Experiment: Day4 LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1213 and SMa1214 are separated by 3 nucleotides SMa1214 and SMa1216 are separated by 12 nucleotides SMa1216 and SMa1220 are separated by 13 nucleotides SMa1220 and SMa1223 are separated by 314 nucleotides SMa1223 and SMa1225 are separated by 80 nucleotides
SMa1213: SMa1213 - FixP1 di-heme cytochrome c, at 667,360 to 668,229
SMa1213
SMa1214: SMa1214 - FixQ1 nitrogen fixation protein, at 668,233 to 668,385
SMa1214
SMa1216: SMa1216 - cbb3-type cytochrome c oxidase subunit II, at 668,398 to 669,129
SMa1216
SMa1220: SMa1220 - cbb3-type cytochrome c oxidase subunit I, at 669,143 to 670,762
SMa1220
SMa1223: SMa1223 - hypothetical protein, at 671,077 to 671,532
SMa1223
SMa1225: SMa1225 - transcriptional regulator FixK, at 671,613 to 672,248
SMa1225
Position (kb) 669
670
671 Strain fitness (log2 ratio) -2
-1
0
1 at 668.146 kb on - strand at 668.375 kb on + strand at 668.376 kb on - strand at 668.376 kb on - strand at 668.423 kb on + strand at 668.621 kb on + strand, within SMa1216 at 668.621 kb on + strand, within SMa1216 at 668.702 kb on + strand, within SMa1216 at 668.703 kb on - strand, within SMa1216 at 668.703 kb on - strand, within SMa1216 at 668.774 kb on + strand, within SMa1216 at 668.840 kb on + strand, within SMa1216 at 668.840 kb on + strand, within SMa1216 at 668.840 kb on + strand, within SMa1216 at 668.840 kb on + strand, within SMa1216 at 668.841 kb on - strand, within SMa1216 at 668.865 kb on - strand, within SMa1216 at 668.924 kb on + strand, within SMa1216 at 668.925 kb on - strand, within SMa1216 at 668.925 kb on - strand, within SMa1216 at 668.925 kb on - strand, within SMa1216 at 668.942 kb on + strand, within SMa1216 at 668.942 kb on + strand, within SMa1216 at 668.943 kb on - strand, within SMa1216 at 668.943 kb on - strand, within SMa1216 at 668.975 kb on + strand, within SMa1216 at 668.975 kb on + strand, within SMa1216 at 668.975 kb on + strand, within SMa1216 at 668.976 kb on - strand, within SMa1216 at 669.017 kb on + strand, within SMa1216 at 669.073 kb on + strand at 669.074 kb on - strand at 669.135 kb on - strand at 669.135 kb on - strand at 669.135 kb on - strand at 669.144 kb on + strand at 669.286 kb on - strand at 669.459 kb on + strand, within SMa1220 at 669.460 kb on - strand, within SMa1220 at 669.460 kb on - strand, within SMa1220 at 669.495 kb on + strand, within SMa1220 at 669.495 kb on + strand, within SMa1220 at 669.496 kb on - strand, within SMa1220 at 669.498 kb on + strand, within SMa1220 at 669.498 kb on + strand, within SMa1220 at 669.637 kb on - strand, within SMa1220 at 669.834 kb on + strand, within SMa1220 at 669.834 kb on + strand, within SMa1220 at 669.834 kb on + strand, within SMa1220 at 669.882 kb on + strand, within SMa1220 at 669.915 kb on + strand, within SMa1220 at 669.915 kb on + strand, within SMa1220 at 669.916 kb on - strand, within SMa1220 at 669.916 kb on - strand, within SMa1220 at 669.918 kb on - strand, within SMa1220 at 669.919 kb on - strand, within SMa1220 at 669.919 kb on - strand, within SMa1220 at 669.919 kb on - strand, within SMa1220 at 670.017 kb on + strand, within SMa1220 at 670.017 kb on + strand, within SMa1220 at 670.017 kb on + strand, within SMa1220 at 670.120 kb on - strand, within SMa1220 at 670.120 kb on - strand, within SMa1220 at 670.206 kb on + strand, within SMa1220 at 670.224 kb on + strand, within SMa1220 at 670.356 kb on + strand, within SMa1220 at 670.356 kb on + strand, within SMa1220 at 670.563 kb on + strand, within SMa1220 at 670.766 kb on - strand at 670.766 kb on - strand at 670.894 kb on + strand at 670.894 kb on + strand at 671.013 kb on + strand at 671.078 kb on + strand at 671.078 kb on + strand at 671.078 kb on + strand at 671.078 kb on + strand at 671.078 kb on + strand at 671.078 kb on + strand at 671.078 kb on + strand at 671.080 kb on + strand at 671.080 kb on + strand at 671.081 kb on - strand at 671.081 kb on - strand at 671.081 kb on - strand at 671.081 kb on - strand at 671.081 kb on - strand at 671.267 kb on - strand, within SMa1223 at 671.267 kb on - strand, within SMa1223 at 671.385 kb on + strand, within SMa1223 at 671.396 kb on + strand, within SMa1223
Per-strain Table
Position Strand Gene LocusTag Fraction Day4 LB remove 668,146 - -0.5 668,375 + -0.3 668,376 - -0.1 668,376 - -0.4 668,423 + -0.8 668,621 + SMa1216 0.30 -0.7 668,621 + SMa1216 0.30 -1.1 668,702 + SMa1216 0.42 -1.1 668,703 - SMa1216 0.42 +0.0 668,703 - SMa1216 0.42 -0.6 668,774 + SMa1216 0.51 -0.4 668,840 + SMa1216 0.60 -0.4 668,840 + SMa1216 0.60 -0.8 668,840 + SMa1216 0.60 +0.2 668,840 + SMa1216 0.60 -0.2 668,841 - SMa1216 0.61 -0.2 668,865 - SMa1216 0.64 -1.0 668,924 + SMa1216 0.72 -0.8 668,925 - SMa1216 0.72 -0.6 668,925 - SMa1216 0.72 -0.5 668,925 - SMa1216 0.72 +0.1 668,942 + SMa1216 0.74 -0.4 668,942 + SMa1216 0.74 -0.9 668,943 - SMa1216 0.74 -0.6 668,943 - SMa1216 0.74 -0.3 668,975 + SMa1216 0.79 -1.7 668,975 + SMa1216 0.79 -0.6 668,975 + SMa1216 0.79 -0.1 668,976 - SMa1216 0.79 -0.2 669,017 + SMa1216 0.85 -1.7 669,073 + -0.8 669,074 - -1.4 669,135 - -0.7 669,135 - -0.6 669,135 - -0.3 669,144 + -1.2 669,286 - -0.9 669,459 + SMa1220 0.20 -1.6 669,460 - SMa1220 0.20 -0.5 669,460 - SMa1220 0.20 -0.3 669,495 + SMa1220 0.22 -1.2 669,495 + SMa1220 0.22 -1.0 669,496 - SMa1220 0.22 -0.5 669,498 + SMa1220 0.22 -1.2 669,498 + SMa1220 0.22 -0.8 669,637 - SMa1220 0.30 -1.7 669,834 + SMa1220 0.43 -0.4 669,834 + SMa1220 0.43 -1.3 669,834 + SMa1220 0.43 -0.7 669,882 + SMa1220 0.46 -0.6 669,915 + SMa1220 0.48 -0.5 669,915 + SMa1220 0.48 -1.9 669,916 - SMa1220 0.48 -0.8 669,916 - SMa1220 0.48 -0.7 669,918 - SMa1220 0.48 -0.7 669,919 - SMa1220 0.48 -0.2 669,919 - SMa1220 0.48 -0.8 669,919 - SMa1220 0.48 -1.1 670,017 + SMa1220 0.54 +0.1 670,017 + SMa1220 0.54 +0.1 670,017 + SMa1220 0.54 -0.2 670,120 - SMa1220 0.60 -0.3 670,120 - SMa1220 0.60 +0.1 670,206 + SMa1220 0.66 -1.0 670,224 + SMa1220 0.67 -0.8 670,356 + SMa1220 0.75 -0.9 670,356 + SMa1220 0.75 -0.4 670,563 + SMa1220 0.88 -0.3 670,766 - -0.4 670,766 - -1.2 670,894 + -0.1 670,894 + +0.1 671,013 + +0.0 671,078 + +0.3 671,078 + +0.3 671,078 + +0.0 671,078 + +0.4 671,078 + +0.1 671,078 + +0.1 671,078 + +0.1 671,080 + +0.2 671,080 + +0.6 671,081 - +0.6 671,081 - -0.4 671,081 - -0.6 671,081 - +0.3 671,081 - -0.0 671,267 - SMa1223 0.42 -0.5 671,267 - SMa1223 0.42 +1.0 671,385 + SMa1223 0.68 +0.1 671,396 + SMa1223 0.70 +0.2
Or see this region's nucleotide sequence