Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05985

Experiment: L-Threonine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05975 and BWI76_RS05980 overlap by 8 nucleotidesBWI76_RS05980 and BWI76_RS05985 overlap by 14 nucleotidesBWI76_RS05985 and BWI76_RS05990 overlap by 1 nucleotidesBWI76_RS05990 and BWI76_RS05995 are separated by 20 nucleotides BWI76_RS05975: BWI76_RS05975 - ABC transporter ATP-binding protein, at 1,254,035 to 1,254,730 _RS05975 BWI76_RS05980: BWI76_RS05980 - ABC transporter ATP-binding protein, at 1,254,723 to 1,255,505 _RS05980 BWI76_RS05985: BWI76_RS05985 - branched-chain amino acid ABC transporter permease, at 1,255,492 to 1,256,541 _RS05985 BWI76_RS05990: BWI76_RS05990 - branched-chain amino acid ABC transporter permease, at 1,256,541 to 1,257,440 _RS05990 BWI76_RS05995: BWI76_RS05995 - branched-chain amino acid ABC transporter substrate-binding protein, at 1,257,461 to 1,258,597 _RS05995 Position (kb) 1255 1256 1257Strain fitness (log2 ratio) -3 -2 -1 0 1at 1254.675 kb on + strandat 1254.794 kb on - strandat 1254.816 kb on - strand, within BWI76_RS05980at 1254.856 kb on + strand, within BWI76_RS05980at 1255.055 kb on - strand, within BWI76_RS05980at 1255.099 kb on + strand, within BWI76_RS05980at 1255.099 kb on + strand, within BWI76_RS05980at 1255.182 kb on + strand, within BWI76_RS05980at 1255.338 kb on - strand, within BWI76_RS05980at 1255.352 kb on + strandat 1255.384 kb on + strand, within BWI76_RS05980at 1255.465 kb on + strandat 1255.831 kb on + strand, within BWI76_RS05985at 1256.049 kb on - strand, within BWI76_RS05985at 1256.193 kb on + strand, within BWI76_RS05985at 1256.366 kb on - strand, within BWI76_RS05985at 1256.382 kb on - strand, within BWI76_RS05985at 1256.441 kb on - strandat 1256.501 kb on - strandat 1256.713 kb on - strand, within BWI76_RS05990at 1256.756 kb on + strand, within BWI76_RS05990at 1256.775 kb on + strand, within BWI76_RS05990at 1256.786 kb on + strand, within BWI76_RS05990at 1256.809 kb on + strand, within BWI76_RS05990at 1256.809 kb on + strand, within BWI76_RS05990at 1256.889 kb on + strand, within BWI76_RS05990at 1257.244 kb on - strand, within BWI76_RS05990at 1257.336 kb on + strand, within BWI76_RS05990at 1257.396 kb on - strandat 1257.524 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Threonine (N)
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1,254,675 + -1.4
1,254,794 - -2.3
1,254,816 - BWI76_RS05980 0.12 -3.1
1,254,856 + BWI76_RS05980 0.17 +0.7
1,255,055 - BWI76_RS05980 0.42 -0.5
1,255,099 + BWI76_RS05980 0.48 -2.0
1,255,099 + BWI76_RS05980 0.48 -2.7
1,255,182 + BWI76_RS05980 0.59 -2.6
1,255,338 - BWI76_RS05980 0.79 -2.5
1,255,352 + +0.7
1,255,384 + BWI76_RS05980 0.84 -0.9
1,255,465 + -2.9
1,255,831 + BWI76_RS05985 0.32 -3.1
1,256,049 - BWI76_RS05985 0.53 -3.6
1,256,193 + BWI76_RS05985 0.67 -3.1
1,256,366 - BWI76_RS05985 0.83 -2.4
1,256,382 - BWI76_RS05985 0.85 -0.1
1,256,441 - -0.9
1,256,501 - -0.6
1,256,713 - BWI76_RS05990 0.19 -3.4
1,256,756 + BWI76_RS05990 0.24 -1.1
1,256,775 + BWI76_RS05990 0.26 -2.2
1,256,786 + BWI76_RS05990 0.27 -0.9
1,256,809 + BWI76_RS05990 0.30 -1.5
1,256,809 + BWI76_RS05990 0.30 -3.2
1,256,889 + BWI76_RS05990 0.39 -1.8
1,257,244 - BWI76_RS05990 0.78 -3.7
1,257,336 + BWI76_RS05990 0.88 -1.3
1,257,396 - -2.0
1,257,524 + -0.9

Or see this region's nucleotide sequence