Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS24530

Experiment: Glycine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS24520 and BWI76_RS24525 are separated by 8 nucleotidesBWI76_RS24525 and BWI76_RS24530 are separated by 151 nucleotidesBWI76_RS24530 and BWI76_RS24535 are separated by 46 nucleotides BWI76_RS24520: BWI76_RS24520 - urease accessory protein UreF, at 5,111,823 to 5,112,497 _RS24520 BWI76_RS24525: BWI76_RS24525 - urease accessory protein UreG, at 5,112,506 to 5,113,123 _RS24525 BWI76_RS24530: BWI76_RS24530 - cytosine/purines uracil thiamine allantoin permease, at 5,113,275 to 5,114,882 _RS24530 BWI76_RS24535: BWI76_RS24535 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD, at 5,114,929 to 5,115,942 _RS24535 Position (kb) 5113 5114 5115Strain fitness (log2 ratio) -2 -1 0 1 2at 5112.392 kb on - strand, within BWI76_RS24520at 5112.511 kb on - strandat 5112.582 kb on - strand, within BWI76_RS24525at 5112.586 kb on + strand, within BWI76_RS24525at 5112.594 kb on - strand, within BWI76_RS24525at 5112.687 kb on - strand, within BWI76_RS24525at 5112.857 kb on - strand, within BWI76_RS24525at 5113.124 kb on + strandat 5113.124 kb on + strandat 5113.140 kb on + strandat 5113.140 kb on + strandat 5113.234 kb on - strandat 5113.241 kb on + strandat 5113.313 kb on + strandat 5113.329 kb on - strandat 5113.348 kb on + strandat 5113.356 kb on - strandat 5113.392 kb on - strandat 5113.539 kb on - strand, within BWI76_RS24530at 5113.570 kb on - strand, within BWI76_RS24530at 5113.570 kb on - strand, within BWI76_RS24530at 5113.581 kb on - strand, within BWI76_RS24530at 5113.721 kb on + strand, within BWI76_RS24530at 5113.799 kb on + strand, within BWI76_RS24530at 5113.941 kb on - strand, within BWI76_RS24530at 5113.945 kb on + strand, within BWI76_RS24530at 5113.953 kb on - strand, within BWI76_RS24530at 5114.064 kb on + strand, within BWI76_RS24530at 5114.064 kb on + strand, within BWI76_RS24530at 5114.148 kb on + strand, within BWI76_RS24530at 5114.150 kb on + strand, within BWI76_RS24530at 5114.150 kb on + strand, within BWI76_RS24530at 5114.156 kb on - strandat 5114.174 kb on - strand, within BWI76_RS24530at 5114.229 kb on - strand, within BWI76_RS24530at 5114.372 kb on - strand, within BWI76_RS24530at 5114.381 kb on + strand, within BWI76_RS24530at 5114.389 kb on - strand, within BWI76_RS24530at 5114.428 kb on + strand, within BWI76_RS24530at 5114.565 kb on + strand, within BWI76_RS24530at 5114.710 kb on + strand, within BWI76_RS24530at 5114.718 kb on - strand, within BWI76_RS24530at 5114.757 kb on - strandat 5114.762 kb on - strandat 5114.864 kb on + strandat 5114.864 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Glycine (N)
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5,112,392 - BWI76_RS24520 0.84 +0.0
5,112,511 - -1.9
5,112,582 - BWI76_RS24525 0.12 +0.1
5,112,586 + BWI76_RS24525 0.13 +1.3
5,112,594 - BWI76_RS24525 0.14 -1.4
5,112,687 - BWI76_RS24525 0.29 +1.0
5,112,857 - BWI76_RS24525 0.57 +0.5
5,113,124 + +1.0
5,113,124 + -0.9
5,113,140 + +0.2
5,113,140 + -0.3
5,113,234 - -0.6
5,113,241 + -0.6
5,113,313 + +1.3
5,113,329 - +1.6
5,113,348 + +0.9
5,113,356 - -0.0
5,113,392 - +0.2
5,113,539 - BWI76_RS24530 0.16 -0.4
5,113,570 - BWI76_RS24530 0.18 +0.7
5,113,570 - BWI76_RS24530 0.18 +1.1
5,113,581 - BWI76_RS24530 0.19 +0.4
5,113,721 + BWI76_RS24530 0.28 +1.0
5,113,799 + BWI76_RS24530 0.33 +0.6
5,113,941 - BWI76_RS24530 0.41 +0.1
5,113,945 + BWI76_RS24530 0.42 +0.0
5,113,953 - BWI76_RS24530 0.42 -0.6
5,114,064 + BWI76_RS24530 0.49 +0.3
5,114,064 + BWI76_RS24530 0.49 +0.2
5,114,148 + BWI76_RS24530 0.54 +0.1
5,114,150 + BWI76_RS24530 0.54 -0.1
5,114,150 + BWI76_RS24530 0.54 +0.4
5,114,156 - -0.4
5,114,174 - BWI76_RS24530 0.56 -0.2
5,114,229 - BWI76_RS24530 0.59 -1.1
5,114,372 - BWI76_RS24530 0.68 +0.4
5,114,381 + BWI76_RS24530 0.69 +0.8
5,114,389 - BWI76_RS24530 0.69 +0.9
5,114,428 + BWI76_RS24530 0.72 -1.1
5,114,565 + BWI76_RS24530 0.80 -0.6
5,114,710 + BWI76_RS24530 0.89 +0.0
5,114,718 - BWI76_RS24530 0.90 +2.4
5,114,757 - -0.3
5,114,762 - -0.1
5,114,864 + -1.2
5,114,864 + -2.6

Or see this region's nucleotide sequence