Experiment: m.b. Fusidic 0.0006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3635 and Dshi_3636 are separated by 124 nucleotides Dshi_3636 and Dshi_3637 are separated by 168 nucleotides Dshi_3637 and Dshi_3638 are separated by 43 nucleotides Dshi_3638 and Dshi_3639 overlap by 4 nucleotides Dshi_3639 and Dshi_3640 overlap by 8 nucleotides
Dshi_3635: Dshi_3635 - transcriptional regulator, XRE family (RefSeq), at 37,936 to 38,193
_3635
Dshi_3636: Dshi_3636 - hypothetical protein (RefSeq), at 38,318 to 38,611
_3636
Dshi_3637: Dshi_3637 - hypothetical protein (RefSeq), at 38,780 to 39,133
_3637
Dshi_3638: Dshi_3638 - Lytic transglycosylase catalytic (RefSeq), at 39,177 to 39,797
_3638
Dshi_3639: Dshi_3639 - Conjugal transfer protein TrbC (RefSeq), at 39,794 to 40,087
_3639
Dshi_3640: Dshi_3640 - type IV secretory pathway VirB3 family protein (RefSeq), at 40,080 to 40,358
_3640
Position (kb)
38
39
40 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 37.786 kb on - strand at 37.786 kb on - strand at 37.786 kb on - strand at 37.794 kb on - strand at 37.811 kb on + strand at 37.811 kb on + strand at 37.811 kb on + strand at 37.819 kb on - strand at 37.819 kb on - strand at 37.819 kb on - strand at 37.821 kb on - strand at 37.821 kb on - strand at 37.826 kb on + strand at 37.826 kb on + strand at 37.826 kb on + strand at 37.829 kb on - strand at 37.829 kb on - strand at 37.834 kb on - strand at 37.834 kb on - strand at 37.834 kb on - strand at 37.879 kb on + strand at 37.885 kb on + strand at 37.907 kb on + strand at 37.972 kb on - strand, within Dshi_3635 at 37.972 kb on - strand, within Dshi_3635 at 37.982 kb on + strand, within Dshi_3635 at 37.990 kb on - strand, within Dshi_3635 at 37.992 kb on + strand, within Dshi_3635 at 37.992 kb on + strand, within Dshi_3635 at 37.992 kb on + strand, within Dshi_3635 at 37.992 kb on + strand, within Dshi_3635 at 37.992 kb on + strand, within Dshi_3635 at 37.992 kb on + strand, within Dshi_3635 at 37.992 kb on + strand, within Dshi_3635 at 37.992 kb on + strand, within Dshi_3635 at 37.992 kb on + strand, within Dshi_3635 at 37.992 kb on + strand, within Dshi_3635 at 38.000 kb on - strand, within Dshi_3635 at 38.000 kb on - strand, within Dshi_3635 at 38.000 kb on - strand, within Dshi_3635 at 38.000 kb on - strand, within Dshi_3635 at 38.000 kb on - strand, within Dshi_3635 at 38.000 kb on - strand, within Dshi_3635 at 38.000 kb on - strand, within Dshi_3635 at 38.019 kb on - strand, within Dshi_3635 at 38.092 kb on - strand, within Dshi_3635 at 38.093 kb on - strand, within Dshi_3635 at 38.107 kb on + strand, within Dshi_3635 at 38.107 kb on + strand, within Dshi_3635 at 38.113 kb on + strand, within Dshi_3635 at 38.115 kb on - strand, within Dshi_3635 at 38.115 kb on - strand, within Dshi_3635 at 38.118 kb on + strand, within Dshi_3635 at 38.145 kb on + strand, within Dshi_3635 at 38.148 kb on - strand, within Dshi_3635 at 38.153 kb on - strand, within Dshi_3635 at 38.167 kb on - strand, within Dshi_3635 at 38.277 kb on + strand at 38.286 kb on + strand at 38.288 kb on + strand at 38.294 kb on + strand at 38.294 kb on + strand at 38.296 kb on - strand at 38.298 kb on + strand at 38.302 kb on - strand at 38.302 kb on - strand at 38.345 kb on + strand at 38.358 kb on - strand, within Dshi_3636 at 38.362 kb on + strand, within Dshi_3636 at 38.364 kb on + strand, within Dshi_3636 at 38.371 kb on + strand, within Dshi_3636 at 38.380 kb on + strand, within Dshi_3636 at 38.406 kb on - strand, within Dshi_3636 at 38.406 kb on - strand, within Dshi_3636 at 38.406 kb on - strand, within Dshi_3636 at 38.417 kb on + strand, within Dshi_3636 at 38.430 kb on - strand, within Dshi_3636 at 38.432 kb on + strand, within Dshi_3636 at 38.432 kb on + strand, within Dshi_3636 at 38.432 kb on + strand, within Dshi_3636 at 38.432 kb on + strand, within Dshi_3636 at 38.432 kb on + strand, within Dshi_3636 at 38.440 kb on + strand, within Dshi_3636 at 38.456 kb on + strand, within Dshi_3636 at 38.472 kb on - strand, within Dshi_3636 at 38.474 kb on - strand, within Dshi_3636 at 38.520 kb on + strand, within Dshi_3636 at 38.537 kb on + strand, within Dshi_3636 at 38.541 kb on + strand, within Dshi_3636 at 38.570 kb on + strand, within Dshi_3636 at 38.572 kb on - strand, within Dshi_3636 at 38.588 kb on - strand at 38.590 kb on - strand at 38.590 kb on - strand at 38.613 kb on + strand at 38.615 kb on + strand at 38.623 kb on - strand at 38.636 kb on + strand at 38.644 kb on - strand at 38.651 kb on + strand at 38.651 kb on + strand at 38.741 kb on + strand at 38.741 kb on + strand at 38.741 kb on + strand at 38.783 kb on - strand at 38.785 kb on - strand at 38.913 kb on + strand, within Dshi_3637 at 39.011 kb on + strand, within Dshi_3637 at 39.028 kb on + strand, within Dshi_3637 at 39.042 kb on + strand, within Dshi_3637 at 39.047 kb on + strand, within Dshi_3637 at 39.069 kb on + strand, within Dshi_3637 at 39.077 kb on - strand, within Dshi_3637 at 39.116 kb on - strand at 39.188 kb on - strand at 39.188 kb on - strand at 39.194 kb on - strand at 39.227 kb on + strand at 39.228 kb on - strand at 39.240 kb on - strand, within Dshi_3638 at 39.256 kb on + strand, within Dshi_3638 at 39.274 kb on - strand, within Dshi_3638 at 39.308 kb on + strand, within Dshi_3638 at 39.312 kb on + strand, within Dshi_3638 at 39.320 kb on - strand, within Dshi_3638 at 39.334 kb on + strand, within Dshi_3638 at 39.345 kb on - strand, within Dshi_3638 at 39.360 kb on + strand, within Dshi_3638 at 39.360 kb on + strand, within Dshi_3638 at 39.360 kb on + strand, within Dshi_3638 at 39.368 kb on - strand, within Dshi_3638 at 39.373 kb on - strand, within Dshi_3638 at 39.373 kb on - strand, within Dshi_3638 at 39.380 kb on + strand, within Dshi_3638 at 39.386 kb on - strand, within Dshi_3638 at 39.395 kb on + strand, within Dshi_3638 at 39.402 kb on + strand, within Dshi_3638 at 39.402 kb on + strand, within Dshi_3638 at 39.407 kb on + strand, within Dshi_3638 at 39.410 kb on - strand, within Dshi_3638 at 39.410 kb on - strand, within Dshi_3638 at 39.410 kb on - strand, within Dshi_3638 at 39.426 kb on + strand, within Dshi_3638 at 39.426 kb on + strand, within Dshi_3638 at 39.426 kb on + strand, within Dshi_3638 at 39.430 kb on - strand, within Dshi_3638 at 39.434 kb on - strand, within Dshi_3638 at 39.434 kb on - strand, within Dshi_3638 at 39.434 kb on - strand, within Dshi_3638 at 39.434 kb on - strand, within Dshi_3638 at 39.434 kb on - strand, within Dshi_3638 at 39.434 kb on - strand, within Dshi_3638 at 39.434 kb on - strand, within Dshi_3638 at 39.434 kb on - strand, within Dshi_3638 at 39.434 kb on - strand, within Dshi_3638 at 39.444 kb on - strand, within Dshi_3638 at 39.460 kb on + strand, within Dshi_3638 at 39.473 kb on - strand, within Dshi_3638 at 39.494 kb on - strand, within Dshi_3638 at 39.507 kb on - strand, within Dshi_3638 at 39.521 kb on - strand, within Dshi_3638 at 39.554 kb on + strand, within Dshi_3638 at 39.556 kb on - strand, within Dshi_3638 at 39.556 kb on - strand, within Dshi_3638 at 39.585 kb on - strand, within Dshi_3638 at 39.600 kb on - strand, within Dshi_3638 at 39.602 kb on - strand, within Dshi_3638 at 39.668 kb on - strand, within Dshi_3638 at 39.682 kb on + strand, within Dshi_3638 at 39.697 kb on - strand, within Dshi_3638 at 39.715 kb on + strand, within Dshi_3638 at 39.716 kb on - strand, within Dshi_3638 at 39.735 kb on + strand, within Dshi_3638 at 39.735 kb on + strand, within Dshi_3638 at 39.735 kb on + strand, within Dshi_3638 at 39.742 kb on + strand at 39.743 kb on - strand at 39.743 kb on - strand at 39.743 kb on - strand at 39.743 kb on - strand at 39.743 kb on - strand at 39.748 kb on - strand at 39.779 kb on - strand at 39.811 kb on + strand at 39.848 kb on + strand, within Dshi_3639 at 39.858 kb on + strand, within Dshi_3639 at 39.858 kb on + strand, within Dshi_3639 at 39.858 kb on + strand, within Dshi_3639 at 39.866 kb on - strand, within Dshi_3639 at 39.866 kb on - strand, within Dshi_3639 at 39.866 kb on - strand, within Dshi_3639 at 39.866 kb on - strand, within Dshi_3639 at 39.866 kb on - strand, within Dshi_3639 at 39.870 kb on + strand, within Dshi_3639 at 39.882 kb on + strand, within Dshi_3639 at 39.887 kb on + strand, within Dshi_3639 at 39.887 kb on + strand, within Dshi_3639 at 39.930 kb on + strand, within Dshi_3639 at 39.941 kb on + strand, within Dshi_3639 at 39.971 kb on + strand, within Dshi_3639 at 39.993 kb on + strand, within Dshi_3639 at 40.030 kb on - strand, within Dshi_3639 at 40.030 kb on - strand, within Dshi_3639
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0006 mg/ml remove 37,786 - +0.3 37,786 - -0.0 37,786 - -0.5 37,794 - +0.3 37,811 + -0.8 37,811 + -0.1 37,811 + -0.9 37,819 - +0.4 37,819 - +1.8 37,819 - -0.2 37,821 - +0.2 37,821 - -0.0 37,826 + +0.3 37,826 + +0.4 37,826 + -0.0 37,829 - -0.6 37,829 - -0.3 37,834 - +0.7 37,834 - +0.3 37,834 - +0.1 37,879 + +0.4 37,885 + +0.3 37,907 + -0.1 37,972 - Dshi_3635 0.14 +1.1 37,972 - Dshi_3635 0.14 +1.8 37,982 + Dshi_3635 0.18 +0.0 37,990 - Dshi_3635 0.21 +0.3 37,992 + Dshi_3635 0.22 -0.4 37,992 + Dshi_3635 0.22 -0.3 37,992 + Dshi_3635 0.22 -0.3 37,992 + Dshi_3635 0.22 +1.3 37,992 + Dshi_3635 0.22 -0.7 37,992 + Dshi_3635 0.22 +0.3 37,992 + Dshi_3635 0.22 -0.5 37,992 + Dshi_3635 0.22 +1.1 37,992 + Dshi_3635 0.22 +0.1 37,992 + Dshi_3635 0.22 -0.9 38,000 - Dshi_3635 0.25 -0.2 38,000 - Dshi_3635 0.25 -1.6 38,000 - Dshi_3635 0.25 -0.2 38,000 - Dshi_3635 0.25 -0.2 38,000 - Dshi_3635 0.25 +0.1 38,000 - Dshi_3635 0.25 -0.1 38,000 - Dshi_3635 0.25 +0.6 38,019 - Dshi_3635 0.32 +0.0 38,092 - Dshi_3635 0.60 -0.2 38,093 - Dshi_3635 0.61 +1.1 38,107 + Dshi_3635 0.66 -0.2 38,107 + Dshi_3635 0.66 -0.4 38,113 + Dshi_3635 0.69 +0.2 38,115 - Dshi_3635 0.69 +0.1 38,115 - Dshi_3635 0.69 +0.7 38,118 + Dshi_3635 0.71 +0.3 38,145 + Dshi_3635 0.81 -1.4 38,148 - Dshi_3635 0.82 -0.0 38,153 - Dshi_3635 0.84 +0.2 38,167 - Dshi_3635 0.90 -0.7 38,277 + -0.1 38,286 + +0.4 38,288 + +1.5 38,294 + -0.7 38,294 + +0.0 38,296 - -0.1 38,298 + +0.3 38,302 - +0.1 38,302 - +1.2 38,345 + -1.0 38,358 - Dshi_3636 0.14 -0.1 38,362 + Dshi_3636 0.15 +1.3 38,364 + Dshi_3636 0.16 -1.5 38,371 + Dshi_3636 0.18 -1.0 38,380 + Dshi_3636 0.21 +0.1 38,406 - Dshi_3636 0.30 -0.5 38,406 - Dshi_3636 0.30 -0.0 38,406 - Dshi_3636 0.30 -0.1 38,417 + Dshi_3636 0.34 +0.1 38,430 - Dshi_3636 0.38 +0.0 38,432 + Dshi_3636 0.39 -0.3 38,432 + Dshi_3636 0.39 -0.5 38,432 + Dshi_3636 0.39 +0.5 38,432 + Dshi_3636 0.39 -0.5 38,432 + Dshi_3636 0.39 -0.1 38,440 + Dshi_3636 0.41 +0.3 38,456 + Dshi_3636 0.47 -0.1 38,472 - Dshi_3636 0.52 -0.8 38,474 - Dshi_3636 0.53 -0.1 38,520 + Dshi_3636 0.69 -0.6 38,537 + Dshi_3636 0.74 +0.1 38,541 + Dshi_3636 0.76 +0.3 38,570 + Dshi_3636 0.86 +0.3 38,572 - Dshi_3636 0.86 +0.7 38,588 - -1.2 38,590 - -0.2 38,590 - -0.4 38,613 + +0.5 38,615 + +1.1 38,623 - -0.2 38,636 + -2.0 38,644 - -0.9 38,651 + +0.1 38,651 + -0.5 38,741 + -2.4 38,741 + -1.3 38,741 + -1.9 38,783 - -0.2 38,785 - -1.7 38,913 + Dshi_3637 0.38 +2.3 39,011 + Dshi_3637 0.65 +0.5 39,028 + Dshi_3637 0.70 -2.4 39,042 + Dshi_3637 0.74 -2.7 39,047 + Dshi_3637 0.75 -0.3 39,069 + Dshi_3637 0.82 -0.0 39,077 - Dshi_3637 0.84 -1.3 39,116 - -1.2 39,188 - -0.1 39,188 - +0.3 39,194 - -0.8 39,227 + -2.4 39,228 - +0.8 39,240 - Dshi_3638 0.10 -0.7 39,256 + Dshi_3638 0.13 +0.7 39,274 - Dshi_3638 0.16 -0.5 39,308 + Dshi_3638 0.21 -1.4 39,312 + Dshi_3638 0.22 +0.0 39,320 - Dshi_3638 0.23 -0.0 39,334 + Dshi_3638 0.25 -0.7 39,345 - Dshi_3638 0.27 -0.0 39,360 + Dshi_3638 0.29 -0.9 39,360 + Dshi_3638 0.29 -0.0 39,360 + Dshi_3638 0.29 +0.3 39,368 - Dshi_3638 0.31 -0.5 39,373 - Dshi_3638 0.32 +0.7 39,373 - Dshi_3638 0.32 -0.2 39,380 + Dshi_3638 0.33 +0.3 39,386 - Dshi_3638 0.34 -0.2 39,395 + Dshi_3638 0.35 +0.2 39,402 + Dshi_3638 0.36 +0.5 39,402 + Dshi_3638 0.36 -0.6 39,407 + Dshi_3638 0.37 +0.4 39,410 - Dshi_3638 0.38 +0.3 39,410 - Dshi_3638 0.38 +0.4 39,410 - Dshi_3638 0.38 -0.6 39,426 + Dshi_3638 0.40 -0.2 39,426 + Dshi_3638 0.40 -0.5 39,426 + Dshi_3638 0.40 -1.4 39,430 - Dshi_3638 0.41 +0.4 39,434 - Dshi_3638 0.41 +0.1 39,434 - Dshi_3638 0.41 -0.4 39,434 - Dshi_3638 0.41 -0.2 39,434 - Dshi_3638 0.41 +1.3 39,434 - Dshi_3638 0.41 +0.9 39,434 - Dshi_3638 0.41 -0.1 39,434 - Dshi_3638 0.41 -1.0 39,434 - Dshi_3638 0.41 -0.9 39,434 - Dshi_3638 0.41 +0.2 39,444 - Dshi_3638 0.43 +0.1 39,460 + Dshi_3638 0.46 +0.4 39,473 - Dshi_3638 0.48 -1.3 39,494 - Dshi_3638 0.51 +1.3 39,507 - Dshi_3638 0.53 +0.0 39,521 - Dshi_3638 0.55 +1.0 39,554 + Dshi_3638 0.61 -0.9 39,556 - Dshi_3638 0.61 -0.3 39,556 - Dshi_3638 0.61 -0.3 39,585 - Dshi_3638 0.66 -0.1 39,600 - Dshi_3638 0.68 +0.0 39,602 - Dshi_3638 0.68 +0.3 39,668 - Dshi_3638 0.79 -2.2 39,682 + Dshi_3638 0.81 -0.6 39,697 - Dshi_3638 0.84 +0.1 39,715 + Dshi_3638 0.87 -0.0 39,716 - Dshi_3638 0.87 -0.2 39,735 + Dshi_3638 0.90 +0.3 39,735 + Dshi_3638 0.90 -0.8 39,735 + Dshi_3638 0.90 +0.5 39,742 + -0.1 39,743 - -0.4 39,743 - -0.1 39,743 - -0.1 39,743 - -0.6 39,743 - +1.1 39,748 - +0.6 39,779 - -0.4 39,811 + -0.4 39,848 + Dshi_3639 0.18 -0.8 39,858 + Dshi_3639 0.22 -0.3 39,858 + Dshi_3639 0.22 -0.3 39,858 + Dshi_3639 0.22 +0.1 39,866 - Dshi_3639 0.24 -0.5 39,866 - Dshi_3639 0.24 +0.0 39,866 - Dshi_3639 0.24 +0.2 39,866 - Dshi_3639 0.24 +0.5 39,866 - Dshi_3639 0.24 -0.4 39,870 + Dshi_3639 0.26 +0.7 39,882 + Dshi_3639 0.30 +0.9 39,887 + Dshi_3639 0.32 +0.4 39,887 + Dshi_3639 0.32 -0.5 39,930 + Dshi_3639 0.46 +0.1 39,941 + Dshi_3639 0.50 -1.4 39,971 + Dshi_3639 0.60 +0.9 39,993 + Dshi_3639 0.68 -0.8 40,030 - Dshi_3639 0.80 -0.3 40,030 - Dshi_3639 0.80 -0.1
Or see this region's nucleotide sequence