Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1944 and BT1945 overlap by 79 nucleotides BT1945 and BT1946 are separated by 92 nucleotides BT1946 and BT1947 are separated by 29 nucleotides BT1947 and BT1948 are separated by 76 nucleotides
BT1944: BT1944 - putative transposase (NCBI ptt file), at 2,445,566 to 2,445,970
BT1944
BT1945: BT1945 - conserved protein found in conjugate transposon (NCBI ptt file), at 2,445,892 to 2,446,674
BT1945
BT1946: BT1946 - hypothetical protein (NCBI ptt file), at 2,446,767 to 2,447,153
BT1946
BT1947: BT1947 - hypothetical protein (NCBI ptt file), at 2,447,183 to 2,447,599
BT1947
BT1948: BT1948 - hypothetical protein (NCBI ptt file), at 2,447,676 to 2,447,930
BT1948
Position (kb)
2446
2447
2448 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2445.785 kb on + strand, within BT1944 at 2445.793 kb on + strand, within BT1944 at 2445.842 kb on - strand, within BT1944 at 2445.894 kb on - strand at 2446.012 kb on - strand, within BT1945 at 2446.113 kb on - strand at 2446.134 kb on + strand, within BT1945 at 2446.215 kb on + strand, within BT1945 at 2446.243 kb on - strand, within BT1945 at 2446.269 kb on + strand, within BT1945 at 2446.270 kb on - strand, within BT1945 at 2446.271 kb on + strand, within BT1945 at 2446.368 kb on + strand, within BT1945 at 2446.371 kb on - strand, within BT1945 at 2446.371 kb on - strand, within BT1945 at 2446.602 kb on + strand at 2446.602 kb on + strand at 2446.696 kb on - strand at 2446.819 kb on + strand, within BT1946 at 2446.819 kb on + strand, within BT1946 at 2447.028 kb on - strand, within BT1946 at 2447.156 kb on + strand at 2447.431 kb on - strand, within BT1947 at 2447.483 kb on + strand, within BT1947 at 2447.694 kb on + strand at 2447.726 kb on - strand, within BT1948 at 2447.776 kb on - strand, within BT1948 at 2447.929 kb on - strand at 2447.957 kb on - strand at 2448.016 kb on - strand at 2448.016 kb on - strand at 2448.051 kb on + strand at 2448.059 kb on - strand at 2448.095 kb on - strand at 2448.100 kb on + strand at 2448.131 kb on - strand at 2448.131 kb on - strand at 2448.150 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 2,445,785 + BT1944 0.54 +1.0 2,445,793 + BT1944 0.56 +1.0 2,445,842 - BT1944 0.68 -1.3 2,445,894 - +1.0 2,446,012 - BT1945 0.15 -1.0 2,446,113 - -1.6 2,446,134 + BT1945 0.31 -1.1 2,446,215 + BT1945 0.41 +1.0 2,446,243 - BT1945 0.45 +3.0 2,446,269 + BT1945 0.48 -1.3 2,446,270 - BT1945 0.48 +0.0 2,446,271 + BT1945 0.48 -1.3 2,446,368 + BT1945 0.61 +0.3 2,446,371 - BT1945 0.61 -0.3 2,446,371 - BT1945 0.61 +1.7 2,446,602 + -0.7 2,446,602 + -1.3 2,446,696 - -1.8 2,446,819 + BT1946 0.13 -2.7 2,446,819 + BT1946 0.13 -2.3 2,447,028 - BT1946 0.67 -2.3 2,447,156 + +2.0 2,447,431 - BT1947 0.59 -1.4 2,447,483 + BT1947 0.72 +2.0 2,447,694 + -2.0 2,447,726 - BT1948 0.20 -0.2 2,447,776 - BT1948 0.39 -0.4 2,447,929 - +0.0 2,447,957 - -0.7 2,448,016 - -0.9 2,448,016 - -1.4 2,448,051 + -1.6 2,448,059 - +1.0 2,448,095 - +0.5 2,448,100 + -2.6 2,448,131 - -1.6 2,448,131 - +1.8 2,448,150 + -2.0
Or see this region's nucleotide sequence