Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13280 and BWI76_RS13285 are separated by 172 nucleotides BWI76_RS13285 and BWI76_RS13290 overlap by 4 nucleotides BWI76_RS13290 and BWI76_RS13295 are separated by 239 nucleotides
BWI76_RS13280: BWI76_RS13280 - LysR family transcriptional regulator, at 2,768,905 to 2,769,828
_RS13280
BWI76_RS13285: BWI76_RS13285 - protocatechuate 3,4-dioxygenase subunit alpha, at 2,770,001 to 2,770,621
_RS13285
BWI76_RS13290: BWI76_RS13290 - protocatechuate 3,4-dioxygenase subunit beta, at 2,770,618 to 2,771,358
_RS13290
BWI76_RS13295: BWI76_RS13295 - DUF1338 domain-containing protein, at 2,771,598 to 2,772,941
_RS13295
Position (kb)
2770
2771
2772 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2769.700 kb on + strand, within BWI76_RS13280 at 2769.728 kb on - strand, within BWI76_RS13280 at 2769.757 kb on - strand at 2769.783 kb on - strand at 2769.794 kb on + strand at 2770.020 kb on + strand at 2770.256 kb on + strand, within BWI76_RS13285 at 2770.653 kb on + strand at 2770.661 kb on - strand at 2770.888 kb on + strand, within BWI76_RS13290 at 2770.919 kb on - strand, within BWI76_RS13290 at 2771.203 kb on - strand, within BWI76_RS13290 at 2771.475 kb on + strand at 2771.602 kb on - strand at 2771.622 kb on - strand at 2771.689 kb on - strand at 2771.718 kb on - strand at 2771.737 kb on + strand, within BWI76_RS13295 at 2771.759 kb on + strand, within BWI76_RS13295 at 2772.295 kb on + strand, within BWI76_RS13295
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 2,769,700 + BWI76_RS13280 0.86 +1.0 2,769,728 - BWI76_RS13280 0.89 +0.2 2,769,757 - -2.0 2,769,783 - -0.4 2,769,794 + -0.7 2,770,020 + +0.3 2,770,256 + BWI76_RS13285 0.41 +1.1 2,770,653 + -0.7 2,770,661 - +0.6 2,770,888 + BWI76_RS13290 0.36 +1.6 2,770,919 - BWI76_RS13290 0.41 -0.2 2,771,203 - BWI76_RS13290 0.79 -0.2 2,771,475 + +0.2 2,771,602 - -0.8 2,771,622 - +0.8 2,771,689 - -0.1 2,771,718 - +0.6 2,771,737 + BWI76_RS13295 0.10 -0.5 2,771,759 + BWI76_RS13295 0.12 -0.7 2,772,295 + BWI76_RS13295 0.52 -4.2
Or see this region's nucleotide sequence