Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2503 and BT2504 are separated by 32 nucleotides BT2504 and BT2505 are separated by 113 nucleotides BT2505 and BT2506 are separated by 855 nucleotides
BT2503: BT2503 - hypothetical protein (NCBI ptt file), at 3,127,725 to 3,128,741
BT2503
BT2504: BT2504 - hypothetical protein (NCBI ptt file), at 3,128,774 to 3,129,454
BT2504
BT2505: BT2505 - conserved hypothetical protein (NCBI ptt file), at 3,129,568 to 3,130,407
BT2505
BT2506: BT2506 - hypothetical protein (NCBI ptt file), at 3,131,263 to 3,131,781
BT2506
Position (kb)
3129
3130
3131 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3128.670 kb on + strand at 3128.698 kb on - strand at 3128.724 kb on + strand at 3128.725 kb on - strand at 3128.726 kb on + strand at 3128.726 kb on + strand at 3128.726 kb on + strand at 3128.727 kb on - strand at 3128.730 kb on + strand at 3128.769 kb on + strand at 3128.834 kb on - strand at 3128.870 kb on + strand, within BT2504 at 3128.875 kb on + strand, within BT2504 at 3128.875 kb on + strand, within BT2504 at 3128.914 kb on + strand, within BT2504 at 3128.924 kb on - strand, within BT2504 at 3128.987 kb on - strand, within BT2504 at 3128.998 kb on + strand, within BT2504 at 3129.070 kb on + strand, within BT2504 at 3129.159 kb on - strand, within BT2504 at 3129.166 kb on + strand, within BT2504 at 3129.174 kb on - strand, within BT2504 at 3129.207 kb on + strand, within BT2504 at 3129.274 kb on + strand, within BT2504 at 3129.276 kb on + strand, within BT2504 at 3129.310 kb on + strand, within BT2504 at 3129.310 kb on + strand, within BT2504 at 3129.314 kb on - strand, within BT2504 at 3129.321 kb on + strand, within BT2504 at 3129.345 kb on + strand, within BT2504 at 3129.361 kb on + strand, within BT2504 at 3129.443 kb on + strand at 3129.454 kb on + strand at 3129.455 kb on - strand at 3129.455 kb on - strand at 3129.455 kb on - strand at 3129.459 kb on + strand at 3129.460 kb on - strand at 3129.539 kb on - strand at 3129.569 kb on + strand at 3129.623 kb on + strand at 3129.793 kb on + strand, within BT2505 at 3129.807 kb on + strand, within BT2505 at 3129.821 kb on - strand, within BT2505 at 3129.899 kb on + strand, within BT2505 at 3129.899 kb on + strand, within BT2505 at 3129.899 kb on + strand, within BT2505 at 3129.900 kb on - strand, within BT2505 at 3129.903 kb on + strand at 3129.903 kb on + strand, within BT2505 at 3129.904 kb on - strand, within BT2505 at 3129.904 kb on - strand, within BT2505 at 3129.904 kb on - strand, within BT2505 at 3129.954 kb on + strand, within BT2505 at 3129.977 kb on + strand, within BT2505 at 3130.117 kb on + strand, within BT2505 at 3130.117 kb on + strand, within BT2505 at 3130.117 kb on + strand, within BT2505 at 3130.258 kb on + strand, within BT2505 at 3130.276 kb on + strand, within BT2505 at 3130.282 kb on - strand, within BT2505 at 3130.295 kb on - strand, within BT2505 at 3130.366 kb on + strand at 3130.408 kb on - strand at 3130.421 kb on + strand at 3130.421 kb on + strand at 3130.429 kb on - strand at 3130.694 kb on + strand at 3130.709 kb on + strand at 3130.710 kb on - strand at 3130.721 kb on + strand at 3130.762 kb on - strand at 3130.787 kb on - strand at 3130.787 kb on - strand at 3130.799 kb on + strand at 3130.801 kb on + strand at 3130.806 kb on + strand at 3130.807 kb on - strand at 3130.856 kb on + strand at 3130.884 kb on - strand at 3130.884 kb on - strand at 3130.887 kb on + strand at 3130.895 kb on + strand at 3130.897 kb on + strand at 3130.898 kb on - strand at 3130.898 kb on - strand at 3130.898 kb on - strand at 3130.902 kb on + strand at 3130.918 kb on - strand at 3130.982 kb on + strand at 3130.982 kb on + strand at 3130.983 kb on - strand at 3130.984 kb on - strand at 3130.990 kb on - strand at 3131.011 kb on + strand at 3131.084 kb on + strand at 3131.116 kb on - strand at 3131.123 kb on - strand at 3131.196 kb on - strand at 3131.196 kb on - strand at 3131.254 kb on + strand at 3131.276 kb on + strand at 3131.307 kb on + strand at 3131.308 kb on - strand at 3131.308 kb on - strand at 3131.310 kb on - strand at 3131.333 kb on + strand, within BT2506 at 3131.333 kb on + strand, within BT2506 at 3131.334 kb on - strand, within BT2506 at 3131.340 kb on - strand, within BT2506 at 3131.345 kb on + strand, within BT2506 at 3131.346 kb on - strand, within BT2506 at 3131.382 kb on - strand, within BT2506
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 3,128,670 + +0.0 3,128,698 - -1.3 3,128,724 + -1.2 3,128,725 - -0.4 3,128,726 + -1.6 3,128,726 + -1.7 3,128,726 + -0.5 3,128,727 - -2.2 3,128,730 + +2.6 3,128,769 + +1.0 3,128,834 - -1.0 3,128,870 + BT2504 0.14 +0.0 3,128,875 + BT2504 0.15 -0.4 3,128,875 + BT2504 0.15 -0.5 3,128,914 + BT2504 0.21 -0.7 3,128,924 - BT2504 0.22 -1.0 3,128,987 - BT2504 0.31 -1.5 3,128,998 + BT2504 0.33 -1.1 3,129,070 + BT2504 0.43 -1.4 3,129,159 - BT2504 0.57 +0.0 3,129,166 + BT2504 0.58 +1.6 3,129,174 - BT2504 0.59 +1.0 3,129,207 + BT2504 0.64 -1.1 3,129,274 + BT2504 0.73 -0.5 3,129,276 + BT2504 0.74 -0.8 3,129,310 + BT2504 0.79 -1.0 3,129,310 + BT2504 0.79 +1.0 3,129,314 - BT2504 0.79 -3.1 3,129,321 + BT2504 0.80 +0.4 3,129,345 + BT2504 0.84 -2.3 3,129,361 + BT2504 0.86 -1.0 3,129,443 + +0.0 3,129,454 + +1.0 3,129,455 - -1.0 3,129,455 - -1.2 3,129,455 - -1.3 3,129,459 + +0.4 3,129,460 - +1.0 3,129,539 - -0.5 3,129,569 + -3.2 3,129,623 + -1.5 3,129,793 + BT2505 0.27 +1.3 3,129,807 + BT2505 0.28 -1.1 3,129,821 - BT2505 0.30 +1.0 3,129,899 + BT2505 0.39 -0.3 3,129,899 + BT2505 0.39 +0.0 3,129,899 + BT2505 0.39 -1.1 3,129,900 - BT2505 0.40 -1.7 3,129,903 + -0.5 3,129,903 + BT2505 0.40 -0.2 3,129,904 - BT2505 0.40 -0.5 3,129,904 - BT2505 0.40 +1.0 3,129,904 - BT2505 0.40 -1.0 3,129,954 + BT2505 0.46 +0.7 3,129,977 + BT2505 0.49 +1.6 3,130,117 + BT2505 0.65 -1.6 3,130,117 + BT2505 0.65 -1.7 3,130,117 + BT2505 0.65 -0.8 3,130,258 + BT2505 0.82 +1.0 3,130,276 + BT2505 0.84 -2.5 3,130,282 - BT2505 0.85 +0.0 3,130,295 - BT2505 0.87 -0.5 3,130,366 + -1.2 3,130,408 - -0.2 3,130,421 + +0.0 3,130,421 + -1.4 3,130,429 - -1.4 3,130,694 + +0.0 3,130,709 + +1.0 3,130,710 - -0.9 3,130,721 + -0.3 3,130,762 - -1.0 3,130,787 - +1.6 3,130,787 - -2.3 3,130,799 + -0.5 3,130,801 + +0.2 3,130,806 + -0.8 3,130,807 - -1.0 3,130,856 + -1.3 3,130,884 - -0.4 3,130,884 - +2.0 3,130,887 + +0.5 3,130,895 + -1.7 3,130,897 + +0.0 3,130,898 - -2.7 3,130,898 - -1.8 3,130,898 - -1.2 3,130,902 + +2.0 3,130,918 - +0.0 3,130,982 + -0.5 3,130,982 + +2.0 3,130,983 - -1.9 3,130,984 - -1.4 3,130,990 - +0.0 3,131,011 + +0.0 3,131,084 + -1.0 3,131,116 - +2.6 3,131,123 - -0.9 3,131,196 - -1.5 3,131,196 - +0.7 3,131,254 + -0.9 3,131,276 + +0.2 3,131,307 + -2.1 3,131,308 - -1.2 3,131,308 - +1.0 3,131,310 - -1.3 3,131,333 + BT2506 0.13 -1.2 3,131,333 + BT2506 0.13 -0.4 3,131,334 - BT2506 0.14 +0.5 3,131,340 - BT2506 0.15 +1.5 3,131,345 + BT2506 0.16 +0.3 3,131,346 - BT2506 0.16 +0.0 3,131,382 - BT2506 0.23 -1.4
Or see this region's nucleotide sequence