Experiment: m.b. Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1199 and Dshi_1200 are separated by 2 nucleotides Dshi_1200 and Dshi_1201 are separated by 88 nucleotides Dshi_1201 and Dshi_R0025 are separated by 51 nucleotides Dshi_R0025 and Dshi_1202 are separated by 18 nucleotides Dshi_1202 and Dshi_1203 are separated by 72 nucleotides
Dshi_1199: Dshi_1199 - Inositol-phosphate phosphatase (RefSeq), at 1,240,118 to 1,240,912
_1199
Dshi_1200: Dshi_1200 - Rhomboid family protein (RefSeq), at 1,240,915 to 1,241,691
_1200
Dshi_1201: Dshi_1201 - Tetratricopeptide TPR_2 repeat protein (RefSeq), at 1,241,780 to 1,242,331
_1201
Dshi_R0025: Dshi_R0025 - tRNA-Leu (RefSeq), at 1,242,383 to 1,242,468
_R0025
Dshi_1202: Dshi_1202 - heat shock protein DnaJ domain protein (RefSeq), at 1,242,487 to 1,243,116
_1202
Dshi_1203: Dshi_1203 - BolA family protein (RefSeq), at 1,243,189 to 1,243,446
_1203
Position (kb)
1241
1242
1243 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1240.956 kb on + strand at 1241.158 kb on + strand, within Dshi_1200 at 1241.680 kb on + strand at 1241.691 kb on - strand at 1241.700 kb on + strand at 1241.705 kb on + strand at 1242.039 kb on + strand, within Dshi_1201 at 1242.098 kb on - strand, within Dshi_1201 at 1242.214 kb on - strand, within Dshi_1201 at 1242.481 kb on - strand at 1242.935 kb on - strand, within Dshi_1202 at 1242.982 kb on - strand, within Dshi_1202 at 1243.008 kb on + strand, within Dshi_1202 at 1243.008 kb on + strand, within Dshi_1202 at 1243.034 kb on + strand, within Dshi_1202 at 1243.126 kb on + strand at 1243.147 kb on + strand at 1243.155 kb on - strand at 1243.155 kb on - strand at 1243.155 kb on - strand at 1243.166 kb on - strand at 1243.224 kb on - strand, within Dshi_1203 at 1243.230 kb on - strand, within Dshi_1203
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Dimethyl Sulfoxide 5 vol% remove 1,240,956 + -0.1 1,241,158 + Dshi_1200 0.31 +0.1 1,241,680 + -0.4 1,241,691 - -0.1 1,241,700 + +0.4 1,241,705 + -1.0 1,242,039 + Dshi_1201 0.47 -1.0 1,242,098 - Dshi_1201 0.58 +0.3 1,242,214 - Dshi_1201 0.79 +1.3 1,242,481 - +0.2 1,242,935 - Dshi_1202 0.71 -0.6 1,242,982 - Dshi_1202 0.79 -2.7 1,243,008 + Dshi_1202 0.83 -2.3 1,243,008 + Dshi_1202 0.83 -1.3 1,243,034 + Dshi_1202 0.87 -0.6 1,243,126 + +2.6 1,243,147 + +0.2 1,243,155 - -1.5 1,243,155 - -0.5 1,243,155 - +0.4 1,243,166 - -0.6 1,243,224 - Dshi_1203 0.14 -0.9 1,243,230 - Dshi_1203 0.16 -0.0
Or see this region's nucleotide sequence