Experiment: reconstituted MRS with Maltodextrin 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15675 and BBR_RS15680 overlap by 1 nucleotides BBR_RS15680 and BBR_RS15685 are separated by 19 nucleotides BBR_RS15685 and BBR_RS15690 overlap by 53 nucleotides
BBR_RS15675: BBR_RS15675 - ADP-forming succinate--CoA ligase subunit beta, at 1,285,416 to 1,286,618
_RS15675
BBR_RS15680: BBR_RS15680 - succinate--CoA ligase subunit alpha, at 1,286,618 to 1,287,529
_RS15680
BBR_RS15685: BBR_RS15685 - hypothetical protein, at 1,287,549 to 1,288,934
_RS15685
BBR_RS15690: BBR_RS15690 - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase PurH, at 1,288,882 to 1,290,519
_RS15690
Position (kb)
1287
1288
1289 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1286.584 kb on + strand at 1286.597 kb on - strand at 1286.600 kb on + strand at 1286.600 kb on + strand at 1286.600 kb on + strand at 1286.632 kb on + strand at 1286.656 kb on + strand at 1286.656 kb on + strand at 1286.656 kb on + strand at 1286.656 kb on + strand at 1286.664 kb on - strand at 1286.664 kb on - strand at 1286.688 kb on - strand at 1286.688 kb on - strand at 1286.701 kb on - strand at 1286.735 kb on - strand, within BBR_RS15680 at 1286.750 kb on - strand, within BBR_RS15680 at 1286.858 kb on - strand, within BBR_RS15680 at 1286.973 kb on + strand, within BBR_RS15680 at 1286.983 kb on + strand, within BBR_RS15680 at 1286.983 kb on + strand, within BBR_RS15680 at 1286.983 kb on + strand, within BBR_RS15680 at 1286.983 kb on + strand, within BBR_RS15680 at 1286.983 kb on + strand, within BBR_RS15680 at 1286.990 kb on + strand, within BBR_RS15680 at 1287.003 kb on - strand, within BBR_RS15680 at 1287.023 kb on + strand, within BBR_RS15680 at 1287.095 kb on + strand, within BBR_RS15680 at 1287.124 kb on - strand, within BBR_RS15680 at 1287.177 kb on - strand, within BBR_RS15680 at 1287.177 kb on - strand, within BBR_RS15680 at 1287.198 kb on + strand, within BBR_RS15680 at 1287.198 kb on + strand, within BBR_RS15680 at 1287.198 kb on + strand, within BBR_RS15680 at 1287.198 kb on + strand, within BBR_RS15680 at 1287.206 kb on - strand, within BBR_RS15680 at 1287.206 kb on - strand, within BBR_RS15680 at 1287.307 kb on + strand, within BBR_RS15680 at 1287.461 kb on - strand at 1287.464 kb on + strand at 1287.471 kb on - strand at 1287.483 kb on - strand at 1287.498 kb on - strand at 1287.621 kb on + strand at 1287.621 kb on + strand at 1287.629 kb on - strand at 1287.661 kb on + strand at 1287.661 kb on + strand at 1287.697 kb on + strand, within BBR_RS15685 at 1287.723 kb on - strand, within BBR_RS15685 at 1287.757 kb on + strand, within BBR_RS15685 at 1287.757 kb on + strand, within BBR_RS15685 at 1287.757 kb on + strand, within BBR_RS15685 at 1287.765 kb on - strand, within BBR_RS15685 at 1287.769 kb on + strand, within BBR_RS15685 at 1287.769 kb on + strand, within BBR_RS15685 at 1287.790 kb on - strand, within BBR_RS15685 at 1287.800 kb on - strand, within BBR_RS15685 at 1287.805 kb on - strand, within BBR_RS15685 at 1287.830 kb on + strand, within BBR_RS15685 at 1287.866 kb on - strand, within BBR_RS15685 at 1287.866 kb on - strand, within BBR_RS15685 at 1287.922 kb on - strand, within BBR_RS15685 at 1287.927 kb on - strand, within BBR_RS15685 at 1287.953 kb on + strand, within BBR_RS15685 at 1287.961 kb on - strand, within BBR_RS15685 at 1287.997 kb on + strand, within BBR_RS15685 at 1288.005 kb on + strand, within BBR_RS15685 at 1288.007 kb on + strand, within BBR_RS15685 at 1288.007 kb on + strand, within BBR_RS15685 at 1288.015 kb on - strand, within BBR_RS15685 at 1288.015 kb on - strand, within BBR_RS15685 at 1288.024 kb on - strand, within BBR_RS15685 at 1288.069 kb on - strand, within BBR_RS15685 at 1288.111 kb on + strand, within BBR_RS15685 at 1288.119 kb on - strand, within BBR_RS15685 at 1288.174 kb on - strand, within BBR_RS15685 at 1288.236 kb on - strand, within BBR_RS15685 at 1288.281 kb on - strand, within BBR_RS15685 at 1288.288 kb on + strand, within BBR_RS15685 at 1288.301 kb on - strand, within BBR_RS15685 at 1288.326 kb on - strand, within BBR_RS15685 at 1288.330 kb on + strand, within BBR_RS15685 at 1288.330 kb on + strand, within BBR_RS15685 at 1288.335 kb on + strand, within BBR_RS15685 at 1288.335 kb on + strand, within BBR_RS15685 at 1288.338 kb on - strand, within BBR_RS15685 at 1288.340 kb on + strand, within BBR_RS15685 at 1288.340 kb on + strand, within BBR_RS15685 at 1288.348 kb on - strand, within BBR_RS15685 at 1288.348 kb on - strand, within BBR_RS15685 at 1288.348 kb on - strand, within BBR_RS15685 at 1288.348 kb on - strand, within BBR_RS15685 at 1288.348 kb on - strand, within BBR_RS15685 at 1288.348 kb on - strand, within BBR_RS15685 at 1288.348 kb on - strand, within BBR_RS15685 at 1288.367 kb on - strand, within BBR_RS15685 at 1288.387 kb on - strand, within BBR_RS15685 at 1288.387 kb on - strand, within BBR_RS15685 at 1288.393 kb on - strand, within BBR_RS15685 at 1288.401 kb on + strand, within BBR_RS15685 at 1288.421 kb on - strand, within BBR_RS15685 at 1288.470 kb on - strand, within BBR_RS15685 at 1288.470 kb on - strand, within BBR_RS15685 at 1288.494 kb on + strand, within BBR_RS15685 at 1288.507 kb on - strand, within BBR_RS15685 at 1288.507 kb on - strand, within BBR_RS15685 at 1288.539 kb on + strand, within BBR_RS15685 at 1288.612 kb on - strand, within BBR_RS15685 at 1288.657 kb on + strand, within BBR_RS15685 at 1288.700 kb on + strand, within BBR_RS15685 at 1288.708 kb on - strand, within BBR_RS15685 at 1288.823 kb on + strand at 1288.842 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Maltodextrin 0.5 %w/v remove 1,286,584 + +0.4 1,286,597 - +0.9 1,286,600 + +0.5 1,286,600 + +0.0 1,286,600 + -0.4 1,286,632 + +0.0 1,286,656 + -3.3 1,286,656 + -0.4 1,286,656 + -3.3 1,286,656 + -2.7 1,286,664 - +0.5 1,286,664 - -1.3 1,286,688 - -2.7 1,286,688 - -1.1 1,286,701 - -2.1 1,286,735 - BBR_RS15680 0.13 -0.3 1,286,750 - BBR_RS15680 0.14 -0.6 1,286,858 - BBR_RS15680 0.26 -1.8 1,286,973 + BBR_RS15680 0.39 -1.8 1,286,983 + BBR_RS15680 0.40 -0.8 1,286,983 + BBR_RS15680 0.40 -0.8 1,286,983 + BBR_RS15680 0.40 -2.6 1,286,983 + BBR_RS15680 0.40 -1.0 1,286,983 + BBR_RS15680 0.40 +0.1 1,286,990 + BBR_RS15680 0.41 -1.9 1,287,003 - BBR_RS15680 0.42 -0.3 1,287,023 + BBR_RS15680 0.44 -2.7 1,287,095 + BBR_RS15680 0.52 -1.8 1,287,124 - BBR_RS15680 0.55 -2.4 1,287,177 - BBR_RS15680 0.61 -0.7 1,287,177 - BBR_RS15680 0.61 -2.2 1,287,198 + BBR_RS15680 0.64 -2.0 1,287,198 + BBR_RS15680 0.64 -0.9 1,287,198 + BBR_RS15680 0.64 +0.8 1,287,198 + BBR_RS15680 0.64 -0.1 1,287,206 - BBR_RS15680 0.64 -2.7 1,287,206 - BBR_RS15680 0.64 -2.8 1,287,307 + BBR_RS15680 0.76 -1.2 1,287,461 - -0.8 1,287,464 + -1.7 1,287,471 - -2.1 1,287,483 - -3.7 1,287,498 - +0.6 1,287,621 + -3.2 1,287,621 + -2.2 1,287,629 - +0.8 1,287,661 + -1.4 1,287,661 + -3.5 1,287,697 + BBR_RS15685 0.11 -3.1 1,287,723 - BBR_RS15685 0.13 -0.6 1,287,757 + BBR_RS15685 0.15 -1.3 1,287,757 + BBR_RS15685 0.15 -0.9 1,287,757 + BBR_RS15685 0.15 -3.1 1,287,765 - BBR_RS15685 0.16 -1.1 1,287,769 + BBR_RS15685 0.16 -2.8 1,287,769 + BBR_RS15685 0.16 -1.7 1,287,790 - BBR_RS15685 0.17 -0.5 1,287,800 - BBR_RS15685 0.18 -2.9 1,287,805 - BBR_RS15685 0.18 -1.1 1,287,830 + BBR_RS15685 0.20 -3.7 1,287,866 - BBR_RS15685 0.23 -2.4 1,287,866 - BBR_RS15685 0.23 -2.8 1,287,922 - BBR_RS15685 0.27 -3.2 1,287,927 - BBR_RS15685 0.27 -1.8 1,287,953 + BBR_RS15685 0.29 -3.8 1,287,961 - BBR_RS15685 0.30 -0.9 1,287,997 + BBR_RS15685 0.32 -2.9 1,288,005 + BBR_RS15685 0.33 -0.2 1,288,007 + BBR_RS15685 0.33 -2.5 1,288,007 + BBR_RS15685 0.33 -1.9 1,288,015 - BBR_RS15685 0.34 -0.9 1,288,015 - BBR_RS15685 0.34 -2.0 1,288,024 - BBR_RS15685 0.34 -2.3 1,288,069 - BBR_RS15685 0.38 -0.8 1,288,111 + BBR_RS15685 0.41 -1.6 1,288,119 - BBR_RS15685 0.41 -3.4 1,288,174 - BBR_RS15685 0.45 -2.6 1,288,236 - BBR_RS15685 0.50 -1.1 1,288,281 - BBR_RS15685 0.53 -1.4 1,288,288 + BBR_RS15685 0.53 -1.6 1,288,301 - BBR_RS15685 0.54 -2.1 1,288,326 - BBR_RS15685 0.56 -1.9 1,288,330 + BBR_RS15685 0.56 -0.8 1,288,330 + BBR_RS15685 0.56 -1.7 1,288,335 + BBR_RS15685 0.57 -1.8 1,288,335 + BBR_RS15685 0.57 -3.2 1,288,338 - BBR_RS15685 0.57 -2.6 1,288,340 + BBR_RS15685 0.57 -0.5 1,288,340 + BBR_RS15685 0.57 -3.2 1,288,348 - BBR_RS15685 0.58 -2.1 1,288,348 - BBR_RS15685 0.58 -2.0 1,288,348 - BBR_RS15685 0.58 -2.6 1,288,348 - BBR_RS15685 0.58 -3.1 1,288,348 - BBR_RS15685 0.58 -1.3 1,288,348 - BBR_RS15685 0.58 -1.4 1,288,348 - BBR_RS15685 0.58 -0.7 1,288,367 - BBR_RS15685 0.59 -2.5 1,288,387 - BBR_RS15685 0.60 -2.7 1,288,387 - BBR_RS15685 0.60 -0.6 1,288,393 - BBR_RS15685 0.61 -2.3 1,288,401 + BBR_RS15685 0.61 -1.9 1,288,421 - BBR_RS15685 0.63 -2.6 1,288,470 - BBR_RS15685 0.66 -1.6 1,288,470 - BBR_RS15685 0.66 -2.7 1,288,494 + BBR_RS15685 0.68 -2.0 1,288,507 - BBR_RS15685 0.69 -2.1 1,288,507 - BBR_RS15685 0.69 -3.8 1,288,539 + BBR_RS15685 0.71 -1.6 1,288,612 - BBR_RS15685 0.77 -3.2 1,288,657 + BBR_RS15685 0.80 -1.9 1,288,700 + BBR_RS15685 0.83 -0.9 1,288,708 - BBR_RS15685 0.84 -2.3 1,288,823 + -1.6 1,288,842 - -0.8
Or see this region's nucleotide sequence