Experiment: MoYLS4 with FCCP 10uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaG and DVU0091 are separated by 52 nucleotides DVU0091 and DVU0092 are separated by 56 nucleotides DVU0092 and DVU0093 are separated by 255 nucleotides
DVU0090: wcaG - GDP-fucose synthetase (TIGR), at 115,316 to 116,287
wcaG
DVU0091: DVU0091 - conserved hypothetical protein (TIGR), at 116,340 to 117,062
DVU0091
DVU0092: DVU0092 - sensory box histidine kinase (TIGR), at 117,119 to 120,436
DVU0092
DVU0093: DVU0093 - conserved domain protein/glycosyl transferase, group 1 family protein (TIGR), at 120,692 to 123,142
DVU0093
Position (kb)
117
118
119
120
121 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 116.226 kb on + strand at 116.270 kb on + strand at 116.273 kb on - strand at 116.282 kb on + strand at 116.290 kb on - strand at 116.508 kb on - strand, within DVU0091 at 116.535 kb on - strand, within DVU0091 at 116.609 kb on + strand, within DVU0091 at 116.617 kb on - strand, within DVU0091 at 116.617 kb on - strand, within DVU0091 at 116.687 kb on - strand, within DVU0091 at 116.687 kb on - strand, within DVU0091 at 116.696 kb on + strand, within DVU0091 at 116.714 kb on - strand, within DVU0091 at 116.714 kb on - strand, within DVU0091 at 116.724 kb on + strand, within DVU0091 at 116.764 kb on - strand, within DVU0091 at 116.896 kb on - strand, within DVU0091 at 116.915 kb on + strand, within DVU0091 at 116.939 kb on + strand, within DVU0091 at 117.224 kb on + strand at 117.224 kb on + strand at 117.289 kb on + strand at 117.314 kb on + strand at 117.329 kb on - strand at 117.361 kb on + strand at 117.378 kb on + strand at 117.573 kb on + strand, within DVU0092 at 117.750 kb on - strand, within DVU0092 at 117.804 kb on + strand, within DVU0092 at 117.805 kb on - strand, within DVU0092 at 117.812 kb on - strand, within DVU0092 at 117.812 kb on - strand, within DVU0092 at 117.850 kb on - strand, within DVU0092 at 117.908 kb on - strand, within DVU0092 at 118.012 kb on - strand, within DVU0092 at 118.022 kb on + strand, within DVU0092 at 118.030 kb on - strand, within DVU0092 at 118.081 kb on + strand, within DVU0092 at 118.084 kb on - strand, within DVU0092 at 118.094 kb on - strand, within DVU0092 at 118.128 kb on - strand, within DVU0092 at 118.182 kb on + strand, within DVU0092 at 118.190 kb on - strand, within DVU0092 at 118.349 kb on + strand, within DVU0092 at 118.352 kb on - strand, within DVU0092 at 118.357 kb on - strand, within DVU0092 at 118.397 kb on - strand, within DVU0092 at 118.505 kb on - strand, within DVU0092 at 118.510 kb on - strand, within DVU0092 at 118.610 kb on + strand, within DVU0092 at 118.652 kb on + strand, within DVU0092 at 118.660 kb on - strand, within DVU0092 at 118.724 kb on + strand, within DVU0092 at 118.789 kb on + strand, within DVU0092 at 118.810 kb on + strand, within DVU0092 at 118.929 kb on + strand, within DVU0092 at 118.976 kb on + strand, within DVU0092 at 119.051 kb on - strand, within DVU0092 at 119.118 kb on - strand, within DVU0092 at 119.140 kb on + strand, within DVU0092 at 119.240 kb on + strand, within DVU0092 at 119.245 kb on + strand, within DVU0092 at 119.253 kb on - strand, within DVU0092 at 119.253 kb on - strand, within DVU0092 at 119.309 kb on + strand, within DVU0092 at 119.312 kb on - strand, within DVU0092 at 119.312 kb on - strand, within DVU0092 at 119.344 kb on + strand, within DVU0092 at 119.453 kb on + strand, within DVU0092 at 119.607 kb on - strand, within DVU0092 at 119.607 kb on - strand, within DVU0092 at 119.624 kb on - strand, within DVU0092 at 119.674 kb on - strand, within DVU0092 at 119.701 kb on + strand, within DVU0092 at 119.709 kb on - strand, within DVU0092 at 119.776 kb on - strand, within DVU0092 at 119.952 kb on + strand, within DVU0092 at 119.960 kb on - strand, within DVU0092 at 119.986 kb on + strand, within DVU0092 at 120.017 kb on + strand, within DVU0092 at 120.047 kb on + strand, within DVU0092 at 120.055 kb on - strand, within DVU0092 at 120.067 kb on - strand, within DVU0092 at 120.079 kb on + strand, within DVU0092 at 120.086 kb on + strand, within DVU0092 at 120.094 kb on - strand, within DVU0092 at 120.108 kb on + strand at 120.196 kb on + strand at 120.201 kb on + strand at 120.209 kb on - strand at 120.209 kb on - strand at 120.209 kb on - strand at 120.224 kb on - strand at 120.311 kb on + strand at 120.319 kb on - strand at 120.401 kb on + strand at 120.401 kb on + strand at 120.418 kb on + strand at 120.426 kb on - strand at 120.465 kb on + strand at 120.486 kb on + strand at 120.486 kb on + strand at 120.560 kb on - strand at 120.580 kb on + strand at 120.588 kb on - strand at 120.688 kb on - strand at 120.713 kb on - strand at 120.718 kb on - strand at 120.728 kb on + strand at 120.818 kb on + strand at 120.833 kb on - strand at 120.905 kb on - strand at 120.936 kb on + strand at 121.068 kb on - strand, within DVU0093 at 121.111 kb on + strand, within DVU0093 at 121.177 kb on - strand, within DVU0093 at 121.208 kb on - strand, within DVU0093 at 121.212 kb on + strand, within DVU0093 at 121.247 kb on - strand, within DVU0093 at 121.269 kb on - strand, within DVU0093 at 121.399 kb on - strand, within DVU0093
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with FCCP 10uM remove 116,226 + +0.3 116,270 + -0.2 116,273 - -1.5 116,282 + -0.1 116,290 - -1.4 116,508 - DVU0091 0.23 +3.1 116,535 - DVU0091 0.27 -0.8 116,609 + DVU0091 0.37 -0.8 116,617 - DVU0091 0.38 -0.3 116,617 - DVU0091 0.38 -0.3 116,687 - DVU0091 0.48 +0.2 116,687 - DVU0091 0.48 -0.5 116,696 + DVU0091 0.49 -0.3 116,714 - DVU0091 0.52 -2.0 116,714 - DVU0091 0.52 +0.8 116,724 + DVU0091 0.53 -0.5 116,764 - DVU0091 0.59 +0.4 116,896 - DVU0091 0.77 -0.1 116,915 + DVU0091 0.80 +0.8 116,939 + DVU0091 0.83 +0.8 117,224 + -1.2 117,224 + +0.2 117,289 + -0.3 117,314 + -3.2 117,329 - +0.2 117,361 + +0.8 117,378 + -0.4 117,573 + DVU0092 0.14 -0.2 117,750 - DVU0092 0.19 -0.8 117,804 + DVU0092 0.21 -1.2 117,805 - DVU0092 0.21 -1.3 117,812 - DVU0092 0.21 +0.8 117,812 - DVU0092 0.21 -1.8 117,850 - DVU0092 0.22 -0.9 117,908 - DVU0092 0.24 -1.5 118,012 - DVU0092 0.27 -2.0 118,022 + DVU0092 0.27 -0.2 118,030 - DVU0092 0.27 -1.1 118,081 + DVU0092 0.29 -1.3 118,084 - DVU0092 0.29 -0.2 118,094 - DVU0092 0.29 +0.1 118,128 - DVU0092 0.30 -0.3 118,182 + DVU0092 0.32 -2.0 118,190 - DVU0092 0.32 +0.8 118,349 + DVU0092 0.37 -2.6 118,352 - DVU0092 0.37 -1.2 118,357 - DVU0092 0.37 -1.6 118,397 - DVU0092 0.39 +0.0 118,505 - DVU0092 0.42 -0.5 118,510 - DVU0092 0.42 +0.1 118,610 + DVU0092 0.45 -0.2 118,652 + DVU0092 0.46 +0.4 118,660 - DVU0092 0.46 -0.4 118,724 + DVU0092 0.48 -1.0 118,789 + DVU0092 0.50 -0.2 118,810 + DVU0092 0.51 -0.8 118,929 + DVU0092 0.55 -1.0 118,976 + DVU0092 0.56 +1.8 119,051 - DVU0092 0.58 -0.8 119,118 - DVU0092 0.60 -0.8 119,140 + DVU0092 0.61 -1.1 119,240 + DVU0092 0.64 +0.5 119,245 + DVU0092 0.64 +0.1 119,253 - DVU0092 0.64 +0.8 119,253 - DVU0092 0.64 -1.0 119,309 + DVU0092 0.66 -1.6 119,312 - DVU0092 0.66 -1.0 119,312 - DVU0092 0.66 -0.6 119,344 + DVU0092 0.67 -1.6 119,453 + DVU0092 0.70 -1.1 119,607 - DVU0092 0.75 +0.8 119,607 - DVU0092 0.75 -0.2 119,624 - DVU0092 0.75 -0.8 119,674 - DVU0092 0.77 -0.2 119,701 + DVU0092 0.78 -0.8 119,709 - DVU0092 0.78 -1.1 119,776 - DVU0092 0.80 -1.1 119,952 + DVU0092 0.85 -0.9 119,960 - DVU0092 0.86 -0.2 119,986 + DVU0092 0.86 -1.5 120,017 + DVU0092 0.87 -1.4 120,047 + DVU0092 0.88 -0.5 120,055 - DVU0092 0.88 -0.5 120,067 - DVU0092 0.89 -1.5 120,079 + DVU0092 0.89 +0.2 120,086 + DVU0092 0.89 -1.2 120,094 - DVU0092 0.90 -0.8 120,108 + +0.8 120,196 + -2.2 120,201 + -1.5 120,209 - -1.5 120,209 - -0.7 120,209 - -1.5 120,224 - -1.0 120,311 + -0.8 120,319 - -0.8 120,401 + -3.0 120,401 + -1.0 120,418 + -0.3 120,426 - -0.1 120,465 + +0.8 120,486 + -2.2 120,486 + -0.9 120,560 - +1.6 120,580 + -0.2 120,588 - -0.9 120,688 - -0.5 120,713 - -0.5 120,718 - -0.2 120,728 + -0.8 120,818 + -0.4 120,833 - +0.9 120,905 - +0.2 120,936 + -0.2 121,068 - DVU0093 0.15 +0.0 121,111 + DVU0093 0.17 -0.2 121,177 - DVU0093 0.20 -1.0 121,208 - DVU0093 0.21 -0.2 121,212 + DVU0093 0.21 +1.2 121,247 - DVU0093 0.23 -1.3 121,269 - DVU0093 0.24 -0.4 121,399 - DVU0093 0.29 +2.4
Or see this region's nucleotide sequence