Experiment: m.b. Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3040 and Dshi_3041 are separated by 118 nucleotides Dshi_3041 and Dshi_3042 are separated by 3 nucleotides Dshi_3042 and Dshi_3043 overlap by 1 nucleotides Dshi_3043 and Dshi_3044 are separated by 122 nucleotides
Dshi_3040: Dshi_3040 - Dihydrodipicolinate reductase (RefSeq), at 3,209,163 to 3,209,972
_3040
Dshi_3041: Dshi_3041 - ribosome-binding factor A (RefSeq), at 3,210,091 to 3,210,498
_3041
Dshi_3042: Dshi_3042 - tRNA pseudouridine synthase B (RefSeq), at 3,210,502 to 3,211,416
_3042
Dshi_3043: Dshi_3043 - protein of unknown function DUF1294 (RefSeq), at 3,211,416 to 3,211,694
_3043
Dshi_3044: Dshi_3044 - Feruloyl esterase (RefSeq), at 3,211,817 to 3,213,247
_3044
Position (kb)
3210
3211
3212 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3209.990 kb on - strand at 3210.090 kb on - strand at 3210.251 kb on + strand, within Dshi_3041 at 3210.275 kb on + strand, within Dshi_3041 at 3210.375 kb on - strand, within Dshi_3041 at 3210.492 kb on + strand at 3210.499 kb on + strand at 3210.537 kb on - strand at 3210.580 kb on + strand at 3210.599 kb on - strand, within Dshi_3042 at 3210.766 kb on - strand, within Dshi_3042 at 3210.873 kb on + strand, within Dshi_3042 at 3211.017 kb on + strand, within Dshi_3042 at 3211.112 kb on + strand, within Dshi_3042 at 3211.182 kb on + strand, within Dshi_3042 at 3211.343 kb on - strand at 3211.385 kb on - strand at 3211.672 kb on - strand at 3211.762 kb on - strand at 3211.766 kb on - strand at 3211.800 kb on + strand at 3211.839 kb on + strand at 3212.064 kb on - strand, within Dshi_3044 at 3212.122 kb on + strand, within Dshi_3044 at 3212.125 kb on - strand, within Dshi_3044 at 3212.295 kb on - strand, within Dshi_3044
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Dimethyl Sulfoxide 7.5 vol% remove 3,209,990 - -1.0 3,210,090 - -0.1 3,210,251 + Dshi_3041 0.39 +2.6 3,210,275 + Dshi_3041 0.45 +1.3 3,210,375 - Dshi_3041 0.70 +0.9 3,210,492 + +0.8 3,210,499 + +0.3 3,210,537 - +1.1 3,210,580 + +0.5 3,210,599 - Dshi_3042 0.11 +0.4 3,210,766 - Dshi_3042 0.29 -0.3 3,210,873 + Dshi_3042 0.41 +1.3 3,211,017 + Dshi_3042 0.56 +1.2 3,211,112 + Dshi_3042 0.67 -0.0 3,211,182 + Dshi_3042 0.74 -0.2 3,211,343 - +0.1 3,211,385 - -0.9 3,211,672 - -0.0 3,211,762 - -1.1 3,211,766 - -0.1 3,211,800 + +0.8 3,211,839 + +0.2 3,212,064 - Dshi_3044 0.17 +0.5 3,212,122 + Dshi_3044 0.21 -0.1 3,212,125 - Dshi_3044 0.22 -2.0 3,212,295 - Dshi_3044 0.33 -0.2
Or see this region's nucleotide sequence