76_RS00835 0.76 -2.9 172,853 + BWI76_RS00835 0.82 -3.2 172,853 + BWI76_RS00835 0.82 -2.7 172,861 - BWI76_RS00835 0.82 -5.0 172,861 - BWI76_RS00835 0.82 -1.4 172,928 + BWI76_RS00835 0.85 -3.3 173,110 - -2.8 173,127 - -3.2 173,311 + -1.3 173,334 + -2.6 173,342 - +0.3 173,539 + +0.0 173,619 - -1.4 173,698 - +2.4 173,737 + BWI76_RS00840 0.13 -2.5 173,863 + BWI76_RS00840 0.24 -0.5 173,960 - BWI76_RS00840 0.33 -1.7 173,960 - BWI76_RS00840 0.33 +0.9 174,257 + BWI76_RS00840 0.58 -1.1 174,352 + BWI76_RS00840 0.67 -0.5 174,432 - BWI76_RS00840 0.74 -1.0 174,432 - BWI76_RS00840 0.74 -1.0 174,445 + BWI76_RS00840 0.75 -0.8 174,445 + BWI76_RS00840 0.75 -1.3 174,453 - BWI76_RS00840 0.75 -1.2 174,497 - BWI76_RS00840 0.79 -0.6 174,674 + +0.1 174,707 - -1.2 174,966 + -3.3 174,996 + -2.4 175,207 + BWI76_RS00845 0.28 -1.5 175,291 + BWI76_RS00845 0.37 -1.8 175,291 + BWI76_RS00845 0.37 +1.0 175,329 - BWI76_RS00845 0.41 -1.7 175,483 - BWI76_RS00845 0.56 -2.5 175,498 - BWI76_RS00845 0.57 -1.8 175,593 + BWI76_RS00845 0.67 -2.5 175,709 - BWI76_RS00845 0.78 -3.7 175,714 - BWI76_RS00845 0.79 -2.8
Or see this region's nucleotide sequence