Experiment: m.b. Cisplatin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c02010 and PGA1_c02020 are separated by 148 nucleotides PGA1_c02020 and PGA1_c02030 are separated by 218 nucleotides PGA1_c02030 and PGA1_c02040 overlap by 4 nucleotides PGA1_c02040 and PGA1_c02050 are separated by 199 nucleotides
PGA1_c02010: PGA1_c02010 - glutamate/glutamine/aspartate/asparagine transport ATP-binding protein BztD, at 184,295 to 185,086
_c02010
PGA1_c02020: PGA1_c02020 - Predicted metal-binding protein, at 185,235 to 185,693
_c02020
PGA1_c02030: PGA1_c02030 - phosphoglycerate mutase-like protein, at 185,912 to 186,409
_c02030
PGA1_c02040: PGA1_c02040 - hypothetical protein, at 186,406 to 187,149
_c02040
PGA1_c02050: PGA1_c02050 - acetylglutamate kinase ArgB, at 187,349 to 188,209
_c02050
Position (kb)
185
186
187 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 184.912 kb on + strand, within PGA1_c02010 at 184.919 kb on + strand, within PGA1_c02010 at 184.929 kb on + strand, within PGA1_c02010 at 185.082 kb on + strand at 185.090 kb on - strand at 185.215 kb on + strand at 185.253 kb on + strand at 185.285 kb on - strand, within PGA1_c02020 at 185.323 kb on - strand, within PGA1_c02020 at 185.438 kb on + strand, within PGA1_c02020 at 185.438 kb on + strand, within PGA1_c02020 at 185.438 kb on - strand, within PGA1_c02020 at 185.446 kb on - strand, within PGA1_c02020 at 185.537 kb on + strand, within PGA1_c02020 at 185.545 kb on - strand, within PGA1_c02020 at 185.553 kb on - strand, within PGA1_c02020 at 185.688 kb on + strand at 185.717 kb on + strand at 185.717 kb on + strand at 185.725 kb on - strand at 185.736 kb on - strand at 185.911 kb on - strand at 185.913 kb on - strand at 186.010 kb on + strand, within PGA1_c02030 at 186.124 kb on + strand, within PGA1_c02030 at 186.246 kb on - strand, within PGA1_c02030 at 186.353 kb on + strand, within PGA1_c02030 at 186.391 kb on + strand at 186.457 kb on + strand at 186.527 kb on + strand, within PGA1_c02040 at 186.535 kb on - strand, within PGA1_c02040 at 186.610 kb on + strand, within PGA1_c02040 at 186.610 kb on + strand, within PGA1_c02040 at 186.610 kb on + strand, within PGA1_c02040 at 186.618 kb on - strand, within PGA1_c02040 at 186.618 kb on - strand, within PGA1_c02040 at 186.618 kb on - strand, within PGA1_c02040 at 186.625 kb on + strand, within PGA1_c02040 at 186.651 kb on - strand, within PGA1_c02040 at 186.651 kb on - strand, within PGA1_c02040 at 186.656 kb on - strand, within PGA1_c02040 at 186.769 kb on - strand, within PGA1_c02040 at 186.779 kb on - strand, within PGA1_c02040 at 186.871 kb on + strand, within PGA1_c02040 at 186.903 kb on + strand, within PGA1_c02040 at 186.903 kb on + strand, within PGA1_c02040 at 186.997 kb on - strand, within PGA1_c02040 at 186.999 kb on + strand, within PGA1_c02040 at 187.005 kb on + strand, within PGA1_c02040 at 187.007 kb on + strand, within PGA1_c02040 at 187.013 kb on - strand, within PGA1_c02040 at 187.144 kb on + strand at 187.193 kb on + strand at 187.193 kb on + strand at 187.193 kb on + strand at 187.193 kb on - strand at 187.225 kb on + strand at 187.244 kb on - strand at 187.347 kb on + strand at 187.355 kb on - strand at 187.355 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cisplatin 0.025 mg/ml remove 184,912 + PGA1_c02010 0.78 +0.4 184,919 + PGA1_c02010 0.79 +0.6 184,929 + PGA1_c02010 0.80 +0.6 185,082 + +0.8 185,090 - +0.7 185,215 + -1.9 185,253 + +0.3 185,285 - PGA1_c02020 0.11 -0.7 185,323 - PGA1_c02020 0.19 +0.2 185,438 + PGA1_c02020 0.44 -0.7 185,438 + PGA1_c02020 0.44 +0.6 185,438 - PGA1_c02020 0.44 -1.2 185,446 - PGA1_c02020 0.46 +0.5 185,537 + PGA1_c02020 0.66 +0.1 185,545 - PGA1_c02020 0.68 +1.1 185,553 - PGA1_c02020 0.69 -2.9 185,688 + -0.0 185,717 + +0.4 185,717 + -2.6 185,725 - -0.1 185,736 - -0.9 185,911 - +0.2 185,913 - -1.7 186,010 + PGA1_c02030 0.20 +3.0 186,124 + PGA1_c02030 0.43 -2.3 186,246 - PGA1_c02030 0.67 +0.5 186,353 + PGA1_c02030 0.89 +0.4 186,391 + -1.1 186,457 + -0.4 186,527 + PGA1_c02040 0.16 +0.7 186,535 - PGA1_c02040 0.17 -0.3 186,610 + PGA1_c02040 0.27 +0.6 186,610 + PGA1_c02040 0.27 +0.3 186,610 + PGA1_c02040 0.27 -1.0 186,618 - PGA1_c02040 0.28 -1.5 186,618 - PGA1_c02040 0.28 +0.3 186,618 - PGA1_c02040 0.28 -0.3 186,625 + PGA1_c02040 0.29 +0.6 186,651 - PGA1_c02040 0.33 -1.5 186,651 - PGA1_c02040 0.33 +0.3 186,656 - PGA1_c02040 0.34 -1.2 186,769 - PGA1_c02040 0.49 -0.3 186,779 - PGA1_c02040 0.50 +0.4 186,871 + PGA1_c02040 0.62 -1.0 186,903 + PGA1_c02040 0.67 -2.2 186,903 + PGA1_c02040 0.67 -1.0 186,997 - PGA1_c02040 0.79 +2.4 186,999 + PGA1_c02040 0.80 -0.5 187,005 + PGA1_c02040 0.81 +0.6 187,007 + PGA1_c02040 0.81 +0.7 187,013 - PGA1_c02040 0.82 -0.4 187,144 + -0.1 187,193 + +2.2 187,193 + +0.4 187,193 + -0.5 187,193 - -0.6 187,225 + -1.6 187,244 - -2.0 187,347 + -0.9 187,355 - -0.4 187,355 - +0.6
Or see this region's nucleotide sequence