Experiment: m.b. Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_4173 and Dshi_4174 are separated by 70 nucleotides Dshi_4174 and Dshi_4175 are separated by 63 nucleotides Dshi_4175 and Dshi_4176 are separated by 163 nucleotides Dshi_4176 and Dshi_4177 are separated by 91 nucleotides
Dshi_4173: Dshi_4173 - hypothetical protein (RefSeq), at 22,428 to 23,387
_4173
Dshi_4174: Dshi_4174 - hypothetical protein (RefSeq), at 23,458 to 24,015
_4174
Dshi_4175: Dshi_4175 - hypothetical protein (RefSeq), at 24,079 to 25,098
_4175
Dshi_4176: Dshi_4176 - oxidoreductase FAD/NAD(P)-binding domain protein (RefSeq), at 25,262 to 25,924
_4176
Dshi_4177: Dshi_4177 - Acetamidase/Formamidase (RefSeq), at 26,016 to 27,332
_4177
Position (kb)
24
25
26 Strain fitness (log2 ratio)
-1
0
1
2 at 23.679 kb on + strand, within Dshi_4174 at 23.708 kb on + strand, within Dshi_4174 at 23.848 kb on + strand, within Dshi_4174 at 23.856 kb on - strand, within Dshi_4174 at 24.157 kb on - strand at 24.157 kb on - strand at 24.359 kb on + strand, within Dshi_4175 at 24.443 kb on + strand, within Dshi_4175 at 25.139 kb on - strand at 25.159 kb on + strand at 25.448 kb on - strand at 25.501 kb on + strand, within Dshi_4176 at 25.714 kb on - strand, within Dshi_4176 at 25.745 kb on - strand, within Dshi_4176 at 25.999 kb on + strand at 26.002 kb on - strand at 26.073 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Dimethyl Sulfoxide 5 vol% remove 23,679 + Dshi_4174 0.40 -0.7 23,708 + Dshi_4174 0.45 -1.0 23,848 + Dshi_4174 0.70 -0.8 23,856 - Dshi_4174 0.71 -0.1 24,157 - +1.1 24,157 - +0.1 24,359 + Dshi_4175 0.27 +0.2 24,443 + Dshi_4175 0.36 +1.3 25,139 - -0.2 25,159 + +0.0 25,448 - +1.3 25,501 + Dshi_4176 0.36 -0.3 25,714 - Dshi_4176 0.68 +0.2 25,745 - Dshi_4176 0.73 +0.7 25,999 + -0.3 26,002 - -0.4 26,073 - +1.8
Or see this region's nucleotide sequence