Experiment: Formate/Acetate-Sulfite (60/30-40 mM) + 0.1% Yeast Extract
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phsA and DVU0174 are separated by 82 nucleotides DVU0174 and DVU0175 are separated by 476 nucleotides DVU0175 and DVU0176 are separated by 255 nucleotides
DVU0173: phsA - thiosulfate reductase (Shelley Haveman), at 216,495 to 218,591
phsA
DVU0174: DVU0174 - hypothetical protein (TIGR), at 218,674 to 218,781
DVU0174
DVU0175: DVU0175 - tungsten formylmethanofuran dehydrogenase family protein/molybdopterin binding protein (TIGR), at 219,258 to 220,889
DVU0175
DVU0176: DVU0176 - glycerophosphoryl diester phosphodiesterase family protein (TIGR), at 221,145 to 222,044
DVU0176
Position (kb)
219
220
221 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 218.262 kb on + strand, within phsA at 218.389 kb on + strand at 218.468 kb on - strand at 218.477 kb on - strand at 218.485 kb on + strand at 218.493 kb on - strand at 218.524 kb on - strand at 218.531 kb on + strand at 218.539 kb on - strand at 218.539 kb on - strand at 218.607 kb on + strand at 218.609 kb on + strand at 218.617 kb on - strand at 218.639 kb on + strand at 218.648 kb on + strand at 218.681 kb on - strand at 218.683 kb on + strand at 218.688 kb on + strand, within DVU0174 at 218.693 kb on + strand, within DVU0174 at 218.696 kb on - strand, within DVU0174 at 218.698 kb on + strand, within DVU0174 at 218.698 kb on + strand, within DVU0174 at 218.713 kb on - strand, within DVU0174 at 218.933 kb on + strand at 218.948 kb on + strand at 218.953 kb on + strand at 218.953 kb on + strand at 218.961 kb on - strand at 218.997 kb on + strand at 219.000 kb on - strand at 219.035 kb on - strand at 219.037 kb on - strand at 219.038 kb on + strand at 219.130 kb on + strand at 219.264 kb on - strand at 219.264 kb on - strand at 219.264 kb on - strand at 219.264 kb on - strand at 219.279 kb on + strand at 219.330 kb on - strand at 219.338 kb on + strand at 219.386 kb on + strand at 219.387 kb on + strand at 219.395 kb on - strand at 219.430 kb on + strand, within DVU0175 at 219.430 kb on + strand, within DVU0175 at 219.438 kb on - strand, within DVU0175 at 219.472 kb on + strand, within DVU0175 at 219.472 kb on + strand, within DVU0175 at 219.512 kb on + strand, within DVU0175 at 219.582 kb on - strand, within DVU0175 at 219.651 kb on - strand, within DVU0175 at 219.688 kb on - strand, within DVU0175 at 219.727 kb on + strand, within DVU0175 at 219.787 kb on + strand, within DVU0175 at 219.790 kb on - strand, within DVU0175 at 219.805 kb on + strand, within DVU0175 at 219.805 kb on + strand, within DVU0175 at 219.805 kb on + strand, within DVU0175 at 219.813 kb on - strand, within DVU0175 at 219.841 kb on - strand, within DVU0175 at 219.852 kb on - strand, within DVU0175 at 219.948 kb on - strand, within DVU0175 at 219.948 kb on - strand, within DVU0175 at 219.948 kb on - strand, within DVU0175 at 219.950 kb on + strand, within DVU0175 at 219.950 kb on + strand, within DVU0175 at 219.958 kb on - strand, within DVU0175 at 219.958 kb on - strand, within DVU0175 at 219.984 kb on + strand, within DVU0175 at 220.050 kb on + strand, within DVU0175 at 220.058 kb on - strand, within DVU0175 at 220.058 kb on - strand, within DVU0175 at 220.098 kb on + strand, within DVU0175 at 220.099 kb on - strand, within DVU0175 at 220.127 kb on + strand, within DVU0175 at 220.127 kb on + strand, within DVU0175 at 220.133 kb on - strand, within DVU0175 at 220.133 kb on - strand, within DVU0175 at 220.190 kb on - strand, within DVU0175 at 220.208 kb on - strand, within DVU0175 at 220.215 kb on - strand, within DVU0175 at 220.296 kb on - strand, within DVU0175 at 220.313 kb on + strand, within DVU0175 at 220.460 kb on - strand, within DVU0175 at 220.479 kb on + strand, within DVU0175 at 220.545 kb on - strand, within DVU0175 at 220.545 kb on - strand, within DVU0175 at 220.599 kb on + strand, within DVU0175 at 220.637 kb on + strand, within DVU0175 at 220.637 kb on + strand, within DVU0175 at 220.725 kb on + strand, within DVU0175 at 220.793 kb on + strand at 220.798 kb on - strand at 220.902 kb on - strand at 221.015 kb on - strand at 221.065 kb on + strand at 221.239 kb on + strand, within DVU0176 at 221.356 kb on + strand, within DVU0176 at 221.397 kb on - strand, within DVU0176 at 221.492 kb on + strand, within DVU0176 at 221.497 kb on + strand, within DVU0176 at 221.502 kb on + strand, within DVU0176 at 221.510 kb on - strand, within DVU0176 at 221.553 kb on + strand, within DVU0176 at 221.629 kb on + strand, within DVU0176 at 221.660 kb on - strand, within DVU0176 at 221.662 kb on + strand, within DVU0176 at 221.670 kb on - strand, within DVU0176 at 221.779 kb on - strand, within DVU0176 at 221.816 kb on + strand, within DVU0176
Per-strain Table
Position Strand Gene LocusTag Fraction Formate/Acetate-Sulfite (60/30-40 mM) + 0.1% Yeast Extract remove 218,262 + phsA DVU0173 0.84 -0.7 218,389 + -0.4 218,468 - -0.7 218,477 - -0.5 218,485 + -1.6 218,493 - -0.7 218,524 - -0.1 218,531 + -1.0 218,539 - -1.5 218,539 - -0.6 218,607 + +0.4 218,609 + -0.1 218,617 - -1.7 218,639 + -0.6 218,648 + -0.7 218,681 - -0.2 218,683 + -1.2 218,688 + DVU0174 0.13 +1.4 218,693 + DVU0174 0.18 +2.4 218,696 - DVU0174 0.20 -0.1 218,698 + DVU0174 0.22 +0.4 218,698 + DVU0174 0.22 +1.1 218,713 - DVU0174 0.36 -1.0 218,933 + -1.4 218,948 + -0.3 218,953 + -0.2 218,953 + +0.1 218,961 - +0.1 218,997 + +0.3 219,000 - +0.2 219,035 - +0.4 219,037 - -0.7 219,038 + -0.1 219,130 + -0.4 219,264 - -0.8 219,264 - -0.4 219,264 - -0.5 219,264 - +0.4 219,279 + -1.9 219,330 - -1.1 219,338 + -0.6 219,386 + -0.6 219,387 + -0.8 219,395 - -1.2 219,430 + DVU0175 0.11 -0.6 219,430 + DVU0175 0.11 -0.2 219,438 - DVU0175 0.11 -1.6 219,472 + DVU0175 0.13 -0.1 219,472 + DVU0175 0.13 -0.5 219,512 + DVU0175 0.16 +0.2 219,582 - DVU0175 0.20 -1.2 219,651 - DVU0175 0.24 -0.6 219,688 - DVU0175 0.26 -0.9 219,727 + DVU0175 0.29 -1.3 219,787 + DVU0175 0.32 +0.4 219,790 - DVU0175 0.33 -0.6 219,805 + DVU0175 0.34 -0.5 219,805 + DVU0175 0.34 -0.2 219,805 + DVU0175 0.34 -2.2 219,813 - DVU0175 0.34 -0.8 219,841 - DVU0175 0.36 +1.4 219,852 - DVU0175 0.36 -0.9 219,948 - DVU0175 0.42 -1.2 219,948 - DVU0175 0.42 -0.2 219,948 - DVU0175 0.42 -1.0 219,950 + DVU0175 0.42 -0.5 219,950 + DVU0175 0.42 -0.5 219,958 - DVU0175 0.43 -1.9 219,958 - DVU0175 0.43 -1.6 219,984 + DVU0175 0.44 -1.2 220,050 + DVU0175 0.49 -1.5 220,058 - DVU0175 0.49 -0.6 220,058 - DVU0175 0.49 -0.6 220,098 + DVU0175 0.51 -0.9 220,099 - DVU0175 0.52 -1.0 220,127 + DVU0175 0.53 -0.6 220,127 + DVU0175 0.53 -0.6 220,133 - DVU0175 0.54 -0.6 220,133 - DVU0175 0.54 -0.9 220,190 - DVU0175 0.57 -1.8 220,208 - DVU0175 0.58 -0.8 220,215 - DVU0175 0.59 -0.2 220,296 - DVU0175 0.64 -1.9 220,313 + DVU0175 0.65 -0.1 220,460 - DVU0175 0.74 +1.0 220,479 + DVU0175 0.75 -0.6 220,545 - DVU0175 0.79 -0.4 220,545 - DVU0175 0.79 -1.2 220,599 + DVU0175 0.82 -0.6 220,637 + DVU0175 0.84 +0.4 220,637 + DVU0175 0.84 -0.9 220,725 + DVU0175 0.90 -0.1 220,793 + -2.2 220,798 - -1.0 220,902 - -0.6 221,015 - +0.3 221,065 + -3.2 221,239 + DVU0176 0.10 -0.3 221,356 + DVU0176 0.23 -0.6 221,397 - DVU0176 0.28 -0.0 221,492 + DVU0176 0.39 -0.3 221,497 + DVU0176 0.39 -1.9 221,502 + DVU0176 0.40 -0.6 221,510 - DVU0176 0.41 -0.0 221,553 + DVU0176 0.45 -1.6 221,629 + DVU0176 0.54 +0.4 221,660 - DVU0176 0.57 -0.6 221,662 + DVU0176 0.57 -0.2 221,670 - DVU0176 0.58 -1.0 221,779 - DVU0176 0.70 +0.7 221,816 + DVU0176 0.75 +0.3
Or see this region's nucleotide sequence