Experiment: MoYLS4 with FCCP 2uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliN and DVU0047 are separated by 25 nucleotides DVU0047 and DVU0048 are separated by 20 nucleotides DVU0048 and DVU0049 overlap by 4 nucleotides
DVU0046: fliN - flagellar motor switch protein FliN (TIGR), at 52,090 to 52,602
fliN
DVU0047: DVU0047 - flagellar protein FliL (TIGR), at 52,628 to 53,143
DVU0047
DVU0048: DVU0048 - chemotaxis protein MotB (TIGR), at 53,164 to 53,904
DVU0048
DVU0049: DVU0049 - OmpA family protein (TIGR), at 53,901 to 54,995
DVU0049
Position (kb) 53
54 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 52.212 kb on - strand, within fliN at 52.226 kb on + strand, within fliN at 52.236 kb on + strand, within fliN at 52.257 kb on - strand, within fliN at 52.267 kb on - strand, within fliN at 52.335 kb on + strand, within fliN at 52.391 kb on + strand, within fliN at 52.392 kb on - strand, within fliN at 52.476 kb on + strand, within fliN at 52.527 kb on + strand, within fliN at 52.528 kb on + strand, within fliN at 52.576 kb on + strand at 52.584 kb on - strand at 52.602 kb on + strand at 52.619 kb on + strand at 52.687 kb on + strand, within DVU0047 at 52.705 kb on + strand, within DVU0047 at 52.718 kb on - strand, within DVU0047 at 52.769 kb on - strand, within DVU0047 at 52.792 kb on + strand, within DVU0047 at 52.841 kb on - strand, within DVU0047 at 52.939 kb on - strand, within DVU0047 at 53.014 kb on - strand, within DVU0047 at 53.130 kb on + strand at 53.151 kb on + strand at 53.154 kb on - strand at 53.192 kb on + strand at 53.281 kb on + strand, within DVU0048 at 53.282 kb on + strand, within DVU0048 at 53.290 kb on - strand, within DVU0048 at 53.307 kb on - strand, within DVU0048 at 53.374 kb on + strand, within DVU0048 at 53.410 kb on - strand, within DVU0048 at 53.410 kb on - strand, within DVU0048 at 53.430 kb on + strand, within DVU0048 at 53.487 kb on - strand, within DVU0048 at 53.570 kb on - strand, within DVU0048 at 53.570 kb on - strand, within DVU0048 at 53.574 kb on + strand, within DVU0048 at 53.580 kb on + strand, within DVU0048 at 53.583 kb on - strand, within DVU0048 at 53.595 kb on - strand, within DVU0048 at 53.596 kb on - strand, within DVU0048 at 53.603 kb on + strand, within DVU0048 at 53.603 kb on + strand, within DVU0048 at 53.644 kb on + strand, within DVU0048 at 53.662 kb on - strand, within DVU0048 at 53.700 kb on + strand, within DVU0048 at 53.708 kb on - strand, within DVU0048 at 53.708 kb on - strand, within DVU0048 at 53.767 kb on + strand, within DVU0048 at 53.777 kb on - strand, within DVU0048 at 53.850 kb on + strand at 53.858 kb on - strand at 53.953 kb on - strand at 53.953 kb on - strand at 53.953 kb on - strand at 53.962 kb on + strand at 53.965 kb on - strand at 54.105 kb on - strand, within DVU0049 at 54.274 kb on + strand, within DVU0049 at 54.282 kb on - strand, within DVU0049 at 54.290 kb on + strand, within DVU0049 at 54.456 kb on + strand, within DVU0049 at 54.464 kb on - strand, within DVU0049 at 54.482 kb on + strand, within DVU0049 at 54.503 kb on - strand, within DVU0049 at 54.514 kb on + strand, within DVU0049 at 54.543 kb on + strand, within DVU0049 at 54.553 kb on - strand, within DVU0049 at 54.563 kb on - strand, within DVU0049 at 54.566 kb on + strand, within DVU0049 at 54.596 kb on + strand, within DVU0049 at 54.605 kb on + strand, within DVU0049 at 54.614 kb on + strand, within DVU0049 at 54.624 kb on + strand, within DVU0049 at 54.646 kb on + strand, within DVU0049 at 54.677 kb on + strand, within DVU0049 at 54.685 kb on + strand, within DVU0049 at 54.685 kb on + strand, within DVU0049 at 54.693 kb on - strand, within DVU0049 at 54.693 kb on - strand, within DVU0049 at 54.707 kb on + strand, within DVU0049 at 54.712 kb on + strand, within DVU0049 at 54.730 kb on - strand, within DVU0049 at 54.778 kb on - strand, within DVU0049 at 54.809 kb on + strand, within DVU0049 at 54.809 kb on + strand, within DVU0049 at 54.817 kb on - strand, within DVU0049
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with FCCP 2uM remove 52,212 - fliN DVU0046 0.24 +1.3 52,226 + fliN DVU0046 0.27 +0.2 52,236 + fliN DVU0046 0.28 +0.5 52,257 - fliN DVU0046 0.33 -1.3 52,267 - fliN DVU0046 0.35 +0.5 52,335 + fliN DVU0046 0.48 +0.6 52,391 + fliN DVU0046 0.59 +1.9 52,392 - fliN DVU0046 0.59 -0.2 52,476 + fliN DVU0046 0.75 -0.7 52,527 + fliN DVU0046 0.85 +0.3 52,528 + fliN DVU0046 0.85 +0.5 52,576 + +0.3 52,584 - +0.5 52,602 + -0.9 52,619 + -0.1 52,687 + DVU0047 0.11 +0.8 52,705 + DVU0047 0.15 +0.1 52,718 - DVU0047 0.17 +0.8 52,769 - DVU0047 0.27 +0.5 52,792 + DVU0047 0.32 +0.6 52,841 - DVU0047 0.41 -2.1 52,939 - DVU0047 0.60 -1.1 53,014 - DVU0047 0.75 -0.3 53,130 + +0.1 53,151 + -1.3 53,154 - -0.1 53,192 + -0.4 53,281 + DVU0048 0.16 +2.9 53,282 + DVU0048 0.16 -0.3 53,290 - DVU0048 0.17 -0.0 53,307 - DVU0048 0.19 -1.1 53,374 + DVU0048 0.28 +1.1 53,410 - DVU0048 0.33 -0.1 53,410 - DVU0048 0.33 +2.2 53,430 + DVU0048 0.36 +0.3 53,487 - DVU0048 0.44 +0.9 53,570 - DVU0048 0.55 +1.7 53,570 - DVU0048 0.55 +0.6 53,574 + DVU0048 0.55 -0.1 53,580 + DVU0048 0.56 -0.7 53,583 - DVU0048 0.57 +0.1 53,595 - DVU0048 0.58 +0.6 53,596 - DVU0048 0.58 -0.7 53,603 + DVU0048 0.59 +0.6 53,603 + DVU0048 0.59 +0.1 53,644 + DVU0048 0.65 -0.7 53,662 - DVU0048 0.67 +0.9 53,700 + DVU0048 0.72 +0.3 53,708 - DVU0048 0.73 -0.1 53,708 - DVU0048 0.73 -0.1 53,767 + DVU0048 0.81 +0.3 53,777 - DVU0048 0.83 +0.6 53,850 + -0.6 53,858 - -0.1 53,953 - +0.2 53,953 - -0.1 53,953 - -0.1 53,962 + -1.1 53,965 - +0.1 54,105 - DVU0049 0.19 +0.9 54,274 + DVU0049 0.34 +0.4 54,282 - DVU0049 0.35 -0.5 54,290 + DVU0049 0.36 -0.5 54,456 + DVU0049 0.51 -0.7 54,464 - DVU0049 0.51 +0.2 54,482 + DVU0049 0.53 +0.5 54,503 - DVU0049 0.55 -0.7 54,514 + DVU0049 0.56 -0.3 54,543 + DVU0049 0.59 -0.0 54,553 - DVU0049 0.60 -0.8 54,563 - DVU0049 0.60 -1.7 54,566 + DVU0049 0.61 +0.1 54,596 + DVU0049 0.63 +0.1 54,605 + DVU0049 0.64 -0.1 54,614 + DVU0049 0.65 -0.2 54,624 + DVU0049 0.66 -1.1 54,646 + DVU0049 0.68 -0.2 54,677 + DVU0049 0.71 +0.0 54,685 + DVU0049 0.72 -0.7 54,685 + DVU0049 0.72 -0.2 54,693 - DVU0049 0.72 -0.5 54,693 - DVU0049 0.72 -0.1 54,707 + DVU0049 0.74 +0.0 54,712 + DVU0049 0.74 +0.3 54,730 - DVU0049 0.76 -0.7 54,778 - DVU0049 0.80 -0.3 54,809 + DVU0049 0.83 -0.7 54,809 + DVU0049 0.83 +1.2 54,817 - DVU0049 0.84 +0.9
Or see this region's nucleotide sequence