Experiment: m.b. Cisplatin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c21250 and PGA1_c21260 are separated by 14 nucleotides PGA1_c21260 and PGA1_c21270 are separated by 250 nucleotides PGA1_c21270 and PGA1_c21280 are separated by 172 nucleotides
PGA1_c21250: PGA1_c21250 - aspartate aminotransferase AatA, at 2,198,608 to 2,199,810
_c21250
PGA1_c21260: PGA1_c21260 - hypothetical protein, at 2,199,825 to 2,200,247
_c21260
PGA1_c21270: PGA1_c21270 - hypothetical protein, at 2,200,498 to 2,201,364
_c21270
PGA1_c21280: PGA1_c21280 - transcriptional regulator, LysR family, at 2,201,537 to 2,202,451
_c21280
Position (kb)
2200
2201
2202 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2199.517 kb on - strand, within PGA1_c21250 at 2199.522 kb on - strand, within PGA1_c21250 at 2199.548 kb on - strand, within PGA1_c21250 at 2199.621 kb on + strand, within PGA1_c21250 at 2199.807 kb on - strand at 2199.807 kb on - strand at 2199.809 kb on + strand at 2199.817 kb on - strand at 2199.817 kb on - strand at 2199.817 kb on - strand at 2199.817 kb on - strand at 2199.842 kb on - strand at 2199.845 kb on - strand at 2199.996 kb on - strand, within PGA1_c21260 at 2200.052 kb on + strand, within PGA1_c21260 at 2200.084 kb on + strand, within PGA1_c21260 at 2200.107 kb on + strand, within PGA1_c21260 at 2200.120 kb on - strand, within PGA1_c21260 at 2200.168 kb on + strand, within PGA1_c21260 at 2200.175 kb on + strand, within PGA1_c21260 at 2200.185 kb on + strand, within PGA1_c21260 at 2200.241 kb on + strand at 2200.241 kb on + strand at 2200.309 kb on + strand at 2200.309 kb on + strand at 2200.317 kb on - strand at 2200.317 kb on - strand at 2200.328 kb on - strand at 2200.340 kb on + strand at 2200.377 kb on - strand at 2200.436 kb on - strand at 2200.436 kb on - strand at 2200.445 kb on + strand at 2200.445 kb on + strand at 2200.445 kb on + strand at 2200.445 kb on + strand at 2200.453 kb on - strand at 2200.453 kb on - strand at 2200.453 kb on - strand at 2200.468 kb on + strand at 2200.470 kb on + strand at 2200.478 kb on - strand at 2200.497 kb on - strand at 2200.534 kb on + strand at 2200.534 kb on + strand at 2200.534 kb on + strand at 2200.539 kb on + strand at 2200.542 kb on - strand at 2200.626 kb on - strand, within PGA1_c21270 at 2200.737 kb on + strand, within PGA1_c21270 at 2200.748 kb on - strand, within PGA1_c21270 at 2200.813 kb on + strand, within PGA1_c21270 at 2200.818 kb on + strand, within PGA1_c21270 at 2200.819 kb on + strand, within PGA1_c21270 at 2200.821 kb on - strand, within PGA1_c21270 at 2200.821 kb on - strand, within PGA1_c21270 at 2200.978 kb on - strand, within PGA1_c21270 at 2201.040 kb on + strand, within PGA1_c21270 at 2201.048 kb on - strand, within PGA1_c21270 at 2201.061 kb on + strand, within PGA1_c21270 at 2201.069 kb on - strand, within PGA1_c21270 at 2201.155 kb on - strand, within PGA1_c21270 at 2201.162 kb on + strand, within PGA1_c21270 at 2201.170 kb on - strand, within PGA1_c21270 at 2201.185 kb on + strand, within PGA1_c21270 at 2201.219 kb on + strand, within PGA1_c21270 at 2201.225 kb on - strand, within PGA1_c21270 at 2201.227 kb on - strand, within PGA1_c21270 at 2201.232 kb on + strand, within PGA1_c21270 at 2201.331 kb on - strand at 2201.461 kb on + strand at 2201.471 kb on + strand at 2201.471 kb on + strand at 2201.479 kb on - strand at 2201.479 kb on - strand at 2201.497 kb on - strand at 2201.523 kb on - strand at 2201.543 kb on - strand at 2201.550 kb on - strand at 2201.560 kb on - strand at 2201.563 kb on - strand at 2201.570 kb on + strand at 2201.676 kb on - strand, within PGA1_c21280 at 2201.708 kb on + strand, within PGA1_c21280 at 2201.726 kb on - strand, within PGA1_c21280 at 2201.749 kb on + strand, within PGA1_c21280 at 2201.772 kb on + strand, within PGA1_c21280 at 2201.781 kb on - strand, within PGA1_c21280 at 2201.782 kb on + strand, within PGA1_c21280 at 2201.807 kb on + strand, within PGA1_c21280 at 2201.829 kb on + strand, within PGA1_c21280 at 2201.855 kb on + strand, within PGA1_c21280 at 2201.863 kb on - strand, within PGA1_c21280 at 2201.863 kb on - strand, within PGA1_c21280 at 2201.934 kb on + strand, within PGA1_c21280 at 2201.934 kb on + strand, within PGA1_c21280 at 2201.942 kb on + strand, within PGA1_c21280 at 2201.942 kb on - strand, within PGA1_c21280 at 2201.948 kb on - strand, within PGA1_c21280 at 2202.032 kb on - strand, within PGA1_c21280 at 2202.117 kb on - strand, within PGA1_c21280 at 2202.190 kb on - strand, within PGA1_c21280 at 2202.208 kb on + strand, within PGA1_c21280 at 2202.244 kb on - strand, within PGA1_c21280 at 2202.310 kb on + strand, within PGA1_c21280 at 2202.337 kb on + strand, within PGA1_c21280 at 2202.340 kb on - strand, within PGA1_c21280 at 2202.342 kb on + strand, within PGA1_c21280 at 2202.345 kb on - strand, within PGA1_c21280 at 2202.363 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cisplatin 0.025 mg/ml remove 2,199,517 - PGA1_c21250 0.76 -0.1 2,199,522 - PGA1_c21250 0.76 +1.3 2,199,548 - PGA1_c21250 0.78 -1.7 2,199,621 + PGA1_c21250 0.84 -1.6 2,199,807 - +0.5 2,199,807 - +0.3 2,199,809 + -1.5 2,199,817 - -0.7 2,199,817 - -1.5 2,199,817 - -0.4 2,199,817 - +0.5 2,199,842 - -0.7 2,199,845 - +0.2 2,199,996 - PGA1_c21260 0.40 -0.4 2,200,052 + PGA1_c21260 0.54 +0.9 2,200,084 + PGA1_c21260 0.61 +1.5 2,200,107 + PGA1_c21260 0.67 +1.6 2,200,120 - PGA1_c21260 0.70 +1.1 2,200,168 + PGA1_c21260 0.81 +0.9 2,200,175 + PGA1_c21260 0.83 -0.3 2,200,185 + PGA1_c21260 0.85 +1.4 2,200,241 + -3.0 2,200,241 + -0.1 2,200,309 + +0.2 2,200,309 + +1.3 2,200,317 - -0.1 2,200,317 - +0.1 2,200,328 - -0.6 2,200,340 + -1.1 2,200,377 - +0.9 2,200,436 - +1.7 2,200,436 - -0.2 2,200,445 + +0.2 2,200,445 + -1.9 2,200,445 + +0.2 2,200,445 + +0.5 2,200,453 - +0.6 2,200,453 - -0.2 2,200,453 - -2.7 2,200,468 + -0.5 2,200,470 + +0.9 2,200,478 - +0.4 2,200,497 - +0.9 2,200,534 + +0.1 2,200,534 + -0.2 2,200,534 + +0.5 2,200,539 + -1.1 2,200,542 - -1.2 2,200,626 - PGA1_c21270 0.15 +0.5 2,200,737 + PGA1_c21270 0.28 -0.1 2,200,748 - PGA1_c21270 0.29 -0.1 2,200,813 + PGA1_c21270 0.36 -1.4 2,200,818 + PGA1_c21270 0.37 -2.3 2,200,819 + PGA1_c21270 0.37 -0.1 2,200,821 - PGA1_c21270 0.37 -2.5 2,200,821 - PGA1_c21270 0.37 -0.9 2,200,978 - PGA1_c21270 0.55 -0.5 2,201,040 + PGA1_c21270 0.63 +0.2 2,201,048 - PGA1_c21270 0.63 -3.7 2,201,061 + PGA1_c21270 0.65 +1.4 2,201,069 - PGA1_c21270 0.66 -1.1 2,201,155 - PGA1_c21270 0.76 +0.6 2,201,162 + PGA1_c21270 0.77 -0.1 2,201,170 - PGA1_c21270 0.78 -1.1 2,201,185 + PGA1_c21270 0.79 -1.6 2,201,219 + PGA1_c21270 0.83 +0.1 2,201,225 - PGA1_c21270 0.84 -0.3 2,201,227 - PGA1_c21270 0.84 +0.5 2,201,232 + PGA1_c21270 0.85 -0.2 2,201,331 - -0.3 2,201,461 + -1.1 2,201,471 + -1.1 2,201,471 + +0.0 2,201,479 - +0.9 2,201,479 - -0.1 2,201,497 - +0.1 2,201,523 - -1.7 2,201,543 - -0.7 2,201,550 - -0.1 2,201,560 - -1.1 2,201,563 - -1.5 2,201,570 + -1.5 2,201,676 - PGA1_c21280 0.15 +1.0 2,201,708 + PGA1_c21280 0.19 -1.1 2,201,726 - PGA1_c21280 0.21 +1.9 2,201,749 + PGA1_c21280 0.23 +2.8 2,201,772 + PGA1_c21280 0.26 +1.0 2,201,781 - PGA1_c21280 0.27 -0.6 2,201,782 + PGA1_c21280 0.27 +0.1 2,201,807 + PGA1_c21280 0.30 -0.9 2,201,829 + PGA1_c21280 0.32 +0.3 2,201,855 + PGA1_c21280 0.35 +0.6 2,201,863 - PGA1_c21280 0.36 -1.1 2,201,863 - PGA1_c21280 0.36 -0.6 2,201,934 + PGA1_c21280 0.43 +0.4 2,201,934 + PGA1_c21280 0.43 +0.7 2,201,942 + PGA1_c21280 0.44 -0.4 2,201,942 - PGA1_c21280 0.44 -0.4 2,201,948 - PGA1_c21280 0.45 -0.5 2,202,032 - PGA1_c21280 0.54 -0.5 2,202,117 - PGA1_c21280 0.63 +1.5 2,202,190 - PGA1_c21280 0.71 -0.0 2,202,208 + PGA1_c21280 0.73 -0.4 2,202,244 - PGA1_c21280 0.77 +0.0 2,202,310 + PGA1_c21280 0.84 -0.0 2,202,337 + PGA1_c21280 0.87 +0.7 2,202,340 - PGA1_c21280 0.88 -1.1 2,202,342 + PGA1_c21280 0.88 +0.5 2,202,345 - PGA1_c21280 0.88 +0.6 2,202,363 + -2.7
Or see this region's nucleotide sequence