Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02615 and BWI76_RS02620 are separated by 364 nucleotides BWI76_RS02620 and BWI76_RS02625 are separated by 12 nucleotides BWI76_RS02625 and BWI76_RS02630 are separated by 9 nucleotides BWI76_RS02630 and BWI76_RS02635 are separated by 13 nucleotides
BWI76_RS02615: BWI76_RS02615 - L-ascorbate-6-phosphate lactonase, at 534,628 to 535,692
_RS02615
BWI76_RS02620: BWI76_RS02620 - PTS ascorbate transporter subunit IIC, at 536,057 to 537,457
_RS02620
BWI76_RS02625: BWI76_RS02625 - PTS ascorbate transporter subunit IIB, at 537,470 to 537,775
_RS02625
BWI76_RS02630: BWI76_RS02630 - PTS ascorbate transporter subunit IIA, at 537,785 to 538,252
_RS02630
BWI76_RS02635: BWI76_RS02635 - 3-keto-L-gulonate-6-phosphate decarboxylase, at 538,266 to 538,916
_RS02635
Position (kb) 536
537
538 Strain fitness (log2 ratio) -1
0
1
2
3 at 535.131 kb on - strand, within BWI76_RS02615 at 535.134 kb on - strand, within BWI76_RS02615 at 535.134 kb on - strand, within BWI76_RS02615 at 535.156 kb on - strand, within BWI76_RS02615 at 535.240 kb on + strand, within BWI76_RS02615 at 535.241 kb on - strand, within BWI76_RS02615 at 535.291 kb on + strand, within BWI76_RS02615 at 535.299 kb on - strand at 535.415 kb on + strand, within BWI76_RS02615 at 535.527 kb on - strand, within BWI76_RS02615 at 535.602 kb on - strand at 535.799 kb on + strand at 536.082 kb on - strand at 536.098 kb on + strand at 536.106 kb on - strand at 536.145 kb on + strand at 536.153 kb on - strand at 536.216 kb on - strand, within BWI76_RS02620 at 536.216 kb on - strand, within BWI76_RS02620 at 536.245 kb on - strand, within BWI76_RS02620 at 536.275 kb on + strand, within BWI76_RS02620 at 536.283 kb on - strand, within BWI76_RS02620 at 536.344 kb on + strand, within BWI76_RS02620 at 536.354 kb on + strand, within BWI76_RS02620 at 536.368 kb on + strand at 536.370 kb on + strand, within BWI76_RS02620 at 536.376 kb on - strand, within BWI76_RS02620 at 536.389 kb on - strand, within BWI76_RS02620 at 536.406 kb on - strand, within BWI76_RS02620 at 536.533 kb on + strand, within BWI76_RS02620 at 536.568 kb on - strand, within BWI76_RS02620 at 536.712 kb on + strand, within BWI76_RS02620 at 536.827 kb on + strand, within BWI76_RS02620 at 536.836 kb on + strand, within BWI76_RS02620 at 536.844 kb on - strand, within BWI76_RS02620 at 536.856 kb on + strand, within BWI76_RS02620 at 536.905 kb on + strand, within BWI76_RS02620 at 537.035 kb on + strand, within BWI76_RS02620 at 537.115 kb on - strand, within BWI76_RS02620 at 537.241 kb on + strand, within BWI76_RS02620 at 537.331 kb on - strand at 537.376 kb on + strand at 537.397 kb on + strand at 537.487 kb on + strand at 537.491 kb on + strand at 537.529 kb on + strand, within BWI76_RS02625 at 537.583 kb on - strand, within BWI76_RS02625 at 537.590 kb on - strand, within BWI76_RS02625 at 537.644 kb on - strand, within BWI76_RS02625 at 537.824 kb on - strand at 537.868 kb on + strand, within BWI76_RS02630 at 537.908 kb on + strand, within BWI76_RS02630 at 537.941 kb on - strand, within BWI76_RS02630 at 538.020 kb on + strand, within BWI76_RS02630 at 538.154 kb on - strand, within BWI76_RS02630 at 538.156 kb on + strand, within BWI76_RS02630 at 538.168 kb on + strand, within BWI76_RS02630 at 538.334 kb on + strand, within BWI76_RS02635 at 538.342 kb on - strand, within BWI76_RS02635
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 535,131 - BWI76_RS02615 0.47 -0.4 535,134 - BWI76_RS02615 0.48 -0.4 535,134 - BWI76_RS02615 0.48 -0.7 535,156 - BWI76_RS02615 0.50 +0.0 535,240 + BWI76_RS02615 0.57 +1.1 535,241 - BWI76_RS02615 0.58 -0.8 535,291 + BWI76_RS02615 0.62 -0.3 535,299 - +2.2 535,415 + BWI76_RS02615 0.74 +0.2 535,527 - BWI76_RS02615 0.84 -0.3 535,602 - -0.7 535,799 + -0.2 536,082 - -0.8 536,098 + +0.4 536,106 - -0.2 536,145 + +1.2 536,153 - +0.2 536,216 - BWI76_RS02620 0.11 +0.2 536,216 - BWI76_RS02620 0.11 -0.4 536,245 - BWI76_RS02620 0.13 +0.8 536,275 + BWI76_RS02620 0.16 +0.2 536,283 - BWI76_RS02620 0.16 -0.1 536,344 + BWI76_RS02620 0.20 -0.4 536,354 + BWI76_RS02620 0.21 +0.7 536,368 + +1.2 536,370 + BWI76_RS02620 0.22 +0.2 536,376 - BWI76_RS02620 0.23 -1.5 536,389 - BWI76_RS02620 0.24 +0.9 536,406 - BWI76_RS02620 0.25 +0.4 536,533 + BWI76_RS02620 0.34 -0.1 536,568 - BWI76_RS02620 0.36 +1.2 536,712 + BWI76_RS02620 0.47 -0.7 536,827 + BWI76_RS02620 0.55 +0.4 536,836 + BWI76_RS02620 0.56 -1.3 536,844 - BWI76_RS02620 0.56 +0.4 536,856 + BWI76_RS02620 0.57 +0.5 536,905 + BWI76_RS02620 0.61 +0.1 537,035 + BWI76_RS02620 0.70 -0.0 537,115 - BWI76_RS02620 0.76 -0.4 537,241 + BWI76_RS02620 0.85 +0.6 537,331 - -1.1 537,376 + -0.6 537,397 + +0.6 537,487 + +0.3 537,491 + +0.0 537,529 + BWI76_RS02625 0.19 +0.4 537,583 - BWI76_RS02625 0.37 -0.2 537,590 - BWI76_RS02625 0.39 +0.6 537,644 - BWI76_RS02625 0.57 -0.1 537,824 - +2.8 537,868 + BWI76_RS02630 0.18 -0.2 537,908 + BWI76_RS02630 0.26 +0.6 537,941 - BWI76_RS02630 0.33 -0.1 538,020 + BWI76_RS02630 0.50 +1.9 538,154 - BWI76_RS02630 0.79 +0.1 538,156 + BWI76_RS02630 0.79 -0.8 538,168 + BWI76_RS02630 0.82 -0.4 538,334 + BWI76_RS02635 0.10 -0.6 538,342 - BWI76_RS02635 0.12 -0.0
Or see this region's nucleotide sequence