Experiment: m.b. Benzalkonium Chloride 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09130 and PGA1_c09140 overlap by 4 nucleotides PGA1_c09140 and PGA1_c09150 overlap by 4 nucleotides PGA1_c09150 and PGA1_c09160 are separated by 3 nucleotides PGA1_c09160 and PGA1_c09170 overlap by 4 nucleotides
PGA1_c09130: PGA1_c09130 - 8-amino-7-oxononanoate synthase BioF, at 938,722 to 939,861
_c09130
PGA1_c09140: PGA1_c09140 - dethiobiotin synthetase BioD, at 939,858 to 940,499
_c09140
PGA1_c09150: PGA1_c09150 - adenosylmethionine-8-amino-7-oxononanoate aminotransferase BioA, at 940,496 to 941,794
_c09150
PGA1_c09160: PGA1_c09160 - Uncharacterized protein conserved in bacteria, at 941,798 to 942,418
_c09160
PGA1_c09170: PGA1_c09170 - putative biotin synthesis protein BioC, at 942,415 to 943,203
_c09170
Position (kb) 940
941
942 Strain fitness (log2 ratio) -2
-1
0
1
2 at 939.528 kb on + strand, within PGA1_c09130 at 939.533 kb on + strand, within PGA1_c09130 at 939.541 kb on - strand, within PGA1_c09130 at 939.604 kb on - strand, within PGA1_c09130 at 939.634 kb on + strand, within PGA1_c09130 at 939.646 kb on + strand, within PGA1_c09130 at 939.661 kb on + strand, within PGA1_c09130 at 939.696 kb on + strand, within PGA1_c09130 at 939.696 kb on + strand, within PGA1_c09130 at 939.696 kb on + strand, within PGA1_c09130 at 939.696 kb on + strand, within PGA1_c09130 at 939.696 kb on + strand, within PGA1_c09130 at 939.704 kb on - strand, within PGA1_c09130 at 939.719 kb on + strand, within PGA1_c09130 at 939.727 kb on - strand, within PGA1_c09130 at 939.807 kb on + strand at 939.807 kb on + strand at 939.812 kb on + strand at 939.815 kb on - strand at 939.820 kb on - strand at 939.829 kb on + strand at 939.945 kb on - strand, within PGA1_c09140 at 939.997 kb on + strand, within PGA1_c09140 at 940.023 kb on - strand, within PGA1_c09140 at 940.033 kb on - strand, within PGA1_c09140 at 940.084 kb on - strand, within PGA1_c09140 at 940.156 kb on + strand, within PGA1_c09140 at 940.252 kb on + strand, within PGA1_c09140 at 940.300 kb on + strand, within PGA1_c09140 at 940.308 kb on - strand, within PGA1_c09140 at 940.311 kb on + strand, within PGA1_c09140 at 940.362 kb on - strand, within PGA1_c09140 at 940.458 kb on + strand at 940.486 kb on - strand at 940.486 kb on - strand at 940.511 kb on - strand at 940.549 kb on + strand at 940.549 kb on + strand at 940.549 kb on + strand at 940.557 kb on - strand at 940.557 kb on - strand at 940.557 kb on - strand at 940.621 kb on + strand at 940.657 kb on - strand, within PGA1_c09150 at 940.725 kb on - strand, within PGA1_c09150 at 940.730 kb on - strand, within PGA1_c09150 at 940.921 kb on - strand, within PGA1_c09150 at 941.007 kb on - strand, within PGA1_c09150 at 941.007 kb on - strand, within PGA1_c09150 at 941.125 kb on + strand, within PGA1_c09150 at 941.125 kb on + strand, within PGA1_c09150 at 941.159 kb on + strand, within PGA1_c09150 at 941.159 kb on + strand, within PGA1_c09150 at 941.164 kb on + strand, within PGA1_c09150 at 941.410 kb on - strand, within PGA1_c09150 at 941.427 kb on + strand, within PGA1_c09150 at 941.472 kb on - strand, within PGA1_c09150 at 941.482 kb on + strand, within PGA1_c09150 at 941.484 kb on - strand, within PGA1_c09150 at 941.504 kb on + strand, within PGA1_c09150 at 941.512 kb on - strand, within PGA1_c09150 at 941.525 kb on + strand, within PGA1_c09150 at 941.525 kb on + strand, within PGA1_c09150 at 941.528 kb on - strand, within PGA1_c09150 at 941.545 kb on + strand, within PGA1_c09150 at 941.545 kb on + strand, within PGA1_c09150 at 941.553 kb on - strand, within PGA1_c09150 at 941.559 kb on - strand, within PGA1_c09150 at 941.610 kb on + strand, within PGA1_c09150 at 941.618 kb on - strand, within PGA1_c09150 at 941.618 kb on - strand, within PGA1_c09150 at 941.707 kb on + strand at 941.715 kb on - strand at 941.715 kb on - strand at 941.758 kb on - strand at 941.788 kb on + strand at 941.814 kb on - strand at 941.820 kb on - strand at 941.825 kb on - strand at 941.931 kb on + strand, within PGA1_c09160 at 941.986 kb on - strand, within PGA1_c09160 at 942.013 kb on - strand, within PGA1_c09160 at 942.077 kb on - strand, within PGA1_c09160 at 942.077 kb on - strand, within PGA1_c09160 at 942.115 kb on + strand, within PGA1_c09160 at 942.130 kb on - strand, within PGA1_c09160 at 942.242 kb on - strand, within PGA1_c09160 at 942.249 kb on + strand, within PGA1_c09160 at 942.277 kb on + strand, within PGA1_c09160 at 942.277 kb on + strand, within PGA1_c09160 at 942.277 kb on + strand, within PGA1_c09160 at 942.285 kb on - strand, within PGA1_c09160 at 942.285 kb on - strand, within PGA1_c09160 at 942.285 kb on - strand, within PGA1_c09160 at 942.358 kb on + strand at 942.396 kb on + strand at 942.409 kb on + strand at 942.428 kb on - strand at 942.438 kb on - strand at 942.483 kb on - strand at 942.755 kb on - strand, within PGA1_c09170 at 942.756 kb on + strand, within PGA1_c09170 at 942.764 kb on + strand, within PGA1_c09170
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Benzalkonium Chloride 0.004 mg/ml remove 939,528 + PGA1_c09130 0.71 +0.2 939,533 + PGA1_c09130 0.71 +0.9 939,541 - PGA1_c09130 0.72 +0.0 939,604 - PGA1_c09130 0.77 -0.6 939,634 + PGA1_c09130 0.80 -1.0 939,646 + PGA1_c09130 0.81 +0.4 939,661 + PGA1_c09130 0.82 +0.2 939,696 + PGA1_c09130 0.85 -1.5 939,696 + PGA1_c09130 0.85 -0.5 939,696 + PGA1_c09130 0.85 +1.2 939,696 + PGA1_c09130 0.85 -1.1 939,696 + PGA1_c09130 0.85 +0.4 939,704 - PGA1_c09130 0.86 +0.4 939,719 + PGA1_c09130 0.87 -0.2 939,727 - PGA1_c09130 0.88 +1.1 939,807 + -0.9 939,807 + +0.2 939,812 + -0.1 939,815 - +0.4 939,820 - -0.6 939,829 + -0.3 939,945 - PGA1_c09140 0.14 -0.5 939,997 + PGA1_c09140 0.22 -2.1 940,023 - PGA1_c09140 0.26 +0.7 940,033 - PGA1_c09140 0.27 +0.9 940,084 - PGA1_c09140 0.35 -1.1 940,156 + PGA1_c09140 0.46 +0.7 940,252 + PGA1_c09140 0.61 +0.4 940,300 + PGA1_c09140 0.69 -0.6 940,308 - PGA1_c09140 0.70 -1.9 940,311 + PGA1_c09140 0.71 -0.8 940,362 - PGA1_c09140 0.79 +1.0 940,458 + +0.4 940,486 - -1.7 940,486 - -0.2 940,511 - +1.3 940,549 + +0.5 940,549 + +0.4 940,549 + +1.5 940,557 - +0.6 940,557 - +0.0 940,557 - -0.9 940,621 + +0.5 940,657 - PGA1_c09150 0.12 -0.6 940,725 - PGA1_c09150 0.18 -2.1 940,730 - PGA1_c09150 0.18 -1.5 940,921 - PGA1_c09150 0.33 +1.9 941,007 - PGA1_c09150 0.39 -0.6 941,007 - PGA1_c09150 0.39 +2.0 941,125 + PGA1_c09150 0.48 -0.7 941,125 + PGA1_c09150 0.48 +1.0 941,159 + PGA1_c09150 0.51 -0.4 941,159 + PGA1_c09150 0.51 -0.5 941,164 + PGA1_c09150 0.51 -0.2 941,410 - PGA1_c09150 0.70 -0.7 941,427 + PGA1_c09150 0.72 +0.9 941,472 - PGA1_c09150 0.75 +0.4 941,482 + PGA1_c09150 0.76 +0.2 941,484 - PGA1_c09150 0.76 -2.1 941,504 + PGA1_c09150 0.78 +1.4 941,512 - PGA1_c09150 0.78 +0.5 941,525 + PGA1_c09150 0.79 +0.1 941,525 + PGA1_c09150 0.79 -1.1 941,528 - PGA1_c09150 0.79 -0.6 941,545 + PGA1_c09150 0.81 +0.2 941,545 + PGA1_c09150 0.81 +2.5 941,553 - PGA1_c09150 0.81 -0.7 941,559 - PGA1_c09150 0.82 +0.8 941,610 + PGA1_c09150 0.86 -0.8 941,618 - PGA1_c09150 0.86 -2.3 941,618 - PGA1_c09150 0.86 +0.4 941,707 + +1.2 941,715 - -0.1 941,715 - +0.9 941,758 - -0.2 941,788 + +0.0 941,814 - -1.9 941,820 - -1.9 941,825 - +0.0 941,931 + PGA1_c09160 0.21 +1.0 941,986 - PGA1_c09160 0.30 -1.3 942,013 - PGA1_c09160 0.35 +1.3 942,077 - PGA1_c09160 0.45 -0.1 942,077 - PGA1_c09160 0.45 -0.9 942,115 + PGA1_c09160 0.51 +0.1 942,130 - PGA1_c09160 0.53 -0.1 942,242 - PGA1_c09160 0.71 +0.1 942,249 + PGA1_c09160 0.73 -0.1 942,277 + PGA1_c09160 0.77 +0.9 942,277 + PGA1_c09160 0.77 +0.2 942,277 + PGA1_c09160 0.77 -0.3 942,285 - PGA1_c09160 0.78 -1.1 942,285 - PGA1_c09160 0.78 -1.5 942,285 - PGA1_c09160 0.78 -1.5 942,358 + +1.8 942,396 + +0.9 942,409 + -0.4 942,428 - +1.3 942,438 - -1.6 942,483 - +0.7 942,755 - PGA1_c09170 0.43 +2.0 942,756 + PGA1_c09170 0.43 -0.5 942,764 + PGA1_c09170 0.44 -0.1
Or see this region's nucleotide sequence