Experiment: formate McAA thiamine dropout
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01825 and MMP_RS01830 are separated by 105 nucleotides MMP_RS01830 and MMP_RS01835 are separated by 110 nucleotides MMP_RS01835 and MMP_RS01840 are separated by 0 nucleotides
MMP_RS01825: MMP_RS01825 - acetyl-CoA carboxylase biotin carboxylase subunit, at 337,634 to 339,139
_RS01825
MMP_RS01830: MMP_RS01830 - tRNA-Val, at 339,245 to 339,321
_RS01830
MMP_RS01835: MMP_RS01835 - hypothetical protein, at 339,432 to 339,866
_RS01835
MMP_RS01840: MMP_RS01840 - TIGR00289 family protein, at 339,867 to 340,538
_RS01840
Position (kb)
339
340 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 338.812 kb on - strand at 338.887 kb on + strand at 338.949 kb on + strand at 338.959 kb on - strand at 339.094 kb on + strand at 339.122 kb on + strand at 339.152 kb on + strand at 339.155 kb on - strand at 339.160 kb on - strand at 339.160 kb on - strand at 339.166 kb on - strand at 339.166 kb on - strand at 339.206 kb on + strand at 339.215 kb on + strand at 339.224 kb on + strand at 339.234 kb on + strand at 339.240 kb on - strand at 339.257 kb on - strand, within MMP_RS01830 at 339.258 kb on + strand, within MMP_RS01830 at 339.451 kb on + strand at 339.530 kb on - strand, within MMP_RS01835 at 339.535 kb on - strand, within MMP_RS01835 at 339.558 kb on - strand, within MMP_RS01835 at 339.635 kb on - strand, within MMP_RS01835 at 339.635 kb on - strand, within MMP_RS01835 at 339.636 kb on + strand, within MMP_RS01835 at 339.736 kb on + strand, within MMP_RS01835 at 339.737 kb on - strand, within MMP_RS01835 at 339.763 kb on - strand, within MMP_RS01835 at 339.765 kb on - strand, within MMP_RS01835 at 339.812 kb on + strand, within MMP_RS01835 at 339.865 kb on + strand at 339.899 kb on - strand at 339.942 kb on - strand, within MMP_RS01840 at 339.951 kb on - strand, within MMP_RS01840 at 340.266 kb on - strand, within MMP_RS01840 at 340.267 kb on + strand, within MMP_RS01840
Per-strain Table
Position Strand Gene LocusTag Fraction formate McAA thiamine dropout remove 338,812 - -0.2 338,887 + -3.0 338,949 + -3.1 338,959 - -2.7 339,094 + +0.1 339,122 + +0.4 339,152 + +0.5 339,155 - -0.5 339,160 - +0.3 339,160 - +0.1 339,166 - -0.7 339,166 - +0.2 339,206 + +0.9 339,215 + +0.5 339,224 + +0.0 339,234 + -0.1 339,240 - +0.1 339,257 - MMP_RS01830 0.16 -1.1 339,258 + MMP_RS01830 0.17 -0.4 339,451 + -0.1 339,530 - MMP_RS01835 0.23 +0.3 339,535 - MMP_RS01835 0.24 +0.2 339,558 - MMP_RS01835 0.29 +0.8 339,635 - MMP_RS01835 0.47 +0.3 339,635 - MMP_RS01835 0.47 +0.0 339,636 + MMP_RS01835 0.47 +0.4 339,736 + MMP_RS01835 0.70 +0.2 339,737 - MMP_RS01835 0.70 -0.4 339,763 - MMP_RS01835 0.76 -0.0 339,765 - MMP_RS01835 0.77 +0.0 339,812 + MMP_RS01835 0.87 +0.2 339,865 + +0.1 339,899 - -0.3 339,942 - MMP_RS01840 0.11 -0.4 339,951 - MMP_RS01840 0.12 -0.2 340,266 - MMP_RS01840 0.59 -0.5 340,267 + MMP_RS01840 0.60 -0.7
Or see this region's nucleotide sequence