Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04570

Experiment: L-Cysteine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04560 and BWI76_RS04565 are separated by 81 nucleotidesBWI76_RS04565 and BWI76_RS04570 are separated by 12 nucleotidesBWI76_RS04570 and BWI76_RS04575 are separated by 187 nucleotides BWI76_RS04560: BWI76_RS04560 - holo-ACP synthase CitX, at 945,490 to 946,029 _RS04560 BWI76_RS04565: BWI76_RS04565 - bifunctional tRNA pseudouridine(32) synthase/ribosomal large subunit pseudouridine synthase RluA, at 946,111 to 946,770 _RS04565 BWI76_RS04570: BWI76_RS04570 - RNA polymerase-associated protein RapA, at 946,783 to 949,689 _RS04570 BWI76_RS04575: BWI76_RS04575 - DNA polymerase II, at 949,877 to 952,234 _RS04575 Position (kb) 946 947 948 949 950Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 945.809 kb on - strand, within BWI76_RS04560at 946.053 kb on - strandat 946.098 kb on - strandat 946.108 kb on - strandat 946.342 kb on + strand, within BWI76_RS04565at 946.398 kb on + strand, within BWI76_RS04565at 946.407 kb on + strand, within BWI76_RS04565at 946.474 kb on - strand, within BWI76_RS04565at 946.543 kb on + strand, within BWI76_RS04565at 946.554 kb on + strand, within BWI76_RS04565at 947.054 kb on + strandat 947.054 kb on + strandat 947.129 kb on - strand, within BWI76_RS04570at 947.349 kb on + strand, within BWI76_RS04570at 947.595 kb on - strandat 947.824 kb on + strand, within BWI76_RS04570at 948.289 kb on - strand, within BWI76_RS04570at 948.550 kb on - strand, within BWI76_RS04570at 948.641 kb on + strand, within BWI76_RS04570at 948.695 kb on - strand, within BWI76_RS04570at 948.784 kb on - strand, within BWI76_RS04570at 948.965 kb on - strand, within BWI76_RS04570at 948.970 kb on + strand, within BWI76_RS04570at 949.017 kb on + strand, within BWI76_RS04570at 949.025 kb on - strand, within BWI76_RS04570at 949.520 kb on + strandat 949.766 kb on - strandat 949.800 kb on - strandat 949.939 kb on - strandat 949.988 kb on - strandat 950.031 kb on + strandat 950.082 kb on + strandat 950.143 kb on + strand, within BWI76_RS04575at 950.162 kb on + strand, within BWI76_RS04575at 950.170 kb on - strand, within BWI76_RS04575at 950.210 kb on - strand, within BWI76_RS04575at 950.339 kb on - strand, within BWI76_RS04575at 950.463 kb on + strand, within BWI76_RS04575at 950.463 kb on - strand, within BWI76_RS04575

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Cysteine (N)
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945,809 - BWI76_RS04560 0.59 -1.4
946,053 - +0.7
946,098 - +1.1
946,108 - -1.7
946,342 + BWI76_RS04565 0.35 +0.1
946,398 + BWI76_RS04565 0.43 -1.1
946,407 + BWI76_RS04565 0.45 +1.2
946,474 - BWI76_RS04565 0.55 +0.4
946,543 + BWI76_RS04565 0.65 +0.1
946,554 + BWI76_RS04565 0.67 +0.5
947,054 + +1.1
947,054 + -3.2
947,129 - BWI76_RS04570 0.12 -1.7
947,349 + BWI76_RS04570 0.19 -1.0
947,595 - -1.0
947,824 + BWI76_RS04570 0.36 -2.4
948,289 - BWI76_RS04570 0.52 -3.4
948,550 - BWI76_RS04570 0.61 -0.6
948,641 + BWI76_RS04570 0.64 -1.7
948,695 - BWI76_RS04570 0.66 -0.2
948,784 - BWI76_RS04570 0.69 -2.9
948,965 - BWI76_RS04570 0.75 -2.7
948,970 + BWI76_RS04570 0.75 -0.7
949,017 + BWI76_RS04570 0.77 +0.6
949,025 - BWI76_RS04570 0.77 -0.4
949,520 + -0.6
949,766 - -0.2
949,800 - -0.1
949,939 - -0.7
949,988 - +0.7
950,031 + +0.6
950,082 + +0.7
950,143 + BWI76_RS04575 0.11 +1.3
950,162 + BWI76_RS04575 0.12 +0.4
950,170 - BWI76_RS04575 0.12 +0.5
950,210 - BWI76_RS04575 0.14 +1.8
950,339 - BWI76_RS04575 0.20 +0.6
950,463 + BWI76_RS04575 0.25 +0.3
950,463 - BWI76_RS04575 0.25 +0.2

Or see this region's nucleotide sequence