Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2099 and BT2100 overlap by 4 nucleotides BT2100 and BT2101 are separated by 123 nucleotides
BT2099: BT2099 - Fe3+ ABC transporter, permease (NCBI ptt file), at 2,633,396 to 2,634,334
BT2099
BT2100: BT2100 - putative ABC transporter, ATP-binding protein (NCBI ptt file), at 2,634,331 to 2,635,086
BT2100
BT2101: BT2101 - putative transmembrane cation efflux protein (NCBI ptt file), at 2,635,210 to 2,636,565
BT2101
Position (kb)
2634
2635
2636 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2633.383 kb on - strand at 2633.386 kb on - strand at 2633.391 kb on + strand at 2633.391 kb on + strand at 2633.391 kb on + strand at 2633.415 kb on - strand at 2633.415 kb on - strand at 2633.415 kb on - strand at 2633.415 kb on - strand at 2633.433 kb on + strand at 2633.512 kb on - strand, within BT2099 at 2633.512 kb on - strand, within BT2099 at 2633.512 kb on - strand, within BT2099 at 2633.512 kb on - strand, within BT2099 at 2633.527 kb on - strand, within BT2099 at 2633.641 kb on + strand, within BT2099 at 2633.643 kb on + strand, within BT2099 at 2633.646 kb on + strand, within BT2099 at 2633.661 kb on + strand, within BT2099 at 2633.773 kb on + strand, within BT2099 at 2633.811 kb on - strand, within BT2099 at 2633.949 kb on + strand, within BT2099 at 2634.068 kb on - strand, within BT2099 at 2634.177 kb on + strand, within BT2099 at 2634.209 kb on - strand, within BT2099 at 2634.307 kb on - strand at 2634.310 kb on + strand at 2634.372 kb on + strand at 2634.373 kb on - strand at 2634.519 kb on + strand, within BT2100 at 2634.637 kb on + strand, within BT2100 at 2634.637 kb on + strand, within BT2100 at 2634.719 kb on - strand, within BT2100 at 2634.901 kb on - strand, within BT2100 at 2634.966 kb on + strand, within BT2100 at 2635.019 kb on + strand at 2635.019 kb on + strand at 2635.020 kb on - strand at 2635.084 kb on + strand at 2635.135 kb on - strand at 2635.170 kb on - strand at 2635.219 kb on - strand at 2635.219 kb on - strand at 2635.221 kb on + strand at 2635.221 kb on + strand at 2635.222 kb on - strand at 2635.236 kb on + strand at 2635.236 kb on + strand at 2635.268 kb on + strand at 2635.304 kb on + strand at 2635.326 kb on - strand at 2635.332 kb on - strand at 2635.371 kb on + strand, within BT2101 at 2635.372 kb on - strand, within BT2101 at 2635.373 kb on + strand, within BT2101 at 2635.390 kb on - strand, within BT2101 at 2635.482 kb on + strand, within BT2101 at 2635.491 kb on + strand, within BT2101 at 2635.492 kb on - strand, within BT2101 at 2635.524 kb on - strand, within BT2101 at 2635.574 kb on + strand, within BT2101 at 2635.611 kb on - strand, within BT2101 at 2635.616 kb on - strand, within BT2101 at 2635.617 kb on + strand, within BT2101 at 2635.618 kb on - strand, within BT2101 at 2635.618 kb on - strand, within BT2101 at 2635.703 kb on - strand, within BT2101 at 2635.740 kb on + strand, within BT2101 at 2635.743 kb on + strand, within BT2101 at 2635.821 kb on - strand, within BT2101 at 2635.823 kb on + strand, within BT2101 at 2635.824 kb on - strand, within BT2101 at 2635.841 kb on + strand, within BT2101 at 2635.846 kb on + strand, within BT2101 at 2635.914 kb on - strand, within BT2101 at 2635.934 kb on - strand, within BT2101 at 2635.936 kb on - strand, within BT2101 at 2635.940 kb on + strand, within BT2101 at 2635.987 kb on - strand, within BT2101 at 2635.987 kb on - strand, within BT2101 at 2636.062 kb on + strand, within BT2101
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 2,633,383 - +0.0 2,633,386 - -1.3 2,633,391 + +3.0 2,633,391 + +2.0 2,633,391 + -1.0 2,633,415 - +0.2 2,633,415 - -0.2 2,633,415 - +0.3 2,633,415 - -1.4 2,633,433 + -0.3 2,633,512 - BT2099 0.12 +0.3 2,633,512 - BT2099 0.12 -0.3 2,633,512 - BT2099 0.12 +0.2 2,633,512 - BT2099 0.12 -1.0 2,633,527 - BT2099 0.14 +2.0 2,633,641 + BT2099 0.26 -2.3 2,633,643 + BT2099 0.26 +0.5 2,633,646 + BT2099 0.27 +0.3 2,633,661 + BT2099 0.28 -1.8 2,633,773 + BT2099 0.40 -2.4 2,633,811 - BT2099 0.44 -1.0 2,633,949 + BT2099 0.59 +1.6 2,634,068 - BT2099 0.72 -0.1 2,634,177 + BT2099 0.83 +0.0 2,634,209 - BT2099 0.87 -0.6 2,634,307 - -1.1 2,634,310 + +1.0 2,634,372 + -0.2 2,634,373 - -1.2 2,634,519 + BT2100 0.25 -0.9 2,634,637 + BT2100 0.40 -0.0 2,634,637 + BT2100 0.40 -0.5 2,634,719 - BT2100 0.51 +1.0 2,634,901 - BT2100 0.75 +1.6 2,634,966 + BT2100 0.84 +0.0 2,635,019 + -3.3 2,635,019 + -1.3 2,635,020 - +2.4 2,635,084 + +0.0 2,635,135 - -1.5 2,635,170 - -2.4 2,635,219 - -1.5 2,635,219 - +2.2 2,635,221 + -1.2 2,635,221 + -0.3 2,635,222 - -0.5 2,635,236 + +0.2 2,635,236 + -1.9 2,635,268 + -0.5 2,635,304 + +2.0 2,635,326 - -2.1 2,635,332 - -1.4 2,635,371 + BT2101 0.12 +0.0 2,635,372 - BT2101 0.12 +0.6 2,635,373 + BT2101 0.12 -0.6 2,635,390 - BT2101 0.13 -0.2 2,635,482 + BT2101 0.20 -2.4 2,635,491 + BT2101 0.21 -1.5 2,635,492 - BT2101 0.21 -2.1 2,635,524 - BT2101 0.23 +2.0 2,635,574 + BT2101 0.27 -1.0 2,635,611 - BT2101 0.30 -0.8 2,635,616 - BT2101 0.30 +1.0 2,635,617 + BT2101 0.30 -1.3 2,635,618 - BT2101 0.30 -2.8 2,635,618 - BT2101 0.30 +1.6 2,635,703 - BT2101 0.36 -0.5 2,635,740 + BT2101 0.39 -1.4 2,635,743 + BT2101 0.39 -0.9 2,635,821 - BT2101 0.45 +1.5 2,635,823 + BT2101 0.45 +2.0 2,635,824 - BT2101 0.45 +0.5 2,635,841 + BT2101 0.47 +0.0 2,635,846 + BT2101 0.47 -2.7 2,635,914 - BT2101 0.52 -0.4 2,635,934 - BT2101 0.53 -1.9 2,635,936 - BT2101 0.54 -1.3 2,635,940 + BT2101 0.54 -0.5 2,635,987 - BT2101 0.57 -1.7 2,635,987 - BT2101 0.57 +1.0 2,636,062 + BT2101 0.63 -1.5
Or see this region's nucleotide sequence