Experiment: L-Phenylalanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_496 and HP15_497 overlap by 4 nucleotides HP15_497 and HP15_498 are separated by 17 nucleotides HP15_498 and HP15_499 are separated by 138 nucleotides HP15_499 and HP15_500 are separated by 110 nucleotides HP15_500 and HP15_501 are separated by 33 nucleotides
HP15_496: HP15_496 - thiamine-monophosphate kinase, at 510,398 to 511,354
_496
HP15_497: HP15_497 - phosphatidylglycerophosphatase A, at 511,351 to 511,878
_497
HP15_498: HP15_498 - thiolase-like protein, at 511,896 to 512,099
_498
HP15_499: HP15_499 - hypothetical protein, at 512,238 to 512,348
_499
HP15_500: HP15_500 - conserved hypothetical protein, at 512,459 to 513,064
_500
HP15_501: HP15_501 - acyltransferase, WS/DGAT/MGAT, at 513,098 to 514,684
_501
Position (kb) 511
512
513 Strain fitness (log2 ratio) -1
0
1
2 at 511.998 kb on - strand, within HP15_498 at 512.000 kb on - strand, within HP15_498 at 512.000 kb on - strand, within HP15_498 at 512.028 kb on + strand, within HP15_498 at 512.113 kb on - strand at 512.376 kb on + strand at 512.455 kb on - strand at 512.551 kb on + strand, within HP15_500 at 512.551 kb on - strand, within HP15_500 at 512.635 kb on - strand, within HP15_500 at 512.785 kb on - strand, within HP15_500 at 512.985 kb on + strand, within HP15_500 at 512.985 kb on + strand, within HP15_500 at 513.003 kb on - strand, within HP15_500 at 513.073 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine remove 511,998 - HP15_498 0.50 -0.8 512,000 - HP15_498 0.51 -1.0 512,000 - HP15_498 0.51 +0.0 512,028 + HP15_498 0.65 -1.3 512,113 - +1.9 512,376 + -0.0 512,455 - +1.3 512,551 + HP15_500 0.15 +1.3 512,551 - HP15_500 0.15 -0.4 512,635 - HP15_500 0.29 +0.7 512,785 - HP15_500 0.54 -0.4 512,985 + HP15_500 0.87 -1.3 512,985 + HP15_500 0.87 +1.0 513,003 - HP15_500 0.90 +0.6 513,073 - -0.4
Or see this region's nucleotide sequence