Experiment: MoYLS4 with FCCP 10uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trmE and DVU1080 are separated by 318 nucleotides DVU1080 and DVU1081 overlap by 4 nucleotides DVU1081 and DVU1082 are separated by 116 nucleotides DVU1082 and phoB are separated by 173 nucleotides
DVU1079: trmE - tRNA modification GTPase TrmE (TIGR), at 1,182,378 to 1,183,751
trmE
DVU1080: DVU1080 - iron-sulfur cluster-binding protein (TIGR), at 1,184,070 to 1,184,612
DVU1080
DVU1081: DVU1081 - iron-sulfur cluster-binding protein (TIGR), at 1,184,609 to 1,186,228
DVU1081
DVU1082: DVU1082 - 3- 5 exonuclease domain protein (TIGR), at 1,186,345 to 1,186,953
DVU1082
DVU1083: phoB - phosphate regulon transcriptional regulator PhoB (TIGR), at 1,187,127 to 1,187,816
phoB
Position (kb)
1184
1185
1186
1187 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1183.679 kb on - strand at 1183.949 kb on - strand at 1183.949 kb on - strand at 1184.102 kb on - strand at 1184.212 kb on + strand, within DVU1080 at 1184.218 kb on - strand, within DVU1080 at 1184.243 kb on + strand, within DVU1080 at 1184.583 kb on + strand at 1184.597 kb on + strand at 1184.597 kb on + strand at 1184.605 kb on + strand at 1184.605 kb on - strand at 1184.605 kb on - strand at 1184.605 kb on - strand at 1184.605 kb on - strand at 1184.605 kb on - strand at 1184.681 kb on - strand at 1184.691 kb on - strand at 1184.725 kb on + strand at 1184.725 kb on + strand at 1184.733 kb on - strand at 1184.759 kb on + strand at 1184.888 kb on + strand, within DVU1081 at 1184.888 kb on + strand, within DVU1081 at 1184.891 kb on - strand, within DVU1081 at 1184.967 kb on + strand, within DVU1081 at 1184.975 kb on - strand, within DVU1081 at 1185.001 kb on - strand, within DVU1081 at 1185.030 kb on - strand, within DVU1081 at 1185.046 kb on - strand, within DVU1081 at 1185.057 kb on + strand, within DVU1081 at 1185.070 kb on + strand, within DVU1081 at 1185.166 kb on - strand, within DVU1081 at 1185.178 kb on + strand, within DVU1081 at 1185.199 kb on - strand, within DVU1081 at 1185.230 kb on - strand, within DVU1081 at 1185.245 kb on + strand, within DVU1081 at 1185.250 kb on + strand, within DVU1081 at 1185.258 kb on - strand, within DVU1081 at 1185.319 kb on + strand, within DVU1081 at 1185.348 kb on - strand, within DVU1081 at 1185.355 kb on + strand, within DVU1081 at 1185.392 kb on + strand, within DVU1081 at 1185.576 kb on + strand, within DVU1081 at 1185.591 kb on + strand, within DVU1081 at 1185.619 kb on + strand, within DVU1081 at 1185.627 kb on - strand, within DVU1081 at 1185.707 kb on + strand, within DVU1081 at 1185.707 kb on + strand, within DVU1081 at 1185.717 kb on - strand, within DVU1081 at 1185.738 kb on + strand, within DVU1081 at 1185.797 kb on + strand, within DVU1081 at 1185.857 kb on + strand, within DVU1081 at 1185.933 kb on + strand, within DVU1081 at 1185.933 kb on + strand, within DVU1081 at 1185.941 kb on - strand, within DVU1081 at 1186.018 kb on + strand, within DVU1081 at 1186.085 kb on - strand at 1186.487 kb on + strand, within DVU1082 at 1186.621 kb on - strand, within DVU1082 at 1186.835 kb on + strand, within DVU1082 at 1186.843 kb on - strand, within DVU1082 at 1186.858 kb on - strand, within DVU1082 at 1187.052 kb on + strand at 1187.064 kb on + strand at 1187.118 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with FCCP 10uM remove 1,183,679 - -0.9 1,183,949 - +0.1 1,183,949 - +0.5 1,184,102 - -0.2 1,184,212 + DVU1080 0.26 +0.7 1,184,218 - DVU1080 0.27 -0.3 1,184,243 + DVU1080 0.32 -0.3 1,184,583 + -0.6 1,184,597 + -0.5 1,184,597 + -0.5 1,184,605 + -0.4 1,184,605 - -0.2 1,184,605 - -1.9 1,184,605 - -1.3 1,184,605 - +0.7 1,184,605 - +0.7 1,184,681 - -0.4 1,184,691 - +0.1 1,184,725 + +0.4 1,184,725 + -0.1 1,184,733 - -0.1 1,184,759 + +0.1 1,184,888 + DVU1081 0.17 -1.9 1,184,888 + DVU1081 0.17 -0.1 1,184,891 - DVU1081 0.17 +1.1 1,184,967 + DVU1081 0.22 +0.7 1,184,975 - DVU1081 0.23 +0.1 1,185,001 - DVU1081 0.24 +0.7 1,185,030 - DVU1081 0.26 -0.0 1,185,046 - DVU1081 0.27 +0.7 1,185,057 + DVU1081 0.28 +0.8 1,185,070 + DVU1081 0.28 +0.2 1,185,166 - DVU1081 0.34 +0.3 1,185,178 + DVU1081 0.35 -0.2 1,185,199 - DVU1081 0.36 +0.7 1,185,230 - DVU1081 0.38 -0.4 1,185,245 + DVU1081 0.39 -0.1 1,185,250 + DVU1081 0.40 +0.4 1,185,258 - DVU1081 0.40 -1.3 1,185,319 + DVU1081 0.44 +1.2 1,185,348 - DVU1081 0.46 -0.3 1,185,355 + DVU1081 0.46 -0.3 1,185,392 + DVU1081 0.48 -1.3 1,185,576 + DVU1081 0.60 -0.2 1,185,591 + DVU1081 0.61 -1.5 1,185,619 + DVU1081 0.62 -0.7 1,185,627 - DVU1081 0.63 -0.3 1,185,707 + DVU1081 0.68 +0.3 1,185,707 + DVU1081 0.68 +0.4 1,185,717 - DVU1081 0.68 +1.2 1,185,738 + DVU1081 0.70 +1.7 1,185,797 + DVU1081 0.73 +0.7 1,185,857 + DVU1081 0.77 +0.7 1,185,933 + DVU1081 0.82 -0.2 1,185,933 + DVU1081 0.82 +1.2 1,185,941 - DVU1081 0.82 -0.4 1,186,018 + DVU1081 0.87 +1.9 1,186,085 - +1.4 1,186,487 + DVU1082 0.23 +0.3 1,186,621 - DVU1082 0.45 -0.3 1,186,835 + DVU1082 0.80 -0.9 1,186,843 - DVU1082 0.82 -0.5 1,186,858 - DVU1082 0.84 +0.0 1,187,052 + +0.0 1,187,064 + -0.9 1,187,118 + +0.7
Or see this region's nucleotide sequence