Experiment: reconstituted MRS with Vitagos-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13505 and BBR_RS13510 are separated by 17 nucleotides BBR_RS13510 and BBR_RS13515 are separated by 114 nucleotides BBR_RS13515 and BBR_RS13520 are separated by 84 nucleotides BBR_RS13520 and BBR_RS13525 are separated by 80 nucleotides
BBR_RS13505: BBR_RS13505 - DUF4391 domain-containing protein, at 803,720 to 804,451
_RS13505
BBR_RS13510: BBR_RS13510 - LysR family transcriptional regulator, at 804,469 to 805,284
_RS13510
BBR_RS13515: BBR_RS13515 - putative sulfate exporter family transporter, at 805,399 to 806,463
_RS13515
BBR_RS13520: BBR_RS13520 - polysaccharide synthesis protein GtrA, at 806,548 to 807,035
_RS13520
BBR_RS13525: BBR_RS13525 - CrcB family protein, at 807,116 to 807,481
_RS13525
Position (kb) 805
806
807 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 804.439 kb on - strand at 804.446 kb on + strand at 804.446 kb on + strand at 804.446 kb on + strand at 804.446 kb on + strand at 804.446 kb on + strand at 804.446 kb on + strand at 804.446 kb on + strand at 804.454 kb on - strand at 804.454 kb on - strand at 804.459 kb on + strand at 804.467 kb on - strand at 804.488 kb on - strand at 804.499 kb on + strand at 804.506 kb on + strand at 804.509 kb on - strand at 804.516 kb on + strand at 804.516 kb on + strand at 804.596 kb on + strand, within BBR_RS13510 at 804.599 kb on - strand, within BBR_RS13510 at 804.630 kb on + strand, within BBR_RS13510 at 804.653 kb on - strand, within BBR_RS13510 at 804.783 kb on - strand, within BBR_RS13510 at 804.876 kb on + strand, within BBR_RS13510 at 804.876 kb on + strand, within BBR_RS13510 at 804.884 kb on - strand, within BBR_RS13510 at 804.889 kb on + strand, within BBR_RS13510 at 804.935 kb on + strand, within BBR_RS13510 at 804.935 kb on + strand, within BBR_RS13510 at 804.935 kb on + strand, within BBR_RS13510 at 804.943 kb on - strand, within BBR_RS13510 at 804.943 kb on - strand, within BBR_RS13510 at 804.943 kb on - strand, within BBR_RS13510 at 804.954 kb on - strand, within BBR_RS13510 at 804.956 kb on + strand, within BBR_RS13510 at 804.991 kb on - strand, within BBR_RS13510 at 805.007 kb on + strand, within BBR_RS13510 at 805.007 kb on + strand, within BBR_RS13510 at 805.028 kb on - strand, within BBR_RS13510 at 805.056 kb on - strand, within BBR_RS13510 at 805.061 kb on - strand, within BBR_RS13510 at 805.078 kb on - strand, within BBR_RS13510 at 805.080 kb on + strand, within BBR_RS13510 at 805.126 kb on - strand, within BBR_RS13510 at 805.126 kb on - strand, within BBR_RS13510 at 805.152 kb on + strand, within BBR_RS13510 at 805.152 kb on + strand, within BBR_RS13510 at 805.154 kb on + strand, within BBR_RS13510 at 805.162 kb on - strand, within BBR_RS13510 at 805.228 kb on - strand at 805.309 kb on - strand at 805.441 kb on + strand at 805.603 kb on - strand, within BBR_RS13515 at 805.613 kb on + strand, within BBR_RS13515 at 805.667 kb on + strand, within BBR_RS13515 at 805.700 kb on - strand, within BBR_RS13515 at 805.702 kb on + strand, within BBR_RS13515 at 805.716 kb on + strand, within BBR_RS13515 at 805.716 kb on + strand, within BBR_RS13515 at 805.770 kb on + strand, within BBR_RS13515 at 805.775 kb on + strand, within BBR_RS13515 at 805.778 kb on - strand, within BBR_RS13515 at 805.791 kb on + strand, within BBR_RS13515 at 805.804 kb on - strand, within BBR_RS13515 at 805.824 kb on - strand, within BBR_RS13515 at 805.824 kb on - strand, within BBR_RS13515 at 805.824 kb on - strand, within BBR_RS13515 at 805.864 kb on + strand, within BBR_RS13515 at 805.872 kb on - strand, within BBR_RS13515 at 805.931 kb on - strand, within BBR_RS13515 at 805.956 kb on - strand, within BBR_RS13515 at 805.969 kb on + strand, within BBR_RS13515 at 805.969 kb on + strand, within BBR_RS13515 at 806.016 kb on + strand, within BBR_RS13515 at 806.027 kb on - strand, within BBR_RS13515 at 806.027 kb on - strand, within BBR_RS13515 at 806.078 kb on + strand, within BBR_RS13515 at 806.092 kb on - strand, within BBR_RS13515 at 806.120 kb on - strand, within BBR_RS13515 at 806.177 kb on + strand, within BBR_RS13515 at 806.182 kb on + strand, within BBR_RS13515 at 806.187 kb on + strand, within BBR_RS13515 at 806.242 kb on - strand, within BBR_RS13515 at 806.253 kb on + strand, within BBR_RS13515 at 806.309 kb on + strand, within BBR_RS13515 at 806.324 kb on + strand, within BBR_RS13515 at 806.336 kb on - strand, within BBR_RS13515 at 806.336 kb on - strand, within BBR_RS13515 at 806.336 kb on - strand, within BBR_RS13515 at 806.336 kb on - strand, within BBR_RS13515 at 806.336 kb on - strand, within BBR_RS13515 at 806.358 kb on + strand at 806.358 kb on + strand at 806.387 kb on - strand at 806.403 kb on - strand at 806.449 kb on + strand at 806.449 kb on + strand at 806.449 kb on - strand at 806.465 kb on - strand at 806.465 kb on - strand at 806.495 kb on - strand at 806.497 kb on + strand at 806.530 kb on + strand at 806.538 kb on - strand at 806.539 kb on - strand at 806.564 kb on + strand at 806.606 kb on + strand, within BBR_RS13520 at 806.748 kb on + strand, within BBR_RS13520 at 806.767 kb on - strand, within BBR_RS13520 at 806.767 kb on - strand, within BBR_RS13520 at 806.797 kb on + strand, within BBR_RS13520 at 806.821 kb on + strand, within BBR_RS13520 at 806.858 kb on + strand, within BBR_RS13520 at 806.866 kb on + strand, within BBR_RS13520 at 806.874 kb on - strand, within BBR_RS13520 at 806.874 kb on - strand, within BBR_RS13520 at 806.875 kb on + strand, within BBR_RS13520 at 806.876 kb on - strand, within BBR_RS13520 at 806.876 kb on - strand, within BBR_RS13520 at 806.878 kb on - strand, within BBR_RS13520 at 806.892 kb on + strand, within BBR_RS13520 at 806.902 kb on - strand, within BBR_RS13520 at 807.004 kb on - strand at 807.006 kb on - strand at 807.032 kb on - strand at 807.045 kb on - strand at 807.114 kb on + strand at 807.164 kb on - strand, within BBR_RS13525 at 807.164 kb on - strand, within BBR_RS13525 at 807.169 kb on + strand, within BBR_RS13525 at 807.186 kb on - strand, within BBR_RS13525 at 807.189 kb on + strand, within BBR_RS13525 at 807.201 kb on + strand, within BBR_RS13525 at 807.234 kb on + strand, within BBR_RS13525 at 807.253 kb on - strand, within BBR_RS13525 at 807.269 kb on + strand, within BBR_RS13525 at 807.409 kb on + strand, within BBR_RS13525
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Vitagos-prebiotic 0.5 %w/v remove 804,439 - -0.7 804,446 + -0.2 804,446 + +0.1 804,446 + -0.8 804,446 + -0.5 804,446 + -0.1 804,446 + -0.0 804,446 + +0.1 804,454 - -1.0 804,454 - +0.5 804,459 + -0.2 804,467 - -0.6 804,488 - -1.1 804,499 + -2.4 804,506 + -0.6 804,509 - -0.5 804,516 + -0.9 804,516 + -1.7 804,596 + BBR_RS13510 0.16 -0.7 804,599 - BBR_RS13510 0.16 -0.8 804,630 + BBR_RS13510 0.20 -0.6 804,653 - BBR_RS13510 0.23 -0.5 804,783 - BBR_RS13510 0.38 -0.0 804,876 + BBR_RS13510 0.50 -0.4 804,876 + BBR_RS13510 0.50 -0.9 804,884 - BBR_RS13510 0.51 -1.0 804,889 + BBR_RS13510 0.51 +1.1 804,935 + BBR_RS13510 0.57 -1.6 804,935 + BBR_RS13510 0.57 -1.2 804,935 + BBR_RS13510 0.57 -2.5 804,943 - BBR_RS13510 0.58 -2.6 804,943 - BBR_RS13510 0.58 -0.8 804,943 - BBR_RS13510 0.58 +0.3 804,954 - BBR_RS13510 0.59 -1.4 804,956 + BBR_RS13510 0.60 +0.6 804,991 - BBR_RS13510 0.64 +0.2 805,007 + BBR_RS13510 0.66 -0.8 805,007 + BBR_RS13510 0.66 -1.1 805,028 - BBR_RS13510 0.69 -0.5 805,056 - BBR_RS13510 0.72 -1.5 805,061 - BBR_RS13510 0.73 +0.9 805,078 - BBR_RS13510 0.75 +0.1 805,080 + BBR_RS13510 0.75 +0.2 805,126 - BBR_RS13510 0.81 -1.1 805,126 - BBR_RS13510 0.81 +0.3 805,152 + BBR_RS13510 0.84 -0.7 805,152 + BBR_RS13510 0.84 +0.1 805,154 + BBR_RS13510 0.84 -1.4 805,162 - BBR_RS13510 0.85 -2.4 805,228 - -1.3 805,309 - +0.3 805,441 + -1.9 805,603 - BBR_RS13515 0.19 +0.1 805,613 + BBR_RS13515 0.20 -2.2 805,667 + BBR_RS13515 0.25 -0.1 805,700 - BBR_RS13515 0.28 -1.2 805,702 + BBR_RS13515 0.28 -1.3 805,716 + BBR_RS13515 0.30 -3.3 805,716 + BBR_RS13515 0.30 -0.4 805,770 + BBR_RS13515 0.35 -1.6 805,775 + BBR_RS13515 0.35 -0.7 805,778 - BBR_RS13515 0.36 -2.2 805,791 + BBR_RS13515 0.37 -2.4 805,804 - BBR_RS13515 0.38 -1.4 805,824 - BBR_RS13515 0.40 -1.1 805,824 - BBR_RS13515 0.40 +0.1 805,824 - BBR_RS13515 0.40 +0.1 805,864 + BBR_RS13515 0.44 -1.9 805,872 - BBR_RS13515 0.44 -0.2 805,931 - BBR_RS13515 0.50 -1.2 805,956 - BBR_RS13515 0.52 -0.7 805,969 + BBR_RS13515 0.54 +0.4 805,969 + BBR_RS13515 0.54 -3.0 806,016 + BBR_RS13515 0.58 -2.7 806,027 - BBR_RS13515 0.59 -0.7 806,027 - BBR_RS13515 0.59 -0.1 806,078 + BBR_RS13515 0.64 -1.5 806,092 - BBR_RS13515 0.65 -0.1 806,120 - BBR_RS13515 0.68 +1.5 806,177 + BBR_RS13515 0.73 -0.7 806,182 + BBR_RS13515 0.74 -0.9 806,187 + BBR_RS13515 0.74 -0.3 806,242 - BBR_RS13515 0.79 -1.9 806,253 + BBR_RS13515 0.80 -1.7 806,309 + BBR_RS13515 0.85 -0.7 806,324 + BBR_RS13515 0.87 -0.9 806,336 - BBR_RS13515 0.88 -0.9 806,336 - BBR_RS13515 0.88 +0.1 806,336 - BBR_RS13515 0.88 +0.1 806,336 - BBR_RS13515 0.88 +0.1 806,336 - BBR_RS13515 0.88 -0.5 806,358 + -1.2 806,358 + -1.5 806,387 - -2.1 806,403 - -0.3 806,449 + -1.3 806,449 + -1.5 806,449 - -0.2 806,465 - +0.7 806,465 - -0.1 806,495 - +0.9 806,497 + +0.4 806,530 + -0.4 806,538 - -0.1 806,539 - -0.8 806,564 + +0.8 806,606 + BBR_RS13520 0.12 +0.6 806,748 + BBR_RS13520 0.41 +0.4 806,767 - BBR_RS13520 0.45 -2.7 806,767 - BBR_RS13520 0.45 +0.3 806,797 + BBR_RS13520 0.51 +0.6 806,821 + BBR_RS13520 0.56 +0.5 806,858 + BBR_RS13520 0.64 +0.1 806,866 + BBR_RS13520 0.65 +0.3 806,874 - BBR_RS13520 0.67 -0.7 806,874 - BBR_RS13520 0.67 +0.9 806,875 + BBR_RS13520 0.67 -0.4 806,876 - BBR_RS13520 0.67 -0.5 806,876 - BBR_RS13520 0.67 -0.2 806,878 - BBR_RS13520 0.68 +0.1 806,892 + BBR_RS13520 0.70 -0.2 806,902 - BBR_RS13520 0.73 +0.9 807,004 - -0.3 807,006 - +0.9 807,032 - -0.2 807,045 - -1.3 807,114 + +0.5 807,164 - BBR_RS13525 0.13 +0.3 807,164 - BBR_RS13525 0.13 +0.5 807,169 + BBR_RS13525 0.14 +0.5 807,186 - BBR_RS13525 0.19 -0.8 807,189 + BBR_RS13525 0.20 +0.6 807,201 + BBR_RS13525 0.23 -0.8 807,234 + BBR_RS13525 0.32 -0.9 807,253 - BBR_RS13525 0.37 +0.3 807,269 + BBR_RS13525 0.42 -0.9 807,409 + BBR_RS13525 0.80 +0.4
Or see this region's nucleotide sequence