Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1147 and BT1148 are separated by 202 nucleotides BT1148 and BT1149 are separated by 42 nucleotides BT1149 and BT1150 are separated by 21 nucleotides BT1150 and BT1151 are separated by 255 nucleotides
BT1147: BT1147 - glycine dehydrogenase [decarboxylating] (NCBI ptt file), at 1,429,971 to 1,432,820
BT1147
BT1148: BT1148 - putative NADH dehydrogenase/NAD(P)H nitroreductase (NCBI ptt file), at 1,433,023 to 1,433,592
BT1148
BT1149: BT1149 - Type II restriction enzyme HpaII (NCBI ptt file), at 1,433,635 to 1,434,720
BT1149
BT1150: BT1150 - conserved hypothetical protein (NCBI ptt file), at 1,434,742 to 1,435,350
BT1150
BT1151: BT1151 - conserved hypothetical protein (NCBI ptt file), at 1,435,606 to 1,436,853
BT1151
Position (kb)
1433
1434
1435 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1432.736 kb on + strand at 1432.736 kb on + strand at 1432.819 kb on - strand at 1432.846 kb on - strand at 1432.892 kb on + strand at 1432.915 kb on - strand at 1432.944 kb on + strand at 1433.014 kb on - strand at 1433.024 kb on + strand at 1433.054 kb on - strand at 1433.069 kb on - strand at 1433.096 kb on - strand, within BT1148 at 1433.111 kb on + strand, within BT1148 at 1433.111 kb on + strand, within BT1148 at 1433.113 kb on + strand, within BT1148 at 1433.113 kb on + strand, within BT1148 at 1433.119 kb on - strand, within BT1148 at 1433.325 kb on - strand, within BT1148 at 1433.422 kb on - strand, within BT1148 at 1433.538 kb on - strand at 1433.566 kb on - strand at 1433.595 kb on - strand at 1433.603 kb on + strand at 1433.612 kb on + strand at 1433.613 kb on - strand at 1433.678 kb on + strand at 1433.678 kb on + strand at 1433.679 kb on - strand at 1433.679 kb on - strand at 1433.679 kb on - strand at 1433.679 kb on - strand at 1433.708 kb on + strand at 1433.829 kb on + strand, within BT1149 at 1433.856 kb on + strand, within BT1149 at 1433.858 kb on + strand, within BT1149 at 1433.860 kb on + strand, within BT1149 at 1433.921 kb on + strand, within BT1149 at 1433.980 kb on + strand, within BT1149 at 1434.062 kb on - strand, within BT1149 at 1434.074 kb on - strand, within BT1149 at 1434.121 kb on + strand, within BT1149 at 1434.190 kb on - strand, within BT1149 at 1434.196 kb on - strand, within BT1149 at 1434.279 kb on + strand, within BT1149 at 1434.551 kb on - strand, within BT1149 at 1434.553 kb on - strand, within BT1149 at 1434.557 kb on + strand, within BT1149 at 1434.558 kb on - strand, within BT1149 at 1434.558 kb on - strand, within BT1149 at 1434.646 kb on + strand at 1434.722 kb on + strand at 1434.743 kb on + strand at 1434.819 kb on + strand, within BT1150 at 1434.883 kb on + strand, within BT1150 at 1434.888 kb on + strand, within BT1150 at 1434.955 kb on + strand, within BT1150 at 1434.973 kb on + strand, within BT1150 at 1435.080 kb on + strand, within BT1150 at 1435.202 kb on + strand, within BT1150 at 1435.202 kb on + strand, within BT1150 at 1435.205 kb on + strand, within BT1150 at 1435.302 kb on + strand at 1435.329 kb on + strand at 1435.400 kb on + strand at 1435.484 kb on - strand at 1435.592 kb on - strand at 1435.596 kb on - strand at 1435.599 kb on - strand at 1435.600 kb on + strand at 1435.600 kb on + strand at 1435.617 kb on + strand at 1435.674 kb on + strand at 1435.675 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 1,432,736 + +1.1 1,432,736 + +1.6 1,432,819 - +0.1 1,432,846 - -1.0 1,432,892 + -0.5 1,432,915 - -0.9 1,432,944 + -1.1 1,433,014 - -0.9 1,433,024 + -1.3 1,433,054 - +0.5 1,433,069 - -1.3 1,433,096 - BT1148 0.13 -1.0 1,433,111 + BT1148 0.15 -0.9 1,433,111 + BT1148 0.15 +1.1 1,433,113 + BT1148 0.16 +2.1 1,433,113 + BT1148 0.16 -0.5 1,433,119 - BT1148 0.17 -1.5 1,433,325 - BT1148 0.53 +1.1 1,433,422 - BT1148 0.70 +1.1 1,433,538 - -1.7 1,433,566 - +3.1 1,433,595 - +1.6 1,433,603 + -0.5 1,433,612 + -1.7 1,433,613 - +1.5 1,433,678 + +0.5 1,433,678 + +1.1 1,433,679 - -0.5 1,433,679 - -1.3 1,433,679 - -0.8 1,433,679 - -1.3 1,433,708 + +3.1 1,433,829 + BT1149 0.18 +1.6 1,433,856 + BT1149 0.20 -2.8 1,433,858 + BT1149 0.21 +0.6 1,433,860 + BT1149 0.21 -2.1 1,433,921 + BT1149 0.26 -1.1 1,433,980 + BT1149 0.32 -1.4 1,434,062 - BT1149 0.39 -1.3 1,434,074 - BT1149 0.40 -1.5 1,434,121 + BT1149 0.45 -3.0 1,434,190 - BT1149 0.51 -3.7 1,434,196 - BT1149 0.52 -3.5 1,434,279 + BT1149 0.59 -0.5 1,434,551 - BT1149 0.84 -1.2 1,434,553 - BT1149 0.85 -1.3 1,434,557 + BT1149 0.85 -2.8 1,434,558 - BT1149 0.85 -2.6 1,434,558 - BT1149 0.85 +1.1 1,434,646 + -0.9 1,434,722 + -1.3 1,434,743 + -1.4 1,434,819 + BT1150 0.13 -3.4 1,434,883 + BT1150 0.23 -1.9 1,434,888 + BT1150 0.24 -2.5 1,434,955 + BT1150 0.35 -0.0 1,434,973 + BT1150 0.38 +0.2 1,435,080 + BT1150 0.56 +2.6 1,435,202 + BT1150 0.76 -1.1 1,435,202 + BT1150 0.76 -0.4 1,435,205 + BT1150 0.76 +0.1 1,435,302 + -1.6 1,435,329 + -1.7 1,435,400 + +0.2 1,435,484 - +0.7 1,435,592 - -1.2 1,435,596 - -1.5 1,435,599 - -1.1 1,435,600 + -1.9 1,435,600 + -1.5 1,435,617 + -0.5 1,435,674 + -0.1 1,435,675 - +2.6
Or see this region's nucleotide sequence