Experiment: Tween 20
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_702 and HP15_703 are separated by 469 nucleotides HP15_703 and HP15_tRNA14 are separated by 21 nucleotides HP15_tRNA14 and HP15_704 are separated by 92 nucleotides
HP15_702: HP15_702 - conserved hypothetical protein, at 710,723 to 711,118
_702
HP15_703: HP15_703 - phage integrase, at 711,588 to 712,454
_703
HP15_tRNA14: HP15_tRNA14 - tRNA-Met, at 712,476 to 712,552
_tRNA14
HP15_704: HP15_704 - translation-associated GTPase, at 712,645 to 713,736
_704
Position (kb) 711
712
713 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 710.753 kb on - strand at 710.755 kb on + strand at 710.939 kb on - strand, within HP15_702 at 710.949 kb on - strand, within HP15_702 at 710.956 kb on - strand, within HP15_702 at 710.956 kb on - strand, within HP15_702 at 710.980 kb on + strand, within HP15_702 at 710.995 kb on - strand, within HP15_702 at 711.019 kb on - strand, within HP15_702 at 711.038 kb on - strand, within HP15_702 at 711.059 kb on + strand, within HP15_702 at 711.067 kb on - strand, within HP15_702 at 711.144 kb on + strand at 711.162 kb on - strand at 711.265 kb on + strand at 711.461 kb on - strand at 711.537 kb on + strand at 711.582 kb on - strand at 711.613 kb on - strand at 711.854 kb on - strand, within HP15_703 at 711.856 kb on + strand, within HP15_703 at 711.861 kb on + strand, within HP15_703 at 711.861 kb on + strand, within HP15_703 at 711.874 kb on - strand, within HP15_703 at 711.904 kb on - strand, within HP15_703 at 712.064 kb on + strand, within HP15_703 at 712.177 kb on + strand, within HP15_703 at 712.180 kb on - strand, within HP15_703 at 712.180 kb on - strand, within HP15_703 at 712.240 kb on + strand, within HP15_703 at 712.300 kb on + strand, within HP15_703 at 712.334 kb on + strand, within HP15_703 at 712.342 kb on - strand, within HP15_703 at 712.617 kb on + strand at 713.172 kb on - strand, within HP15_704 at 713.366 kb on - strand, within HP15_704 at 713.384 kb on - strand, within HP15_704 at 713.384 kb on - strand, within HP15_704 at 713.412 kb on + strand, within HP15_704
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 remove 710,753 - -1.2 710,755 + +1.9 710,939 - HP15_702 0.55 -2.8 710,949 - HP15_702 0.57 +0.4 710,956 - HP15_702 0.59 -1.3 710,956 - HP15_702 0.59 -0.9 710,980 + HP15_702 0.65 -0.2 710,995 - HP15_702 0.69 +1.2 711,019 - HP15_702 0.75 +0.3 711,038 - HP15_702 0.80 +0.5 711,059 + HP15_702 0.85 -1.1 711,067 - HP15_702 0.87 +0.4 711,144 + -0.1 711,162 - +0.2 711,265 + -0.0 711,461 - -0.5 711,537 + -1.7 711,582 - -0.8 711,613 - +0.2 711,854 - HP15_703 0.31 +0.2 711,856 + HP15_703 0.31 -0.1 711,861 + HP15_703 0.31 -0.6 711,861 + HP15_703 0.31 -0.6 711,874 - HP15_703 0.33 -0.4 711,904 - HP15_703 0.36 -1.0 712,064 + HP15_703 0.55 +0.4 712,177 + HP15_703 0.68 +0.1 712,180 - HP15_703 0.68 +0.4 712,180 - HP15_703 0.68 +0.0 712,240 + HP15_703 0.75 -0.2 712,300 + HP15_703 0.82 -0.1 712,334 + HP15_703 0.86 -1.5 712,342 - HP15_703 0.87 -0.4 712,617 + +0.4 713,172 - HP15_704 0.48 -1.0 713,366 - HP15_704 0.66 -0.7 713,384 - HP15_704 0.68 -1.3 713,384 - HP15_704 0.68 +2.0 713,412 + HP15_704 0.70 -0.8
Or see this region's nucleotide sequence