Strain Fitness in Desulfovibrio vulgaris Hildenborough JW710 around DVU1918

Experiment: MoYLS4 with peroxynitrite 200uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthysB and hysA are separated by 33 nucleotideshysA and DVU1919 are separated by 268 nucleotides DVU1917: hysB - periplasmic [NiFeSe] hydrogenase, small subunit (TIGR), at 1,991,067 to 1,992,020 hysB DVU1918: hysA - periplasmic [NiFeSe] hydrogenase, large subunit, selenocysteine-containing (TIGR), at 1,992,054 to 1,993,586 hysA DVU1919: DVU1919 - hydrogenase expression/formation protein, putative (TIGR), at 1,993,855 to 1,994,325 DVU1919 Position (kb) 1992 1993 1994Strain fitness (log2 ratio) -2 -1 0 1at 1991.079 kb on - strandat 1991.126 kb on - strandat 1991.251 kb on + strand, within hysBat 1991.251 kb on - strand, within hysBat 1991.402 kb on - strand, within hysBat 1991.735 kb on + strand, within hysBat 1991.779 kb on + strand, within hysBat 1991.834 kb on - strand, within hysBat 1991.834 kb on - strand, within hysBat 1991.929 kb on - strandat 1991.984 kb on - strandat 1992.151 kb on - strandat 1992.170 kb on + strandat 1992.178 kb on - strandat 1992.299 kb on - strand, within hysAat 1992.337 kb on + strand, within hysAat 1992.432 kb on + strand, within hysAat 1992.471 kb on + strand, within hysAat 1992.487 kb on + strand, within hysAat 1992.489 kb on - strand, within hysAat 1992.546 kb on - strand, within hysAat 1992.596 kb on + strand, within hysAat 1992.680 kb on + strand, within hysAat 1992.728 kb on - strand, within hysAat 1992.758 kb on + strand, within hysAat 1992.774 kb on + strand, within hysAat 1992.776 kb on + strand, within hysAat 1992.784 kb on - strand, within hysAat 1992.784 kb on - strand, within hysAat 1992.860 kb on + strand, within hysAat 1992.868 kb on - strand, within hysAat 1992.869 kb on - strand, within hysAat 1992.995 kb on - strand, within hysAat 1993.050 kb on + strand, within hysAat 1993.073 kb on + strand, within hysAat 1993.095 kb on - strand, within hysAat 1993.201 kb on + strand, within hysAat 1993.275 kb on + strand, within hysAat 1993.275 kb on + strand, within hysAat 1993.301 kb on - strand, within hysAat 1993.334 kb on - strand, within hysAat 1993.343 kb on + strand, within hysAat 1993.422 kb on - strand, within hysAat 1993.518 kb on + strandat 1993.518 kb on + strandat 1993.523 kb on + strandat 1993.531 kb on - strandat 1993.711 kb on + strandat 1993.729 kb on + strandat 1993.768 kb on + strandat 1993.814 kb on + strandat 1993.959 kb on + strand, within DVU1919at 1994.069 kb on - strand, within DVU1919at 1994.184 kb on - strand, within DVU1919

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Per-strain Table

Position Strand Gene LocusTag Fraction MoYLS4 with peroxynitrite 200uM
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1,991,079 - +0.7
1,991,126 - -0.3
1,991,251 + hysB DVU1917 0.19 -1.3
1,991,251 - hysB DVU1917 0.19 -0.9
1,991,402 - hysB DVU1917 0.35 -1.5
1,991,735 + hysB DVU1917 0.70 +0.4
1,991,779 + hysB DVU1917 0.75 -0.6
1,991,834 - hysB DVU1917 0.80 -0.9
1,991,834 - hysB DVU1917 0.80 -2.0
1,991,929 - -1.0
1,991,984 - -0.9
1,992,151 - -1.3
1,992,170 + +0.7
1,992,178 - -1.6
1,992,299 - hysA DVU1918 0.16 -0.3
1,992,337 + hysA DVU1918 0.18 +1.7
1,992,432 + hysA DVU1918 0.25 -0.8
1,992,471 + hysA DVU1918 0.27 -1.1
1,992,487 + hysA DVU1918 0.28 -1.8
1,992,489 - hysA DVU1918 0.28 -1.2
1,992,546 - hysA DVU1918 0.32 -0.9
1,992,596 + hysA DVU1918 0.35 -1.0
1,992,680 + hysA DVU1918 0.41 -1.1
1,992,728 - hysA DVU1918 0.44 +0.4
1,992,758 + hysA DVU1918 0.46 -1.3
1,992,774 + hysA DVU1918 0.47 -0.9
1,992,776 + hysA DVU1918 0.47 -2.0
1,992,784 - hysA DVU1918 0.48 -0.3
1,992,784 - hysA DVU1918 0.48 -0.9
1,992,860 + hysA DVU1918 0.53 +0.2
1,992,868 - hysA DVU1918 0.53 +0.0
1,992,869 - hysA DVU1918 0.53 -0.9
1,992,995 - hysA DVU1918 0.61 -1.2
1,993,050 + hysA DVU1918 0.65 -0.8
1,993,073 + hysA DVU1918 0.66 -0.7
1,993,095 - hysA DVU1918 0.68 +0.7
1,993,201 + hysA DVU1918 0.75 -1.1
1,993,275 + hysA DVU1918 0.80 -0.3
1,993,275 + hysA DVU1918 0.80 -0.5
1,993,301 - hysA DVU1918 0.81 -0.8
1,993,334 - hysA DVU1918 0.83 -1.7
1,993,343 + hysA DVU1918 0.84 -0.8
1,993,422 - hysA DVU1918 0.89 -0.1
1,993,518 + -2.3
1,993,518 + -1.5
1,993,523 + +0.7
1,993,531 - -1.1
1,993,711 + +1.7
1,993,729 + +0.5
1,993,768 + +0.4
1,993,814 + -0.6
1,993,959 + DVU1919 0.22 -1.1
1,994,069 - DVU1919 0.45 -1.8
1,994,184 - DVU1919 0.70 -1.2

Or see this region's nucleotide sequence