Experiment: MoYLS4 with peroxynitrite 200uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hysB and hysA are separated by 33 nucleotides hysA and DVU1919 are separated by 268 nucleotides
DVU1917: hysB - periplasmic [NiFeSe] hydrogenase, small subunit (TIGR), at 1,991,067 to 1,992,020
hysB
DVU1918: hysA - periplasmic [NiFeSe] hydrogenase, large subunit, selenocysteine-containing (TIGR), at 1,992,054 to 1,993,586
hysA
DVU1919: DVU1919 - hydrogenase expression/formation protein, putative (TIGR), at 1,993,855 to 1,994,325
DVU1919
Position (kb)
1992
1993
1994 Strain fitness (log2 ratio)
-2
-1
0
1 at 1991.079 kb on - strand at 1991.126 kb on - strand at 1991.251 kb on + strand, within hysB at 1991.251 kb on - strand, within hysB at 1991.402 kb on - strand, within hysB at 1991.735 kb on + strand, within hysB at 1991.779 kb on + strand, within hysB at 1991.834 kb on - strand, within hysB at 1991.834 kb on - strand, within hysB at 1991.929 kb on - strand at 1991.984 kb on - strand at 1992.151 kb on - strand at 1992.170 kb on + strand at 1992.178 kb on - strand at 1992.299 kb on - strand, within hysA at 1992.337 kb on + strand, within hysA at 1992.432 kb on + strand, within hysA at 1992.471 kb on + strand, within hysA at 1992.487 kb on + strand, within hysA at 1992.489 kb on - strand, within hysA at 1992.546 kb on - strand, within hysA at 1992.596 kb on + strand, within hysA at 1992.680 kb on + strand, within hysA at 1992.728 kb on - strand, within hysA at 1992.758 kb on + strand, within hysA at 1992.774 kb on + strand, within hysA at 1992.776 kb on + strand, within hysA at 1992.784 kb on - strand, within hysA at 1992.784 kb on - strand, within hysA at 1992.860 kb on + strand, within hysA at 1992.868 kb on - strand, within hysA at 1992.869 kb on - strand, within hysA at 1992.995 kb on - strand, within hysA at 1993.050 kb on + strand, within hysA at 1993.073 kb on + strand, within hysA at 1993.095 kb on - strand, within hysA at 1993.201 kb on + strand, within hysA at 1993.275 kb on + strand, within hysA at 1993.275 kb on + strand, within hysA at 1993.301 kb on - strand, within hysA at 1993.334 kb on - strand, within hysA at 1993.343 kb on + strand, within hysA at 1993.422 kb on - strand, within hysA at 1993.518 kb on + strand at 1993.518 kb on + strand at 1993.523 kb on + strand at 1993.531 kb on - strand at 1993.711 kb on + strand at 1993.729 kb on + strand at 1993.768 kb on + strand at 1993.814 kb on + strand at 1993.959 kb on + strand, within DVU1919 at 1994.069 kb on - strand, within DVU1919 at 1994.184 kb on - strand, within DVU1919
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with peroxynitrite 200uM remove 1,991,079 - +0.7 1,991,126 - -0.3 1,991,251 + hysB DVU1917 0.19 -1.3 1,991,251 - hysB DVU1917 0.19 -0.9 1,991,402 - hysB DVU1917 0.35 -1.5 1,991,735 + hysB DVU1917 0.70 +0.4 1,991,779 + hysB DVU1917 0.75 -0.6 1,991,834 - hysB DVU1917 0.80 -0.9 1,991,834 - hysB DVU1917 0.80 -2.0 1,991,929 - -1.0 1,991,984 - -0.9 1,992,151 - -1.3 1,992,170 + +0.7 1,992,178 - -1.6 1,992,299 - hysA DVU1918 0.16 -0.3 1,992,337 + hysA DVU1918 0.18 +1.7 1,992,432 + hysA DVU1918 0.25 -0.8 1,992,471 + hysA DVU1918 0.27 -1.1 1,992,487 + hysA DVU1918 0.28 -1.8 1,992,489 - hysA DVU1918 0.28 -1.2 1,992,546 - hysA DVU1918 0.32 -0.9 1,992,596 + hysA DVU1918 0.35 -1.0 1,992,680 + hysA DVU1918 0.41 -1.1 1,992,728 - hysA DVU1918 0.44 +0.4 1,992,758 + hysA DVU1918 0.46 -1.3 1,992,774 + hysA DVU1918 0.47 -0.9 1,992,776 + hysA DVU1918 0.47 -2.0 1,992,784 - hysA DVU1918 0.48 -0.3 1,992,784 - hysA DVU1918 0.48 -0.9 1,992,860 + hysA DVU1918 0.53 +0.2 1,992,868 - hysA DVU1918 0.53 +0.0 1,992,869 - hysA DVU1918 0.53 -0.9 1,992,995 - hysA DVU1918 0.61 -1.2 1,993,050 + hysA DVU1918 0.65 -0.8 1,993,073 + hysA DVU1918 0.66 -0.7 1,993,095 - hysA DVU1918 0.68 +0.7 1,993,201 + hysA DVU1918 0.75 -1.1 1,993,275 + hysA DVU1918 0.80 -0.3 1,993,275 + hysA DVU1918 0.80 -0.5 1,993,301 - hysA DVU1918 0.81 -0.8 1,993,334 - hysA DVU1918 0.83 -1.7 1,993,343 + hysA DVU1918 0.84 -0.8 1,993,422 - hysA DVU1918 0.89 -0.1 1,993,518 + -2.3 1,993,518 + -1.5 1,993,523 + +0.7 1,993,531 - -1.1 1,993,711 + +1.7 1,993,729 + +0.5 1,993,768 + +0.4 1,993,814 + -0.6 1,993,959 + DVU1919 0.22 -1.1 1,994,069 - DVU1919 0.45 -1.8 1,994,184 - DVU1919 0.70 -1.2
Or see this region's nucleotide sequence