Experiment: MoYLS4 with peroxynitrite 200uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0179 and modC overlap by 14 nucleotides modC and modB overlap by 4 nucleotides modB and DVU0182 are separated by 364 nucleotides
DVU0179: DVU0179 - molybdenum-pterin binding domain protein/site-specific recombinase, phage integrase family (TIGR), at 223,208 to 224,605
DVU0179
DVU0180: modC - ATP-binding component of molybdate ABC transporter (Dmitry Rodionov), at 224,592 to 225,353
modC
DVU0181: modB - molybdenum ABC transporter, permease protein (TIGR), at 225,350 to 226,051
modB
DVU0182: DVU0182 - radical SAM domain protein (TIGR), at 226,416 to 227,339
DVU0182
Position (kb) 225
226
227 Strain fitness (log2 ratio) -2
-1
0
1
2 at 224.380 kb on - strand, within DVU0179 at 224.477 kb on + strand at 224.490 kb on - strand at 224.525 kb on - strand at 224.529 kb on - strand at 224.562 kb on - strand at 224.567 kb on - strand at 224.567 kb on - strand at 224.569 kb on + strand at 224.577 kb on - strand at 224.624 kb on + strand at 224.670 kb on + strand, within modC at 224.690 kb on + strand, within modC at 224.730 kb on + strand, within modC at 224.756 kb on - strand, within modC at 224.825 kb on + strand, within modC at 224.856 kb on + strand, within modC at 224.880 kb on + strand, within modC at 224.887 kb on + strand, within modC at 224.895 kb on - strand, within modC at 224.905 kb on - strand, within modC at 225.074 kb on + strand, within modC at 225.081 kb on + strand, within modC at 225.081 kb on + strand, within modC at 225.089 kb on - strand, within modC at 225.159 kb on - strand, within modC at 225.159 kb on - strand, within modC at 225.263 kb on - strand, within modC at 225.320 kb on - strand at 225.376 kb on + strand at 225.382 kb on - strand at 225.402 kb on - strand at 225.415 kb on + strand at 225.437 kb on - strand, within modB at 225.439 kb on - strand, within modB at 225.489 kb on - strand, within modB at 225.495 kb on - strand, within modB at 225.549 kb on + strand, within modB at 225.628 kb on - strand, within modB at 225.643 kb on + strand, within modB at 225.651 kb on - strand, within modB at 225.651 kb on - strand, within modB at 225.651 kb on - strand, within modB at 225.716 kb on - strand, within modB at 225.767 kb on + strand, within modB at 225.802 kb on - strand, within modB at 225.802 kb on - strand, within modB at 225.829 kb on + strand, within modB at 225.837 kb on - strand, within modB at 225.913 kb on - strand, within modB at 225.937 kb on + strand, within modB at 226.018 kb on + strand at 226.047 kb on + strand at 226.082 kb on - strand at 226.209 kb on + strand at 226.278 kb on - strand at 226.287 kb on + strand at 226.295 kb on - strand at 226.300 kb on - strand at 226.310 kb on - strand at 226.361 kb on + strand at 226.379 kb on - strand at 226.391 kb on + strand at 226.417 kb on - strand at 226.417 kb on - strand at 226.463 kb on + strand at 226.518 kb on - strand, within DVU0182 at 226.549 kb on - strand, within DVU0182 at 226.597 kb on + strand, within DVU0182 at 226.597 kb on + strand, within DVU0182 at 226.597 kb on + strand, within DVU0182 at 226.605 kb on - strand, within DVU0182 at 226.605 kb on - strand, within DVU0182 at 226.605 kb on - strand, within DVU0182 at 226.630 kb on + strand, within DVU0182 at 226.660 kb on - strand, within DVU0182 at 226.789 kb on + strand, within DVU0182 at 226.789 kb on + strand, within DVU0182 at 226.790 kb on + strand, within DVU0182 at 226.797 kb on - strand, within DVU0182 at 226.805 kb on + strand, within DVU0182 at 226.810 kb on + strand, within DVU0182 at 226.934 kb on - strand, within DVU0182 at 226.936 kb on + strand, within DVU0182 at 226.939 kb on - strand, within DVU0182 at 226.939 kb on - strand, within DVU0182 at 226.944 kb on - strand, within DVU0182 at 226.958 kb on + strand, within DVU0182 at 227.017 kb on - strand, within DVU0182 at 227.029 kb on - strand, within DVU0182
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with peroxynitrite 200uM remove 224,380 - DVU0179 0.84 -0.1 224,477 + -0.3 224,490 - -0.1 224,525 - -2.4 224,529 - +1.4 224,562 - -0.5 224,567 - +0.0 224,567 - +0.4 224,569 + +0.2 224,577 - -0.2 224,624 + -1.0 224,670 + modC DVU0180 0.10 -0.2 224,690 + modC DVU0180 0.13 -2.1 224,730 + modC DVU0180 0.18 -0.9 224,756 - modC DVU0180 0.22 -1.0 224,825 + modC DVU0180 0.31 -0.4 224,856 + modC DVU0180 0.35 +0.4 224,880 + modC DVU0180 0.38 +0.0 224,887 + modC DVU0180 0.39 -0.2 224,895 - modC DVU0180 0.40 +0.0 224,905 - modC DVU0180 0.41 +0.2 225,074 + modC DVU0180 0.63 +0.3 225,081 + modC DVU0180 0.64 -0.7 225,081 + modC DVU0180 0.64 -0.4 225,089 - modC DVU0180 0.65 +0.2 225,159 - modC DVU0180 0.74 -1.4 225,159 - modC DVU0180 0.74 -0.9 225,263 - modC DVU0180 0.88 -0.5 225,320 - -0.3 225,376 + -0.0 225,382 - -0.7 225,402 - +0.2 225,415 + -0.1 225,437 - modB DVU0181 0.12 -1.0 225,439 - modB DVU0181 0.13 -0.5 225,489 - modB DVU0181 0.20 -0.4 225,495 - modB DVU0181 0.21 +0.1 225,549 + modB DVU0181 0.28 -0.1 225,628 - modB DVU0181 0.40 -0.2 225,643 + modB DVU0181 0.42 -0.8 225,651 - modB DVU0181 0.43 -1.6 225,651 - modB DVU0181 0.43 +0.2 225,651 - modB DVU0181 0.43 -1.1 225,716 - modB DVU0181 0.52 -1.5 225,767 + modB DVU0181 0.59 -0.3 225,802 - modB DVU0181 0.64 -0.2 225,802 - modB DVU0181 0.64 -1.0 225,829 + modB DVU0181 0.68 +0.4 225,837 - modB DVU0181 0.69 +0.5 225,913 - modB DVU0181 0.80 +2.4 225,937 + modB DVU0181 0.84 -0.1 226,018 + +0.6 226,047 + -0.3 226,082 - -0.6 226,209 + -0.7 226,278 - +0.4 226,287 + -0.2 226,295 - +0.4 226,300 - -0.1 226,310 - -1.7 226,361 + -0.6 226,379 - -0.6 226,391 + -0.8 226,417 - -1.0 226,417 - +0.3 226,463 + -0.5 226,518 - DVU0182 0.11 -0.3 226,549 - DVU0182 0.14 -1.2 226,597 + DVU0182 0.20 +0.0 226,597 + DVU0182 0.20 +1.4 226,597 + DVU0182 0.20 +0.3 226,605 - DVU0182 0.20 -0.1 226,605 - DVU0182 0.20 +0.4 226,605 - DVU0182 0.20 -0.3 226,630 + DVU0182 0.23 -0.3 226,660 - DVU0182 0.26 -0.8 226,789 + DVU0182 0.40 -0.1 226,789 + DVU0182 0.40 +0.4 226,790 + DVU0182 0.40 +1.5 226,797 - DVU0182 0.41 -0.6 226,805 + DVU0182 0.42 -0.0 226,810 + DVU0182 0.43 +0.8 226,934 - DVU0182 0.56 -0.2 226,936 + DVU0182 0.56 -0.2 226,939 - DVU0182 0.57 +1.4 226,939 - DVU0182 0.57 -0.4 226,944 - DVU0182 0.57 -0.6 226,958 + DVU0182 0.59 -0.9 227,017 - DVU0182 0.65 -1.1 227,029 - DVU0182 0.66 +0.2
Or see this region's nucleotide sequence