Experiment: MoYLS4 with peroxynitrite 200uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mutY and DVU0283 are separated by 334 nucleotides DVU0283 and ppiB-1 are separated by 261 nucleotides ppiB-1 and hisH are separated by 187 nucleotides
DVU0282: mutY - A/G-specific adenine glycosylase (TIGR), at 327,314 to 328,435
mutY
DVU0283: DVU0283 - AhpF family protein/thioredoxin reductase (TIGR), at 328,770 to 330,509
DVU0283
DVU0284: ppiB-1 - peptidyl-prolyl cis-trans isomerase B (TIGR), at 330,771 to 331,277
ppiB-1
DVU0285: hisH - IGP synthase, amidotransferase subunit (EC 4.3.2.10) <small>(from data)</small>, at 331,465 to 332,106
hisH
Position (kb) 328
329
330
331 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 327.877 kb on - strand, within mutY at 327.908 kb on - strand, within mutY at 327.960 kb on - strand, within mutY at 327.976 kb on + strand, within mutY at 327.978 kb on + strand, within mutY at 328.025 kb on + strand, within mutY at 328.071 kb on - strand, within mutY at 328.078 kb on + strand, within mutY at 328.078 kb on + strand, within mutY at 328.097 kb on - strand, within mutY at 328.099 kb on + strand, within mutY at 328.107 kb on - strand, within mutY at 328.131 kb on - strand, within mutY at 328.167 kb on - strand, within mutY at 328.199 kb on - strand, within mutY at 328.199 kb on - strand, within mutY at 328.199 kb on - strand, within mutY at 328.254 kb on - strand, within mutY at 328.280 kb on + strand, within mutY at 328.283 kb on - strand, within mutY at 328.322 kb on + strand, within mutY at 328.323 kb on - strand at 328.340 kb on - strand at 328.340 kb on - strand at 328.396 kb on - strand at 328.427 kb on + strand at 328.427 kb on + strand at 328.443 kb on + strand at 328.448 kb on + strand at 328.448 kb on + strand at 328.456 kb on - strand at 328.480 kb on + strand at 328.490 kb on - strand at 328.625 kb on + strand at 328.690 kb on - strand at 328.695 kb on - strand at 328.756 kb on + strand at 328.770 kb on - strand at 328.777 kb on + strand at 328.915 kb on - strand at 328.945 kb on - strand, within DVU0283 at 328.971 kb on - strand, within DVU0283 at 328.971 kb on - strand, within DVU0283 at 329.002 kb on - strand, within DVU0283 at 329.022 kb on + strand, within DVU0283 at 329.061 kb on + strand, within DVU0283 at 329.081 kb on + strand, within DVU0283 at 329.089 kb on - strand, within DVU0283 at 329.168 kb on - strand, within DVU0283 at 329.179 kb on - strand, within DVU0283 at 329.188 kb on - strand, within DVU0283 at 329.190 kb on + strand, within DVU0283 at 329.223 kb on + strand, within DVU0283 at 329.288 kb on + strand, within DVU0283 at 329.388 kb on + strand, within DVU0283 at 329.424 kb on + strand, within DVU0283 at 329.429 kb on - strand, within DVU0283 at 329.522 kb on + strand, within DVU0283 at 329.559 kb on - strand, within DVU0283 at 329.601 kb on - strand, within DVU0283 at 329.604 kb on + strand, within DVU0283 at 329.638 kb on + strand, within DVU0283 at 329.638 kb on + strand, within DVU0283 at 329.696 kb on + strand, within DVU0283 at 329.711 kb on - strand, within DVU0283 at 329.727 kb on + strand, within DVU0283 at 329.752 kb on + strand, within DVU0283 at 329.760 kb on - strand, within DVU0283 at 329.796 kb on + strand, within DVU0283 at 329.870 kb on + strand, within DVU0283 at 329.895 kb on + strand, within DVU0283 at 329.993 kb on + strand, within DVU0283 at 329.993 kb on + strand, within DVU0283 at 329.993 kb on + strand, within DVU0283 at 330.001 kb on - strand, within DVU0283 at 330.001 kb on - strand, within DVU0283 at 330.001 kb on - strand, within DVU0283 at 330.020 kb on - strand, within DVU0283 at 330.054 kb on - strand, within DVU0283 at 330.061 kb on + strand, within DVU0283 at 330.086 kb on + strand, within DVU0283 at 330.086 kb on + strand, within DVU0283 at 330.179 kb on + strand, within DVU0283 at 330.179 kb on - strand, within DVU0283 at 330.184 kb on + strand, within DVU0283 at 330.187 kb on - strand, within DVU0283 at 330.289 kb on - strand, within DVU0283 at 330.343 kb on + strand at 330.343 kb on + strand at 330.382 kb on - strand at 330.398 kb on + strand at 330.442 kb on - strand at 330.442 kb on - strand at 330.444 kb on - strand at 330.557 kb on - strand at 330.592 kb on - strand at 330.599 kb on + strand at 330.604 kb on + strand at 330.657 kb on + strand at 330.726 kb on - strand at 330.891 kb on - strand, within ppiB-1 at 330.910 kb on - strand, within ppiB-1 at 330.915 kb on + strand, within ppiB-1 at 330.973 kb on + strand, within ppiB-1 at 330.989 kb on - strand, within ppiB-1 at 331.013 kb on - strand, within ppiB-1 at 331.087 kb on - strand, within ppiB-1 at 331.109 kb on - strand, within ppiB-1 at 331.110 kb on - strand, within ppiB-1 at 331.125 kb on + strand, within ppiB-1 at 331.131 kb on + strand, within ppiB-1 at 331.133 kb on - strand, within ppiB-1 at 331.133 kb on - strand, within ppiB-1 at 331.196 kb on + strand, within ppiB-1 at 331.229 kb on - strand at 331.239 kb on - strand at 331.257 kb on + strand at 331.277 kb on + strand at 331.277 kb on + strand at 331.284 kb on + strand at 331.284 kb on + strand at 331.353 kb on + strand at 331.417 kb on + strand at 331.495 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with peroxynitrite 200uM remove 327,877 - mutY DVU0282 0.50 -0.9 327,908 - mutY DVU0282 0.53 -0.8 327,960 - mutY DVU0282 0.58 -0.7 327,976 + mutY DVU0282 0.59 -0.3 327,978 + mutY DVU0282 0.59 +0.2 328,025 + mutY DVU0282 0.63 -0.5 328,071 - mutY DVU0282 0.67 -0.8 328,078 + mutY DVU0282 0.68 +1.0 328,078 + mutY DVU0282 0.68 -0.3 328,097 - mutY DVU0282 0.70 -0.7 328,099 + mutY DVU0282 0.70 +0.9 328,107 - mutY DVU0282 0.71 -1.0 328,131 - mutY DVU0282 0.73 -1.1 328,167 - mutY DVU0282 0.76 +1.5 328,199 - mutY DVU0282 0.79 +0.5 328,199 - mutY DVU0282 0.79 -0.5 328,199 - mutY DVU0282 0.79 -0.8 328,254 - mutY DVU0282 0.84 -0.7 328,280 + mutY DVU0282 0.86 +1.5 328,283 - mutY DVU0282 0.86 -0.4 328,322 + mutY DVU0282 0.90 -0.2 328,323 - -0.9 328,340 - -0.2 328,340 - +0.1 328,396 - -0.9 328,427 + -0.8 328,427 + +2.8 328,443 + +0.1 328,448 + +2.5 328,448 + -1.4 328,456 - -1.1 328,480 + -0.5 328,490 - -0.1 328,625 + -0.9 328,690 - +1.7 328,695 - +0.8 328,756 + -1.0 328,770 - +0.5 328,777 + -0.9 328,915 - -1.1 328,945 - DVU0283 0.10 -1.2 328,971 - DVU0283 0.12 -0.6 328,971 - DVU0283 0.12 -0.0 329,002 - DVU0283 0.13 +1.5 329,022 + DVU0283 0.14 +0.5 329,061 + DVU0283 0.17 -1.4 329,081 + DVU0283 0.18 -0.2 329,089 - DVU0283 0.18 -0.4 329,168 - DVU0283 0.23 +0.2 329,179 - DVU0283 0.24 -0.7 329,188 - DVU0283 0.24 -0.4 329,190 + DVU0283 0.24 -0.7 329,223 + DVU0283 0.26 +0.2 329,288 + DVU0283 0.30 +1.0 329,388 + DVU0283 0.36 +1.1 329,424 + DVU0283 0.38 -0.1 329,429 - DVU0283 0.38 -0.2 329,522 + DVU0283 0.43 -0.2 329,559 - DVU0283 0.45 +2.0 329,601 - DVU0283 0.48 -0.5 329,604 + DVU0283 0.48 -0.3 329,638 + DVU0283 0.50 -0.5 329,638 + DVU0283 0.50 -1.5 329,696 + DVU0283 0.53 -0.2 329,711 - DVU0283 0.54 +0.2 329,727 + DVU0283 0.55 +0.3 329,752 + DVU0283 0.56 +0.3 329,760 - DVU0283 0.57 +3.8 329,796 + DVU0283 0.59 +0.9 329,870 + DVU0283 0.63 -0.1 329,895 + DVU0283 0.65 +0.0 329,993 + DVU0283 0.70 -0.3 329,993 + DVU0283 0.70 -0.8 329,993 + DVU0283 0.70 -0.4 330,001 - DVU0283 0.71 +2.0 330,001 - DVU0283 0.71 -0.7 330,001 - DVU0283 0.71 -0.4 330,020 - DVU0283 0.72 -0.6 330,054 - DVU0283 0.74 +0.5 330,061 + DVU0283 0.74 +0.6 330,086 + DVU0283 0.76 +0.0 330,086 + DVU0283 0.76 -0.8 330,179 + DVU0283 0.81 -0.1 330,179 - DVU0283 0.81 +1.3 330,184 + DVU0283 0.81 -1.0 330,187 - DVU0283 0.81 +2.2 330,289 - DVU0283 0.87 +1.5 330,343 + -1.1 330,343 + -0.8 330,382 - +3.5 330,398 + -0.3 330,442 - -0.2 330,442 - +2.1 330,444 - +0.5 330,557 - -0.5 330,592 - +0.1 330,599 + +0.5 330,604 + -0.2 330,657 + -0.1 330,726 - +0.1 330,891 - ppiB-1 DVU0284 0.24 -0.5 330,910 - ppiB-1 DVU0284 0.27 -0.3 330,915 + ppiB-1 DVU0284 0.28 -0.1 330,973 + ppiB-1 DVU0284 0.40 +0.2 330,989 - ppiB-1 DVU0284 0.43 +0.5 331,013 - ppiB-1 DVU0284 0.48 -0.4 331,087 - ppiB-1 DVU0284 0.62 -0.8 331,109 - ppiB-1 DVU0284 0.67 -0.4 331,110 - ppiB-1 DVU0284 0.67 -0.3 331,125 + ppiB-1 DVU0284 0.70 -0.6 331,131 + ppiB-1 DVU0284 0.71 -0.5 331,133 - ppiB-1 DVU0284 0.71 -0.5 331,133 - ppiB-1 DVU0284 0.71 +2.1 331,196 + ppiB-1 DVU0284 0.84 -0.5 331,229 - -0.1 331,239 - -1.1 331,257 + -0.1 331,277 + -0.8 331,277 + -0.2 331,284 + -0.4 331,284 + -0.2 331,353 + +0.5 331,417 + +0.2 331,495 + -0.3
Or see this region's nucleotide sequence