Experiment: 3-hydroxybutyric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02968 and H281DRAFT_02969 are separated by 11 nucleotides H281DRAFT_02969 and H281DRAFT_02970 overlap by 4 nucleotides H281DRAFT_02970 and H281DRAFT_02971 overlap by 4 nucleotides H281DRAFT_02971 and H281DRAFT_02972 are separated by 146 nucleotides
H281DRAFT_02968: H281DRAFT_02968 - amidase, at 582,958 to 584,109
_02968
H281DRAFT_02969: H281DRAFT_02969 - Protein of unknown function (DUF4089), at 584,121 to 584,312
_02969
H281DRAFT_02970: H281DRAFT_02970 - aspartyl-tRNA(Asn)/glutamyl-tRNA(Gln) amidotransferase subunit A, at 584,309 to 585,730
_02970
H281DRAFT_02971: H281DRAFT_02971 - Protein of unknown function (DUF3225), at 585,727 to 586,107
_02971
H281DRAFT_02972: H281DRAFT_02972 - hypothetical protein, at 586,254 to 586,850
_02972
Position (kb)
584
585
586 Strain fitness (log2 ratio)
-1
0
1 at 583.394 kb on - strand, within H281DRAFT_02968 at 584.705 kb on + strand, within H281DRAFT_02970 at 584.904 kb on - strand, within H281DRAFT_02970 at 584.999 kb on + strand, within H281DRAFT_02970 at 585.107 kb on + strand, within H281DRAFT_02970 at 585.410 kb on + strand, within H281DRAFT_02970 at 585.964 kb on - strand, within H281DRAFT_02971 at 585.967 kb on + strand, within H281DRAFT_02971 at 586.256 kb on - strand at 586.372 kb on + strand, within H281DRAFT_02972 at 586.372 kb on + strand, within H281DRAFT_02972 at 586.477 kb on - strand, within H281DRAFT_02972
Per-strain Table
Position Strand Gene LocusTag Fraction 3-hydroxybutyric (C) remove 583,394 - H281DRAFT_02968 0.38 +0.1 584,705 + H281DRAFT_02970 0.28 +0.4 584,904 - H281DRAFT_02970 0.42 +0.5 584,999 + H281DRAFT_02970 0.49 +0.6 585,107 + H281DRAFT_02970 0.56 +0.3 585,410 + H281DRAFT_02970 0.77 +0.2 585,964 - H281DRAFT_02971 0.62 +0.4 585,967 + H281DRAFT_02971 0.63 +1.5 586,256 - +0.6 586,372 + H281DRAFT_02972 0.20 -0.5 586,372 + H281DRAFT_02972 0.20 -0.7 586,477 - H281DRAFT_02972 0.37 +0.8
Or see this region's nucleotide sequence