Experiment: Day4 LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0007 and SMa0009 are separated by 38 nucleotides SMa0009 and SMa0011 are separated by 4 nucleotides SMa0011 and SMa0013 overlap by 92 nucleotides SMa0013 and SMa0015 overlap by 4 nucleotides
SMa0007: SMa0007 - formate dehydrogenase subunit gamma, at 5,424 to 6,110
SMa0007
SMa0009: SMa0009 - formate dehydrogenase subunit epsilon, at 6,149 to 7,069
SMa0009
SMa0011: SMa0011 - selenocysteine synthase, at 7,074 to 8,474
SMa0011
SMa0013: SMa0013 - hypothetical protein, at 8,383 to 9,255
SMa0013
SMa0015: SMa0015 - selenocysteine-specific elongation factor, at 9,252 to 11,252
SMa0015
Position (kb) 7
8
9 Strain fitness (log2 ratio) -1
0
1 at 6.166 kb on - strand at 6.296 kb on + strand, within SMa0009 at 6.297 kb on - strand, within SMa0009 at 6.620 kb on + strand, within SMa0009 at 6.620 kb on + strand, within SMa0009 at 6.620 kb on + strand, within SMa0009 at 6.620 kb on + strand, within SMa0009 at 6.621 kb on - strand, within SMa0009 at 6.621 kb on - strand, within SMa0009 at 6.621 kb on - strand, within SMa0009 at 6.861 kb on - strand, within SMa0009 at 6.944 kb on + strand, within SMa0009 at 7.028 kb on + strand at 7.028 kb on + strand at 7.196 kb on - strand at 7.340 kb on + strand, within SMa0011 at 7.341 kb on - strand, within SMa0011 at 7.695 kb on + strand, within SMa0011 at 7.696 kb on - strand, within SMa0011 at 7.696 kb on - strand, within SMa0011 at 7.742 kb on + strand, within SMa0011 at 7.743 kb on - strand, within SMa0011 at 7.752 kb on + strand, within SMa0011 at 7.872 kb on + strand, within SMa0011 at 7.873 kb on - strand, within SMa0011 at 8.086 kb on - strand, within SMa0011 at 8.249 kb on - strand, within SMa0011 at 8.490 kb on + strand, within SMa0013 at 8.491 kb on - strand at 8.491 kb on - strand, within SMa0013 at 8.491 kb on - strand, within SMa0013 at 8.558 kb on - strand, within SMa0013 at 8.731 kb on + strand, within SMa0013 at 9.007 kb on + strand, within SMa0013 at 9.007 kb on + strand, within SMa0013 at 9.008 kb on - strand, within SMa0013 at 9.008 kb on - strand, within SMa0013 at 9.008 kb on - strand, within SMa0013 at 9.008 kb on - strand, within SMa0013 at 9.075 kb on + strand, within SMa0013 at 9.382 kb on - strand at 9.449 kb on + strand at 9.449 kb on + strand at 9.449 kb on + strand at 9.450 kb on - strand at 9.450 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Day4 LB remove 6,166 - -0.2 6,296 + SMa0009 0.16 +0.3 6,297 - SMa0009 0.16 -0.1 6,620 + SMa0009 0.51 +0.1 6,620 + SMa0009 0.51 +0.6 6,620 + SMa0009 0.51 +0.3 6,620 + SMa0009 0.51 +0.1 6,621 - SMa0009 0.51 -0.1 6,621 - SMa0009 0.51 -0.8 6,621 - SMa0009 0.51 +0.5 6,861 - SMa0009 0.77 -0.0 6,944 + SMa0009 0.86 +0.4 7,028 + -0.2 7,028 + -1.7 7,196 - -0.3 7,340 + SMa0011 0.19 +0.3 7,341 - SMa0011 0.19 +1.5 7,695 + SMa0011 0.44 -0.0 7,696 - SMa0011 0.44 +0.0 7,696 - SMa0011 0.44 +1.4 7,742 + SMa0011 0.48 -0.2 7,743 - SMa0011 0.48 -0.4 7,752 + SMa0011 0.48 -0.1 7,872 + SMa0011 0.57 +0.5 7,873 - SMa0011 0.57 +0.0 8,086 - SMa0011 0.72 +1.0 8,249 - SMa0011 0.84 +0.0 8,490 + SMa0013 0.12 +0.1 8,491 - -0.4 8,491 - SMa0013 0.12 -0.2 8,491 - SMa0013 0.12 -0.1 8,558 - SMa0013 0.20 -0.2 8,731 + SMa0013 0.40 +0.0 9,007 + SMa0013 0.71 +0.1 9,007 + SMa0013 0.71 -0.2 9,008 - SMa0013 0.72 -0.0 9,008 - SMa0013 0.72 +0.5 9,008 - SMa0013 0.72 +0.0 9,008 - SMa0013 0.72 -0.5 9,075 + SMa0013 0.79 +0.2 9,382 - -0.1 9,449 + -0.5 9,449 + -0.3 9,449 + +0.7 9,450 - +0.3 9,450 - +0.1
Or see this region's nucleotide sequence