Experiment: m.b. Cisplatin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c00020 and PGA1_c00030 are separated by 97 nucleotides PGA1_c00030 and PGA1_c00040 overlap by 4 nucleotides PGA1_c00040 and PGA1_c00050 are separated by 14 nucleotides
PGA1_c00020: PGA1_c00020 - DNA polymerase III subunit beta, at 1,838 to 2,959
_c00020
PGA1_c00030: PGA1_c00030 - DNA replication and repair protein RecF, at 3,057 to 4,154
_c00030
PGA1_c00040: PGA1_c00040 - putative LysE type translocator, at 4,151 to 4,771
_c00040
PGA1_c00050: PGA1_c00050 - putative transcriptional regulator, tetR family, at 4,786 to 5,385
_c00050
Position (kb)
3
4
5 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2.953 kb on + strand at 2.953 kb on + strand at 2.953 kb on + strand at 2.961 kb on - strand at 2.963 kb on + strand at 2.969 kb on + strand at 2.990 kb on + strand at 2.990 kb on + strand at 2.998 kb on - strand at 2.998 kb on - strand at 3.053 kb on + strand at 3.070 kb on - strand at 3.073 kb on + strand at 3.073 kb on + strand at 3.093 kb on + strand at 3.131 kb on + strand at 3.248 kb on + strand, within PGA1_c00030 at 3.281 kb on + strand, within PGA1_c00030 at 3.289 kb on + strand, within PGA1_c00030 at 3.317 kb on - strand, within PGA1_c00030 at 3.342 kb on - strand, within PGA1_c00030 at 3.386 kb on - strand, within PGA1_c00030 at 3.510 kb on + strand, within PGA1_c00030 at 3.584 kb on - strand, within PGA1_c00030 at 3.640 kb on + strand, within PGA1_c00030 at 3.674 kb on - strand, within PGA1_c00030 at 3.707 kb on - strand, within PGA1_c00030 at 3.732 kb on + strand, within PGA1_c00030 at 3.750 kb on - strand, within PGA1_c00030 at 3.763 kb on + strand, within PGA1_c00030 at 3.799 kb on + strand, within PGA1_c00030 at 3.807 kb on - strand, within PGA1_c00030 at 3.807 kb on - strand, within PGA1_c00030 at 3.831 kb on - strand, within PGA1_c00030 at 3.848 kb on + strand, within PGA1_c00030 at 3.848 kb on + strand, within PGA1_c00030 at 3.897 kb on + strand, within PGA1_c00030 at 4.009 kb on - strand, within PGA1_c00030 at 4.035 kb on - strand, within PGA1_c00030 at 4.046 kb on + strand at 4.066 kb on + strand at 4.066 kb on + strand at 4.066 kb on + strand at 4.066 kb on + strand at 4.074 kb on - strand at 4.074 kb on - strand at 4.074 kb on - strand at 4.098 kb on + strand at 4.103 kb on + strand at 4.111 kb on + strand at 4.114 kb on - strand at 4.132 kb on + strand at 4.195 kb on - strand at 4.212 kb on - strand at 4.235 kb on - strand, within PGA1_c00040 at 4.290 kb on - strand, within PGA1_c00040 at 4.369 kb on + strand, within PGA1_c00040 at 4.402 kb on - strand, within PGA1_c00040 at 4.480 kb on + strand, within PGA1_c00040 at 4.522 kb on - strand, within PGA1_c00040 at 4.580 kb on + strand, within PGA1_c00040 at 4.580 kb on + strand, within PGA1_c00040 at 4.581 kb on - strand, within PGA1_c00040 at 4.656 kb on - strand, within PGA1_c00040 at 4.656 kb on - strand, within PGA1_c00040 at 4.666 kb on - strand, within PGA1_c00040 at 4.671 kb on - strand, within PGA1_c00040 at 4.777 kb on + strand at 4.780 kb on - strand at 4.780 kb on - strand at 4.785 kb on + strand at 4.825 kb on + strand at 4.858 kb on + strand, within PGA1_c00050 at 4.873 kb on + strand, within PGA1_c00050 at 4.913 kb on + strand, within PGA1_c00050 at 4.921 kb on - strand, within PGA1_c00050 at 4.921 kb on - strand, within PGA1_c00050 at 4.950 kb on + strand, within PGA1_c00050 at 4.971 kb on + strand, within PGA1_c00050 at 4.979 kb on - strand, within PGA1_c00050 at 5.031 kb on - strand, within PGA1_c00050 at 5.057 kb on - strand, within PGA1_c00050 at 5.068 kb on - strand, within PGA1_c00050 at 5.103 kb on + strand, within PGA1_c00050 at 5.148 kb on - strand, within PGA1_c00050 at 5.152 kb on + strand, within PGA1_c00050
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cisplatin 0.025 mg/ml remove 2,953 + +0.3 2,953 + +0.5 2,953 + +1.1 2,961 - -0.8 2,963 + +0.3 2,969 + -0.7 2,990 + +0.4 2,990 + -1.5 2,998 - -2.3 2,998 - -3.4 3,053 + -0.0 3,070 - -1.5 3,073 + -1.2 3,073 + -2.2 3,093 + -3.7 3,131 + -2.3 3,248 + PGA1_c00030 0.17 -2.9 3,281 + PGA1_c00030 0.20 -1.7 3,289 + PGA1_c00030 0.21 -2.3 3,317 - PGA1_c00030 0.24 -2.9 3,342 - PGA1_c00030 0.26 -0.2 3,386 - PGA1_c00030 0.30 -1.3 3,510 + PGA1_c00030 0.41 -1.7 3,584 - PGA1_c00030 0.48 -1.5 3,640 + PGA1_c00030 0.53 -1.3 3,674 - PGA1_c00030 0.56 -2.0 3,707 - PGA1_c00030 0.59 -2.3 3,732 + PGA1_c00030 0.61 -3.2 3,750 - PGA1_c00030 0.63 -1.9 3,763 + PGA1_c00030 0.64 -2.9 3,799 + PGA1_c00030 0.68 -1.5 3,807 - PGA1_c00030 0.68 -1.7 3,807 - PGA1_c00030 0.68 -1.2 3,831 - PGA1_c00030 0.70 -1.0 3,848 + PGA1_c00030 0.72 -1.5 3,848 + PGA1_c00030 0.72 -1.9 3,897 + PGA1_c00030 0.77 -0.2 4,009 - PGA1_c00030 0.87 -1.5 4,035 - PGA1_c00030 0.89 -2.7 4,046 + -0.7 4,066 + -2.7 4,066 + -2.2 4,066 + -0.5 4,066 + -2.2 4,074 - -1.4 4,074 - +0.6 4,074 - -3.1 4,098 + -0.2 4,103 + -3.7 4,111 + -1.3 4,114 - -3.9 4,132 + +0.5 4,195 - +0.6 4,212 - -3.2 4,235 - PGA1_c00040 0.14 +1.3 4,290 - PGA1_c00040 0.22 +0.3 4,369 + PGA1_c00040 0.35 -0.4 4,402 - PGA1_c00040 0.40 +0.9 4,480 + PGA1_c00040 0.53 +2.4 4,522 - PGA1_c00040 0.60 +0.5 4,580 + PGA1_c00040 0.69 -0.7 4,580 + PGA1_c00040 0.69 -0.3 4,581 - PGA1_c00040 0.69 -0.5 4,656 - PGA1_c00040 0.81 +0.0 4,656 - PGA1_c00040 0.81 -0.3 4,666 - PGA1_c00040 0.83 -0.3 4,671 - PGA1_c00040 0.84 +0.8 4,777 + -0.2 4,780 - +0.1 4,780 - +0.7 4,785 + +0.4 4,825 + -0.7 4,858 + PGA1_c00050 0.12 +0.2 4,873 + PGA1_c00050 0.14 -1.6 4,913 + PGA1_c00050 0.21 -0.2 4,921 - PGA1_c00050 0.23 -1.6 4,921 - PGA1_c00050 0.23 -1.4 4,950 + PGA1_c00050 0.27 -2.5 4,971 + PGA1_c00050 0.31 -0.2 4,979 - PGA1_c00050 0.32 -1.3 5,031 - PGA1_c00050 0.41 -0.4 5,057 - PGA1_c00050 0.45 -0.7 5,068 - PGA1_c00050 0.47 -1.5 5,103 + PGA1_c00050 0.53 -1.5 5,148 - PGA1_c00050 0.60 +0.0 5,152 + PGA1_c00050 0.61 +0.8
Or see this region's nucleotide sequence