Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19230

Experiment: Glycine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19220 and BWI76_RS19225 are separated by 14 nucleotidesBWI76_RS19225 and BWI76_RS19230 are separated by 0 nucleotidesBWI76_RS19230 and BWI76_RS19235 overlap by 4 nucleotidesBWI76_RS19235 and BWI76_RS19240 are separated by 64 nucleotides BWI76_RS19220: BWI76_RS19220 - hypothetical protein, at 4,000,083 to 4,001,201 _RS19220 BWI76_RS19225: BWI76_RS19225 - AsnC family transcriptional regulator, at 4,001,216 to 4,001,671 _RS19225 BWI76_RS19230: BWI76_RS19230 - polar amino acid ABC transporter inner membrane subunit, at 4,001,672 to 4,002,397 _RS19230 BWI76_RS19235: BWI76_RS19235 - polar amino acid ABC transporter inner membrane subunit, at 4,002,394 to 4,003,086 _RS19235 BWI76_RS19240: BWI76_RS19240 - ABC transporter substrate-binding protein, at 4,003,151 to 4,003,924 _RS19240 Position (kb) 4001 4002 4003Strain fitness (log2 ratio) -2 -1 0 1at 4000.760 kb on - strand, within BWI76_RS19220at 4000.969 kb on + strand, within BWI76_RS19220at 4001.044 kb on + strand, within BWI76_RS19220at 4001.319 kb on - strand, within BWI76_RS19225at 4001.345 kb on - strand, within BWI76_RS19225at 4001.401 kb on + strand, within BWI76_RS19225at 4001.570 kb on + strand, within BWI76_RS19225at 4001.596 kb on - strand, within BWI76_RS19225at 4001.845 kb on - strandat 4001.930 kb on - strand, within BWI76_RS19230at 4002.240 kb on + strand, within BWI76_RS19230at 4002.248 kb on - strand, within BWI76_RS19230at 4002.408 kb on - strandat 4002.436 kb on - strandat 4002.533 kb on + strand, within BWI76_RS19235at 4002.556 kb on + strand, within BWI76_RS19235at 4002.588 kb on + strand, within BWI76_RS19235at 4002.643 kb on + strand, within BWI76_RS19235at 4002.836 kb on - strand, within BWI76_RS19235at 4003.014 kb on + strand, within BWI76_RS19235at 4003.060 kb on + strandat 4003.221 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Glycine (N)
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4,000,760 - BWI76_RS19220 0.61 +0.0
4,000,969 + BWI76_RS19220 0.79 -0.2
4,001,044 + BWI76_RS19220 0.86 -0.4
4,001,319 - BWI76_RS19225 0.23 -0.5
4,001,345 - BWI76_RS19225 0.28 -0.0
4,001,401 + BWI76_RS19225 0.41 -1.3
4,001,570 + BWI76_RS19225 0.78 +1.0
4,001,596 - BWI76_RS19225 0.83 +0.5
4,001,845 - +1.1
4,001,930 - BWI76_RS19230 0.36 -0.2
4,002,240 + BWI76_RS19230 0.78 +1.2
4,002,248 - BWI76_RS19230 0.79 +0.3
4,002,408 - -0.5
4,002,436 - -0.3
4,002,533 + BWI76_RS19235 0.20 -0.9
4,002,556 + BWI76_RS19235 0.23 -1.8
4,002,588 + BWI76_RS19235 0.28 -0.8
4,002,643 + BWI76_RS19235 0.36 -1.5
4,002,836 - BWI76_RS19235 0.64 -0.1
4,003,014 + BWI76_RS19235 0.89 -0.3
4,003,060 + +0.3
4,003,221 + -0.8

Or see this region's nucleotide sequence