Experiment: m.b. Cisplatin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c08940 and PGA1_c08950 are separated by 232 nucleotides PGA1_c08950 and PGA1_c08960 are separated by 426 nucleotides PGA1_c08960 and PGA1_c08970 are separated by 19 nucleotides PGA1_c08970 and PGA1_c08980 are separated by 205 nucleotides PGA1_c08980 and PGA1_c08990 are separated by 142 nucleotides PGA1_c08990 and PGA1_c09000 overlap by 2 nucleotides
PGA1_c08940: PGA1_c08940 - N utilization substance protein B homolog, at 923,710 to 924,201
_c08940
PGA1_c08950: PGA1_c08950 - hypothetical protein, at 924,434 to 924,763
_c08950
PGA1_c08960: PGA1_c08960 - DNA repair endonuclease mmcB (DUF1052) <small>(from data)</small>, at 925,190 to 925,645
_c08960
PGA1_c08970: PGA1_c08970 - hypothetical protein, at 925,665 to 925,853
_c08970
PGA1_c08980: PGA1_c08980 - tRNA-His, at 926,059 to 926,135
_c08980
PGA1_c08990: PGA1_c08990 - hypothetical protein, at 926,278 to 926,592
_c08990
PGA1_c09000: PGA1_c09000 - ribosomal large subunit pseudouridine synthase D, at 926,591 to 927,625
_c09000
Position (kb) 925
926 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 924.224 kb on + strand at 924.224 kb on + strand at 924.224 kb on + strand at 924.227 kb on + strand at 924.229 kb on + strand at 924.232 kb on - strand at 924.232 kb on - strand at 924.232 kb on - strand at 924.232 kb on - strand at 924.237 kb on + strand at 924.237 kb on - strand at 924.237 kb on - strand at 924.245 kb on - strand at 924.282 kb on + strand at 924.283 kb on - strand at 924.417 kb on + strand at 924.502 kb on - strand, within PGA1_c08950 at 924.552 kb on - strand, within PGA1_c08950 at 924.581 kb on + strand, within PGA1_c08950 at 924.591 kb on - strand, within PGA1_c08950 at 924.706 kb on + strand, within PGA1_c08950 at 924.947 kb on - strand at 925.023 kb on + strand at 925.137 kb on + strand at 925.202 kb on - strand at 925.206 kb on + strand at 925.214 kb on - strand at 925.248 kb on - strand, within PGA1_c08960 at 925.248 kb on - strand, within PGA1_c08960 at 925.258 kb on - strand, within PGA1_c08960 at 925.321 kb on + strand, within PGA1_c08960 at 925.333 kb on + strand, within PGA1_c08960 at 925.338 kb on + strand, within PGA1_c08960 at 925.346 kb on - strand, within PGA1_c08960 at 925.348 kb on + strand, within PGA1_c08960 at 925.348 kb on + strand, within PGA1_c08960 at 925.393 kb on + strand, within PGA1_c08960 at 925.397 kb on + strand, within PGA1_c08960 at 925.398 kb on + strand, within PGA1_c08960 at 925.401 kb on - strand, within PGA1_c08960 at 925.401 kb on - strand, within PGA1_c08960 at 925.406 kb on - strand, within PGA1_c08960 at 925.433 kb on + strand, within PGA1_c08960 at 925.472 kb on + strand, within PGA1_c08960 at 925.625 kb on + strand at 925.632 kb on - strand at 925.633 kb on - strand at 925.699 kb on + strand, within PGA1_c08970 at 925.840 kb on + strand at 925.873 kb on - strand at 925.880 kb on - strand at 925.901 kb on + strand at 925.929 kb on + strand at 925.944 kb on + strand at 925.952 kb on - strand at 925.952 kb on - strand at 925.963 kb on - strand at 925.963 kb on - strand at 926.233 kb on + strand at 926.243 kb on - strand at 926.243 kb on - strand at 926.243 kb on - strand at 926.243 kb on - strand at 926.243 kb on - strand at 926.243 kb on - strand at 926.243 kb on - strand at 926.293 kb on - strand at 926.293 kb on - strand at 926.340 kb on - strand, within PGA1_c08990 at 926.439 kb on + strand, within PGA1_c08990 at 926.484 kb on - strand, within PGA1_c08990 at 926.484 kb on - strand, within PGA1_c08990 at 926.596 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cisplatin 0.025 mg/ml remove 924,224 + +0.4 924,224 + +1.3 924,224 + +1.2 924,227 + -1.0 924,229 + +0.9 924,232 - -0.6 924,232 - +1.2 924,232 - -2.1 924,232 - -0.7 924,237 + +0.0 924,237 - +1.0 924,237 - +0.0 924,245 - +0.0 924,282 + +0.9 924,283 - -0.3 924,417 + -1.1 924,502 - PGA1_c08950 0.21 +0.1 924,552 - PGA1_c08950 0.36 +0.9 924,581 + PGA1_c08950 0.45 +0.1 924,591 - PGA1_c08950 0.48 +0.3 924,706 + PGA1_c08950 0.82 +0.4 924,947 - +1.1 925,023 + +2.6 925,137 + +0.6 925,202 - -2.3 925,206 + -4.0 925,214 - -0.1 925,248 - PGA1_c08960 0.13 -1.5 925,248 - PGA1_c08960 0.13 -0.1 925,258 - PGA1_c08960 0.15 -2.3 925,321 + PGA1_c08960 0.29 -2.7 925,333 + PGA1_c08960 0.31 -1.1 925,338 + PGA1_c08960 0.32 -1.5 925,346 - PGA1_c08960 0.34 -1.9 925,348 + PGA1_c08960 0.35 -1.1 925,348 + PGA1_c08960 0.35 -2.8 925,393 + PGA1_c08960 0.45 -2.6 925,397 + PGA1_c08960 0.45 -0.8 925,398 + PGA1_c08960 0.46 -2.6 925,401 - PGA1_c08960 0.46 -0.7 925,401 - PGA1_c08960 0.46 -0.9 925,406 - PGA1_c08960 0.47 -1.8 925,433 + PGA1_c08960 0.53 -1.1 925,472 + PGA1_c08960 0.62 -3.4 925,625 + -1.0 925,632 - +0.4 925,633 - -1.3 925,699 + PGA1_c08970 0.18 -0.6 925,840 + -0.6 925,873 - +1.1 925,880 - -0.5 925,901 + +0.3 925,929 + +1.2 925,944 + +0.1 925,952 - +0.7 925,952 - -2.5 925,963 - +1.4 925,963 - +0.8 926,233 + +0.7 926,243 - +1.6 926,243 - +0.8 926,243 - -0.1 926,243 - +0.9 926,243 - -0.4 926,243 - +1.3 926,243 - -0.4 926,293 - +0.4 926,293 - +0.5 926,340 - PGA1_c08990 0.20 +0.6 926,439 + PGA1_c08990 0.51 -1.6 926,484 - PGA1_c08990 0.65 -0.7 926,484 - PGA1_c08990 0.65 -0.6 926,596 - -1.7
Or see this region's nucleotide sequence